Staphylococcus aureus (S. aureus) is a Gram-positive pathogen causing variety of infections in humans and animals. Extensive use antibiotics has led to the emergence methicillin-resistant S. (MRSA). As an alternative antibacterial agent against drug-resistant aureus, lytic phage, designated SLPW, was isolated from fecal sewage pig farm. The SLPW morphologically classified under Podoviridae contains double-stranded DNA genome. genome 17861 bp (29.35% G+C) containing 20 open reading frames...

Mastitis caused by Escherichia coli ( E. ) remains a threat to dairy animals and impacts animal welfare causes great economic loss. Furthermore, antibiotic resistance the lagged development of novel antibacterial drugs greatly challenge livestock industry. Phage therapy has regained attention. In this study, three lytic phages, termed vB_EcoM_SYGD1 (SYGD1), vB_EcoP_SYGE1 (SYGE1), vB_EcoM_SYGMH1 (SYGMH1), were isolated from sewage farm. The phages showed broad host range high bacteriolytic...

Bovine mastitis is a prevalent infectious disease in dairy herds worldwide, resulting substantial economic losses. Staphylococcus aureus major cause of animals, and its antibiotic resistance poses challenges for treatment. Recently, renewed interest has focused on the development alternative methods to therapy, including bacteriophages (phages), controlling bacterial infections. In this study, 2 lytic phages, vB_SauM_JDYN (JDYN) vB_SauM_JDF86 (JDF86), were isolated from cattle sewage...

Streptococcus suis is a significant zoonotic pathogen that great threat not only to the swine industry but also human health, causing arthritis, meningitis, and even streptococcal toxic shock-like syndrome. Owing its many serotypes high geographic variability, an efficacious cross-protective S. vaccine readily available. Therefore, this study aimed design universal multi-epitope (MVHP6) involved three highly immunogenic proteins of suis, namely, surface antigen containing glycosaminoglycan...

This research aimed to investigate the clinical features exhibited by individuals diagnosed with acute myocardial infarction (AMI) complicated ventricular septal rupture (VSR) and compare prognostic outcomes of different treatment modalities. A retrospective study on a cohort 200 patients who were AMI VSR at specialized medical facility from 2018 2023 was conducted. The categorized into 3 groups: group received management, B underwent surgical repair, C percutaneous device closure. Our...

ABSTRACT The use of virulent bacteriophages (phages) against pathogenic bacteria has recently attracted considerable interest. limitations naturally isolated phages have promoted the development genome engineering methods to optimize their functions; however, phage genomes in bacterial hosts remains challenging. Here, we describe a SMART ( s plitting, m odifying, ssembling, and r eboo t ing) method for multiplex by plitting into multiple segments cloned inserted single-copy artificial...

Deoxynivalenol (DON) is a secondary metabolite of fungi that harmful to humans and animals. This study examined the protective effects natural substances, including resveratrol, quercetin, vitamin E, C, microbe-derived antioxidants (MA), on both human gastric mucosal cells (GES-1) pig small intestinal epithelial (IPEC-1) when induced by DON. Cells were incubated with active substances for 3 h then exposed DON 24 h. The oxidative stress index, cell cycle, apoptosis measured. As compared...

ABSTRACT RIG-I and MDA5, known as the RIG-I-like receptors (RLRs), play a pivotal role in inducing antiviral responses to RNA viral infections. While chickens lack RIG-I, they possess functionally enhanced MDA5 that recognizes pathogens regulates immunity, underscoring critical of maintaining immune homeostasis chickens. However, precise mechanisms governing expression optimal activation remain unclear. Here, we reveal chicken E3 ubiquitin ligase RNF20 is essential for modulating...

Abstract Bats are natural hosts for various highly pathogenic viruses, which pose a considerable threat to humans and animals. However, they rarely display signs of disease infection from these viruses. The expression IRF7-induced IFN-β plays crucial role in preventing viral infections. the bat IRF7 during remains unclear. In this study, we cloned Tadarida brasiliensis discovered that its amino acid sequence was poorly conserved among species. Next, investigated mRNA lung cells (TB 1 Lu)...

