Jian Wang

ORCID: 0000-0001-9761-9747
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About
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Research Areas
  • Virus-based gene therapy research
  • Viral gastroenteritis research and epidemiology
  • Influenza Virus Research Studies
  • Microbial infections and disease research
  • Respiratory viral infections research
  • Streptococcal Infections and Treatments
  • Biosensors and Analytical Detection
  • Virology and Viral Diseases
  • Bacterial Identification and Susceptibility Testing
  • Bacteriophages and microbial interactions
  • Animal Virus Infections Studies
  • Molecular Biology Techniques and Applications
  • Rabies epidemiology and control
  • Carcinogens and Genotoxicity Assessment
  • Mycotoxins in Agriculture and Food
  • Yersinia bacterium, plague, ectoparasites research
  • Viral Infections and Vectors
  • Cervical Cancer and HPV Research
  • Ovarian cancer diagnosis and treatment
  • Plant Disease Resistance and Genetics
  • Human-Animal Interaction Studies
  • Animal Disease Management and Epidemiology

China Animal Disease Control Center
2012-2024

Urban stray cats are without owners that survive in the wild for extended periods of time. They one most common animals cities, and as such, monitoring pathogens carried by urban is an important component epidemiological surveillance. In order to understand prevalence respiratory diseases Shanghai provide scientific evidence development targeted prevention control strategies cats, we collected 374 ocular, nasal, oropharyngeal swabs from January 2022 December 2022. After RNA extraction, used...

10.3390/ani14111562 article EN cc-by Animals 2024-05-24

Streptococcus suis (S. suis) is a gram-positive bacterium and zoonotic pathogen. Currently it poses serious problem in the swine industry due to emergence of antibiotic-resistant bacteria. Thus, novel antimicrobials against S. infections are urgently needed. In previous study, cell wall hydrolase or lysin derived from prophage phi5218, termed Ply5218, was identified. This showed strong bacteriolytic activity suis. current vitro data that after incubation with pig serum, efficacy Ply5218...

10.3390/v11080715 article EN cc-by Viruses 2019-08-05

MALDI fingerprinting was first described two decades ago as a technique to identify microbial cell lines. Microbial has since evolved into an automated platform for microorganism identification and classification, which is now routinely used in clinical environmental sectors. The extension of mammalian cells yet progress partly due compartmentalization eukaryotic overall higher cellular complexity. A number publications on whole suggest that the method could be useful classification...

10.1007/s13361-018-2088-6 article EN Journal of the American Society for Mass Spectrometry 2018-10-23

In this study, one G2c-subtype strain of porcine epidemic diarrhea virus (PEDV) (SHXX1902 strain) was isolated from clinical samples in suspended Vero cells, which different the genotype commercial AJ1102 vaccine. As a result, we determined pathogenicity passages' isolates and compared immunogenicity with The viral titer reached 107 50% tissue culture infectious dose (TCID50)/ml, met requirement for seed replication during vaccine development. Five-day-old piglets were orally infected...

10.3389/fmicb.2022.864377 article EN cc-by Frontiers in Microbiology 2022-04-12

Mass spectrometry (MS) offers high levels of specificity and sensitivity in clinical applications, we have previously been able to demonstrate that matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS is capable distinguishing two-component cell mixtures at low limits detection. Ovarian cancer notoriously difficult detect due the lack diagnostic techniques available medical community. By sampling a local microenvironment, such as vaginal canal cervix, based method...

10.1021/acs.jproteome.9b00694 article EN Journal of Proteome Research 2019-11-18

Rural dog populations have long been recognized to be inadequately managed in terms of disease control and prevention. In this study we consider management rural Shanghai its implications for rabies the entire metropolitan area Shanghai. The prerequisite improve vaccination coverage depends on a proper enumeration total population. selected one nine administrative districts (Jiading), within which there are 7 towns 2 industrial zones (township-level division) that contain agricultural areas....

10.3389/fvets.2021.630180 article EN cc-by Frontiers in Veterinary Science 2021-07-05

To construct a protein fingerprint database of Haemophilus parasuis (H. parasuis), thus improving its clinical diagnosis efficiency. A total 15 H. standard strains were collected to establish using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), and the effects different culture media time on quality identification results investigated. The showed that tryptone soy agar (TSA) broth (TSB) incubation times had no significant effect characteristic...

10.1155/2021/5588855 article EN cc-by BioMed Research International 2021-04-15

Abstract A rapid and sensitive assay is essential to promote reliable surveillance diagnosis of canine astrovirus (CaAstV). In this study, two real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays with high sensitivity, rapidity reliability were developed using fluorescence dye FRET-Based Assimilating Probes for CaAstV detection. Two real time RT-LAMP specifically amplified the ORF2 gene did not amplify other enterovirus nucleic acids. The limit detection...

10.21203/rs.3.rs-3026743/v1 preprint EN cc-by Research Square (Research Square) 2023-06-26
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