A5100769112- Herpesvirus Infections and Treatments
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Hepatitis B Virus Studies
- Influenza Virus Research Studies
- Animal Disease Management and Epidemiology
- Cytomegalovirus and herpesvirus research
- Respiratory viral infections research
- Viral Infections and Immunology Research
- Virology and Viral Diseases
- Proteoglycans and glycosaminoglycans research
- Mosquito-borne diseases and control
- Hepatitis C virus research
- Viral Infections and Vectors
- Liver Disease Diagnosis and Treatment
- Hepatitis Viruses Studies and Epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- Mycobacterium research and diagnosis
- Cancer-related molecular mechanisms research
- Leprosy Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
- interferon and immune responses
- Circular RNAs in diseases
Qingdao University
2025
Affiliated Hospital of Qingdao University
2025
China Animal Disease Control Center
2009-2024
Fifth Affiliated Hospital of Sun Yat-sen University
2017-2024
Second Hospital of Hebei Medical University
2023-2024
Hebei Medical University
2023-2024
Zhangzhou Normal University
2019-2024
Sun Yat-sen University
2017-2024
Ningxia Medical University
2010-2024
Sichuan University
2014-2023
Herpes simplex virus type 1 (HSV-1) infection of the corneal stroma remains a major cause blindness. Primary cultures fibroblasts (CF) were tested and found susceptible to HSV-1 entry, which was confirmed by deconvolution imaging infected cells. Plaque assay real-time PCR demonstrated viral replication hence productive CF HSV-1. A role for glycoprotein D (gD) receptors in cultured determined gD interference assay. Reverse transcription-PCR analysis indicated expression herpesvirus entry...
Heparan sulfate (HS) is a highly sulfated polysaccharide and present in large quantities on the cell surface extracellular matrix. Herpes simplex virus type 1 (HSV-1) utilizes specialized HS, known as 3-O-sulfated an entry receptor to establish infection. Here, we exploit approach inhibiting HSV-1 infection by using octasaccharide, mimicking active domain of receptor. The octasaccharide was synthesized incubating heparin (3-OH octasaccharide) with HS 3-O-sulfotransferase isoform 3. resultant...
Heparan sulfate (HS) and its highly modified form, 3-O-sulfated heparan (3-OS HS), contribute strongly to herpes simplex virus type-1 (HSV-1) infection in vitro. Here we report results from a random M13-phage display library screening isolate 12-mer peptides that bind specifically HS, 3-OS block HSV-1 entry. The identified representative candidates two-different groups of anti-HS with high positive charge densities. Group 1, represented by G1 peptide (LRSRTKIIRIRH), belongs class alternating...
Zika virus (ZIKV) has been defined as a teratogenic pathogen behind the increased number of cases microcephaly in French Polynesia, Brazil, Puerto Rico, and other South American countries. Experimental studies using animal models have achieved tremendous insight into understanding viral pathogenesis, transmission, mechanisms, virus–host interactions. However, animals used published investigations are mostly interferon (IFN)-compromised, either genetically or via antibody treatment. Herein,...
Phenotypic whole-cell screening in erythrocytic cocultures of Plasmodium falciparum identified a series dihydroisoquinolones that possessed potent antimalarial activity against multiple resistant strains P. vitro and show no cytotoxicity to mammalian cells. Systematic structure-activity studies revealed relationships between potency modifications at N-2, C-3, C-4. Careful structure-property relationship studies, coupled with metabolism, addressed the poor aqueous solubility metabolic...
As one of the most favorable stimuli, photoactivation provides an advantageous way to manipulate biological objects. In current study, we have successfully demonstrated use light activation guide RNA (gRNA) strategy for controlling CRISPR systems. By conjugating photolabile protecting groups, functions became minimal, but exposure acylated gRNAs 365 nm triggers removal masking leading rescue functions. Furthermore, our has been used control gene editing in human cells. This proof-of-concept...
Human enteroviruses (EVs) are the most common causative agents infecting human, causing many harmful diseases, such as hand, foot, and mouth disease (HFMD), herpangina (HA), myocarditis, encephalitis, aseptic meningitis. EV-related diseases pose a serious worldwide threat to public health. To gain comprehensive insight into seroepidemiology of major prevalent EVs in humans, we firstly performed serological survey for neutralizing antibodies (nAbs) against Enterovirus A71 (EV-A71), Coxsackie...
Abstract Varicella caused by the primary infection of varicella-zoster virus (VZV) exerts a considerable disease burden globally. Current varicella vaccines consisting live-attenuated vOka strain VZV are generally safe and effective. However, retains full neurovirulence can establish latency reactivate to cause herpes zoster in vaccine recipients, raising safety concerns. Here, we rationally design candidate, v7D. This replicates like wild-type MRC-5 fibroblasts human PBMCs, carrier for...
H9N2 influenza viruses have become established and maintain long-term endemicity in poultry. The complete genomes of seven avian were characterized. These virus isolates obtained from live poultry markets Shanghai, China, 2002 2006 to 2008. Genetic analysis revealed that all had an RSSR motif at the cleavage site hemagglutinin (HA), indicating low pathogenicity chickens. Phylogenetic analyses indicated belonged lineage represented by Duck/Hong Kong/Y280/97 (H9N2), a belonging...
Background As a genetic disorder of abnormal pigmentation, the molecular basis dyschromatosis universalis hereditaria (DUH) had remained unclear until recently when ABCB6 was reported as causative gene DUH. Methodology We performed genome-wide linkage scan using Illumina Human 660W-Quad BeadChip and exome sequencing analyses Agilent SureSelect All Exon Kits in multiplex Chinese DUH family to identify pathogenic mutations verified candidate Sanger sequencing. Quantitative RT-PCR...
Human cytomegalovirus (HCMV) infects a large portion of the human population globally. Several HCMV-derived noncoding RNAs are involved in regulation viral gene expression and virus life cycle. Here, we reported that circRNAs new class HCMV transcripts. We bioinformatically predict 704 candidate encoded by TB40/E strain 230 HAN strain. also systematically compare circRNA features, including breakpoint sequence consensus, strand preference, length distribution, exon numbers between host...
Digitalization provides opportunities for sustainable development. Cultivating postgraduates’ digital skills is an important task of higher education to support development (HESD). As a crucial way cultivating skills, high-quality online learning processes are great significance achieve “Quality Education”, in line with the 2030 agenda. Based on Biggs’s 3P (Presage-Process-Product) model, this study focused whole process and explored relationship among information literacy, platforms,...
Herpes simplex virus type 1 (HSV-1) interaction with glycoprotein D (gD) receptors facilitates entry into cells. Chinese hamster ovary (CHO-K1) cells lacking cellular allow to attach, but not enter, implying a role for during the post-attachment (entry) phase of HSV-1 infection. Here, it is shown that presence soluble heparan sulfate (HS) modified by 3-O-sulfotransferase-3 (3-OST-3), 3-OST-1, triggered resistant CHO-K1 It was further demonstrated mutant deficient in glycosaminoglycan...