A5100421525- DNA Repair Mechanisms
- RNA regulation and disease
- RNA modifications and cancer
- RNA Research and Splicing
- Redox biology and oxidative stress
- Cancer-related Molecular Pathways
- Ubiquitin and proteasome pathways
- Phosphodiesterase function and regulation
- Histone Deacetylase Inhibitors Research
- Connexins and lens biology
- Advanced Glycation End Products research
- Acute Lymphoblastic Leukemia research
- Advanced biosensing and bioanalysis techniques
- PARP inhibition in cancer therapy
- Glioma Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Ferroptosis and cancer prognosis
- Cellular Mechanics and Interactions
- Chemical Synthesis and Analysis
- Sulfur Compounds in Biology
- interferon and immune responses
- Viral Infectious Diseases and Gene Expression in Insects
- Nuclear Structure and Function
- Natural product bioactivities and synthesis
- Carcinogens and Genotoxicity Assessment
Chongqing Medical University
2015-2025
Sichuan University
2023-2025
Southwest Jiaotong University
2024-2025
Chengdu Third People's Hospital
2024-2025
Hunan Normal University
2024
Shanxi Medical University
2024
State Key Laboratory of Oral Diseases
2023
Tianjin Medical University Cancer Institute and Hospital
2023
Lanzhou University
2023
Huazhong University of Science and Technology
2011-2022
cAMP is a key second messenger that regulates diverse cellular functions including neural plasticity. However, the spatiotemporal dynamics of intracellular in intact organisms are largely unknown due to low sensitivity and/or brightness current genetically encoded fluorescent indicators. Here, we report development new circularly permuted GFP (cpGFP)-based indicator G-Flamp1, which exhibits large fluorescence increase (a maximum ΔF/F
Abstract The etiology of esophageal squamous cell carcinoma (ESCC) has been shown to be associated with genetic and certain environmental factors that produce DNA damage. Base excision repair (BER) genes are responsible for damage caused by reactive oxygen species other electrophiles therefore good candidate susceptibility ESCC. We first screened eight BER new potential functional polymorphisms resequencing 27 samples. then identified genotyped important tagging single nucleotide (SNPs) in a...
This study introduces a novel classification approach that combines convolutional neural network (CNN) and Raman mapping to differentiate between tongue squamous cell carcinoma (TSCC) non-tumorous tissue, as well identify different histological grades of TSCC. In this study, 240 mappings data from 30 tissue samples were collected 15 patients who had undergone surgical resection for A total 18,000 sub-mappings extracted then used train test CNN model, which feature representations...
A-to-I RNA editing and m6A modification are two of the most prevalent types modifications controlling gene expression in mammals play very important roles tumorigenesis tumor progression. However, functional correlations these remain to be further investigated cancer. Herein, we show that ADAR1, an RNA-editing enzyme, interacts with METTL3 increases its protein level promote proliferation, migration invasion breast cancer cells through a mechanism connecting YTHDF1. We both ADAR1 upregulated...
Abstract Betulinic acid (BA) exhibits cytotoxic activity against some cancer cells. However, the molecular mechanism of BA CRC cells was little reported. Here, we proved that elicited cells' growth inhibition and apoptosis in a dose-dependent manner. In addition, treatment induced autophagy via inhibiting AKT-MTOR signaling pathway. Inhibition by either administration autophagic inhibitor chloroquine or siRNA-mediated knockdown ATG5 could augment BA-induced apoptotic cell death as well...
Parkinson's disease (PD) is a quite common neurodegenerative disorder with prevalence of approximately 1:800-1,000 in subjects over 60 years old. The aim our study was to determine the candidate target genes PD through meta-analysis multiple gene expression arrays datasets and further combine mRNA miRNA analyses identify more convincing biological targets their regulatory factors. Six included were obtained from Gene Expression Omnibus database by systematical search, including five (150...
DEPDC1 is a recently identified novel tumor-related gene that upregulated in several types of cancer and contributes to tumorigenesis. In this study, we have investigated the expression pattern functional implications during cell cycle progression. Expression studies using synchronized cells demonstrated highly expressed mitotic phase cycle. Immunofluorescence assays showed predominantly localized nucleus interphase redistributed into whole upon nuclear membrane breakdown metaphase....
Methionine sulfoxide reductase B1 (MSRB1) is involved in the development and immune regulation of multiple tumors. However, role MSRB1 tumor microenvironment its potential as a therapeutic target remain largely unknown. In this study, expression patterns were evaluated using pan-cancer RNA sequencing data from cell lines, tissues, single cells. The prognostic was assessed association between certain cancer characteristics analyzed. We showed that levels increased several types (
Background As a genetic disorder of abnormal pigmentation, the molecular basis dyschromatosis universalis hereditaria (DUH) had remained unclear until recently when ABCB6 was reported as causative gene DUH. Methodology We performed genome-wide linkage scan using Illumina Human 660W-Quad BeadChip and exome sequencing analyses Agilent SureSelect All Exon Kits in multiplex Chinese DUH family to identify pathogenic mutations verified candidate Sanger sequencing. Quantitative RT-PCR...