Ginger (Zingiber officinale Roscoe), valued both for its medicinal and culinary uses, can be adversely affected by abiotic stresses such as high temperature drought, which impact growth development. The HSP90 gene family has been recognized a crucial element enhancing heat drought resistance in plants. Nevertheless, no studies have yet reported on the ginger. This study investigates ginger role plant’s responses to stresses. A total of 11 ZoHSP90 members were identified genome, these genes...

Chickens are the natural host of Newcastle disease virus (NDV) and avian influenza (AIV). The discovery that RIG-I gene, primary RNA pattern recognition receptor (PRR) in mammals, is naturally absent chickens has directed attention to studies chicken PRRs their functions antiviral immune responses. Here, we identified Asp-Glu-Ala-Asp (DEAD)-box helicase 1 (DDX1) as an essential PRR investigated its anti-RNA viral infections. chDDX1 gene was cloned, cross-species sequence alignment...

Bats are important hosts for various zoonotic viral diseases. However, they rarely show signs of disease infection with such viruses. As the first line virus control, innate immune system bats attracted our full attention. In this study, Tadarida brasiliensis MDA5 gene (batMDA5), a major sensor anti-RNA infection, was cloned, and its biological functions in antiviral immunity were identified. Bioinformatics analysis shows that amino acid sequence batMDA5 is poorly conserved among species, it...

Streptococcus suis (S. suis) is a gram-positive bacterium and zoonotic pathogen. Currently it poses serious problem in the swine industry due to emergence of antibiotic-resistant bacteria. Thus, novel antimicrobials against S. infections are urgently needed. In previous study, cell wall hydrolase or lysin derived from prophage phi5218, termed Ply5218, was identified. This showed strong bacteriolytic activity suis. current vitro data that after incubation with pig serum, efficacy Ply5218...

Pseudomonas aeruginosa is a Gram-negative pathogen that causes variety of infections in humans and animals. Due to the inappropriate use antibiotics, multi-drug resistant (MDR) P. strains have emerged are prevailing. In recent years, cow mastitis caused by MDR has attracted attention. this study, microbial community analysis revealed could be cause pathogen-induced mastitis. Five were isolated from milk diagnosed as positive. To seek an alternative antibacterial agent against MDR, , lytic...

Non-structural protein 1 (NS1) of influenza virus is a multifunctional that plays an important role in replication and virulence. In this study, acetylation modification was identified at the K108 residue NS1 H1N1 virus. To further explore function protein, deacetylation-mimic mutation (K108R) constant acetylation-mimic (K108Q) were introduced into background A/WSN/1933 (WSN), resulting two mutant viruses (WSN-NS1-108R WSN-NS1-108Q). vitro mouse studies showed K108R attenuated virulence...

Abstract Innate immunity plays an essential role in preventing the invasion of pathogenic microorganisms. However, innate is a double-edged sword, whose excessive activation detrimental to immune homeostasis and even leads “cytokine storm” infected host. The host develops series negative regulatory mechanisms balance response. Here, we report mechanism chicken mediated by miRNA. In GEO database, found that miR-126-5p was markedly up-regulated chickens RNA viruses. Upregulation virus then...

The ability of stimulator interferon genes (STING) to activate (IFN) responses during RNA virus infection has been demonstrated in different mammalian cells. Despite being the host numerous viruses, role STING bats not elucidated. In this study, we identified and cloned gene Brazilian free-tailed bat Tadarida brasiliensis ( T. ) tested its induce IFN-β by overexpressing knocking down (BatSTING) 1 lung (TB1 Lu) addition, used green fluorescent protein (GFP)-labeled vesicular stomatitis (VSV)...

Shiga toxin-converting bacteriophages (Stx phages) carry the stx gene and convert nonpathogenic bacterial strains into toxin-producing bacteria. There is limited understanding of effect that an Escherichia coli (E. coli) clustered regularly interspaced short palindromic repeats (CRISPR)-Cas adaptive immune system has on Stx phage lysogen. We investigated heat-stable nucleoid-structuring (H-NS) mutation-mediated CRISPR-Cas activation its E. Stx2 The Δhns mutant (MG1655Δhns) K-12 strain MG1655...