Autoimmune hepatitis (AIH) is an autoimmune disease accompanied by inflammatory response that often leads to severe liver damage. In addition, it may further lead complications such as fibrosis, cirrhosis and failure. Dihydromyricetin (DHM) possesses various pharmacological properties, being anti-inflammatory, antioxidant, antibacterial. this experiment, we investigated the effect of DHM on mice based level M1/M2 type macrophages. An mouse model was established administration followed tail...
A serious game module called "D-Casting" was developed in the previous study. This study aimed to determine effectiveness of D-Casting module. The experiment consisted two parts: construct validity assessment and skill transfer assessment. Eligible participants, who were students majoring dental technology, recruited from Stomatology College Chongqing Medical University. intervention designed based on games framework. a total 145 participants (100% response rate). results suggested that...
Insects can effortlessly walk on various substrate surfaces using attachment pads their feet, even upside down smooth surfaces. These undergo frequent deformation throughout the lifecycle of insects to achieve stable adhesion. Interest in insect adhesion spans 100s years; studies have indicated special mechanical properties and intricate structural design pads. However, genetic basis function remains unclear. Resilin, an elastic protein widely distributed exoskeletons, plays a crucial role...
Abstract Temozolomide (TMZ), a DNA alkylating agent, has become the primary treatment for glioma, most common malignancy of central nervous system. Although TMZ-containing regimens produce significant clinical response rates, some patients inevitably suffer from inferior outcomes or disease relapse, likely because poor chemosensitivity glioma cells due to robust damage (DDR). GINS2, subunit helicase, contributes maintaining genomic stability and is highly expressed in various cancers,...
Gallstones are crystalline deposits in the gallbladder that traditionally classified as cholesterol, pigment, or mixed stones based on their composition. Microbiota and host metabolism variances among different types of gallstones remain largely unclear. Here, bile gallstone microbial species spectra 29 subjects with disease (GSD, 24 cholesterol 5 pigment) were revealed by type IIB restriction site-associated DNA microbiome sequencing (2bRAD-M). Among them (21 subjects: 18 3 pigment), plasma...
p53 deficiency plays a crucial role in chemotherapy resistance through various biological events, including posttranslational modifications (PTMs). Recently, lysine crotonylation (Kcr) has been shown to play vital cancer progression. However, the global p53-regulated crotonylome and function of these altered Kcr proteins after remain unclear. In this study, we used SILAC-based quantitative identify 3,520 1924 crotonylated response knockout. We found that increased RRM2 at K283 (RRM2K283Cr)...
The presence of loss-of-heterozygosity (LOH) mutations in cancer cell genomes is commonly encountered. Moreover, the occurrences LOHs tumor suppressor genes play important roles oncogenesis. However, because causative mechanisms underlying LOH cells yet remain to be elucidated, enquiry into nature these based on a comprehensive examination characteristics multiple types cancers has become necessity. We performed next-generation sequencing inter-Alu sequences five different solid tumors and...
Abstract Bleomycin, a widely used anti-cancer drug, may give rise to pulmonary fibrosis, serious side effect which is associated with significant morbidity and mortality. Despite the intensive efforts, precise pathogenic mechanisms of fibrosis still remain be clarified. Our previous study showed that bleomycin bound directly annexin A2 (ANXA2, or p36), leading development by impeding transcription factor EB (TFEB)-induced autophagic flux. Here, we demonstrated ANXA2 also played critical role...
DNA integrity plays a crucial role in cell survival. Methamphetamine (METH) is an illegal psychoactive substance that abused worldwide, and repeated exposure to METH could form mass free radicals induce neuronal apoptosis. It has been reported generated by treatment can oxidize hence produce strand breaks, but whether oxidative damage involved the neurotoxicity caused remains unclear. Tea polyphenols exert bioactivities through antioxidant-related mechanisms. However, potential...
8-Chloro-adenosine (8-Cl-Ado) has been shown to exhibit its antitumor activity by inducing apoptosis in human lung cancer A549 and H1299 cells or autophagy chronic lymphocytic leukemia, MDA-MB-231 MCF-7 breast cells. Adenosine deaminases acting on RNA 1 (ADAR1) is tightly associated with development progression. The aim of this study was investigate the role ADAR1 proliferation SK-BR-3 cell lines after 8-Cl-Ado exposure possible mechanisms. After exposure, CCK-8 assay performed determine...
Unscheduled R-loops are a major source of replication stress and DNA damage. R-loop-induced defects sensed suppressed by ATR kinase, whereas it is not known whether R-loop itself actively involved in activation and, if so, how this achieved. Here, we report that the nuclear form RNA-editing enzyme ADAR1 promotes resolves genome-wide R-loops, process requires its double-stranded RNA-binding domains. Mechanistically, interacts with TOPBP1 facilitates loading on perturbed forks enhancing...
The actin cytoskeleton is extremely dynamic and supports diverse cellular functions in many physiological pathological processes, including tumorigenesis. However, the mechanisms that regulate actin-related protein 2/3 (ARP2/3) complex thereby promote polymerization organization cancer cells are not well-understood. We previously implicated proline-rich 11 (PRR11) lung development. In this study, using immunofluorescence staining, assays, siRNA-mediated gene silencing, we uncovered...