A5086673818- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- DNA Repair Mechanisms
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- DNA and Nucleic Acid Chemistry
- Molecular Biology Techniques and Applications
- Genetics and Neurodevelopmental Disorders
- Enzyme Structure and Function
- Free Radicals and Antioxidants
- Glycosylation and Glycoproteins Research
- Epigenetics and DNA Methylation
- Phytochemicals and Antioxidant Activities
- Antimicrobial Peptides and Activities
- Ubiquitin and proteasome pathways
- Plant Virus Research Studies
- Nuclear Structure and Function
- Phytochemical and Pharmacological Studies
Lomonosov Moscow State University
2021-2025
Institute of Gene Biology
2023-2025
Moscow State University
2022-2023
Fox Chase Cancer Center
2021
FACT is a histone chaperone that participates in nucleosome removal and reassembly during transcription replication. We used electron microscopy to study FACT, FACT:Nhp6 FACT:Nhp6:nucleosome complexes, found all complexes adopt broad ranges of configurations, indicating high flexibility. unexpectedly the DNA binding protein Nhp6 also binds C-terminal tails subunits, inducing more open geometries even absence nucleosomes. therefore supports unfolding by altering both structure properties...
Abstract During various DNA-centered processes in the cell nucleus, minimal structural units of chromatin organization, nucleosomes, are often transiently converted to hexasomes and tetrasomes missing one or both H2A/H2B histone dimers, respectively. However, functional properties subnucleosomes their impact on biological nuclei poorly understood. Here, using biochemical approaches, molecular dynamics simulations, single-particle Förster resonance energy transfer microscopy, nuclear magnetic...
In eukaryotic organisms, genomic DNA associates with histone proteins to form nucleosomes. Nucleosomes provide a basis for genome compaction, epigenetic markup, and mediate interactions of nuclear their target loci. A negatively charged (acidic) patch located on the H2A-H2B dimer is characteristic feature nucleosomal surface. The acidic common site in attachment various chromatin proteins, including viral ones. Acidic patch-binding peptides present perspective compounds that can be used...
Yeast Hmo1 is a high mobility group B (HMGB) protein that participates in the transcription of ribosomal genes and rDNA, also stimulates activities some ATP-dependent remodelers. binds both DNA nucleosomes has been proposed to be functional yeast analog mammalian linker histones. We used EMSA single particle Förster resonance energy transfer (spFRET) microscopy characterize effects on alone with histone chaperone FACT. induced significant increase distance between gyres across nucleosomal...
Human FACT (FACT) is a multifunctional histone chaperone involved in transcription, replication and DNA repair. Curaxins are anticancer compounds that induce FACT-dependent nucleosome unfolding trapping of the chromatin cancer cells (c-trapping) through an unknown molecular mechanism. Here, we analyzed effects curaxin CBL0137 on by using spFRET electron microscopy. By itself, adopted multiple conformations, including novel, compact, four-domain state which previously unresolved NTD SPT16...
Inorganic ions are essential factors stabilizing nucleosome structure; however, many aspects of their effects on DNA transactions in chromatin remain unknown. Here, differential K + and Na the structure, stability, interactions with protein complex FACT (FAcilitates Chromatin Transcription), poly(ADP-ribose) polymerase 1, RNA II were studied using primarily single-particle Förster resonance energy transfer microscopy. The maximal effect a structure was observed at ca. 80–150 mM, it decreased...
During various DNA-centered processes in the cell nucleus, minimal structural units of chromatin organization, nucleosomes, are often transiently converted to hexasomes and tetrasomes missing one or both H2A/H2B histone dimers, respectively. However, functional properties subnucleosomes their impact on biological nuclei poorly understood. Here, using biochemical approaches, molecular dynamics simulations, single-particle Förster resonance energy transfer (spFRET) microscopy NMR spectroscopy,...
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Genetic apparatus of human cells is constantly affected by a broad spectrum mutagenic factors, both exogenous and endogenous. epigenetic disorders, which emerge as result this influence, become the main cause majority malignant neoplasias. Several different approaches were proposed to prevent these including suppression activity factors treatment with certain chemical compounds. Plant polyphenols are promising candidates for development chemopreventive drugs, they exert ability regulate...
Abstract Human FACT (FACT) is a multifunctional histone chaperone involved in transcription, replication and DNA repair. Curaxins are anticancer compounds that induce FACT- dependent nucleosome unfolding trapping of the chromatin cancer cells (c-trapping) through an unknown molecular mechanism. Here, we analyzed effects curaxin CBL0137 on by using spFRET electron microscopy. By itself, adopted multiple conformations, including novel, compact, four-domain state which previously unresolved NTD...
Abstract Human FACT (FACT) is a multifunctional histone chaperone involved in transcription, replication and DNA repair. Curaxins are anticancer compounds that induce FACT-dependent nucleosome unfolding trapping of the chromatin cancer cells (c-trapping) through an unknown molecular mechanism. Here, we analyzed effects curaxin CBL0137 on by using spFRET electron microscopy. By itself, adopted multiple conformations, including novel, compact, four-domain state which previously unresolved NTD...
Abstract FACT is a histone chaperone that unfolds nucleosomes without ATP hydrolysis. We used electron microscopy to study and FACT:nucleosome complexes, found both adopt broad ranges of configurations, indicating high flexibility. unexpectedly the DNA binding protein Nhp6 also binds C-terminal tails subunits, inducing more open geometries even in absence nucleosomes. therefore supports nucleosome unfolding by altering structure properties. Complexes formed with FACT, Nhp6, produced range...
An abstract is not available for this content so a preview has been provided. As you have access to content, full PDF via the ‘Save PDF’ action button.
Background: DNA accessibility in chromatin is important for proper gene expression and regulated by multiple factors. One of them histone chaperone FACT, which conducts large-scale ATP-independent nucleosome unfolding that increases the nucleosomal DNA. FACT binding results dramatic uncoiling from nucleosome, occurs without apparent loss histones, proceeds via an ‘all-or-none’ mechanism, but detailed mechanism this process still unknown. FACT-dependent modulates DNA, it function processes...
Background: Histone chaperon FACT (“FAcilitates Chromatin Transcription”) is a multifunctional and conserved eukaryotic protein involved in DNA transcription, replication repair; which can reversibly unfold nucleosomes presence of ATP. necessary for the viability growth breast tumor cells meanwhile normal it be knocked out without loss vitality. Human (hFACT) target promising anticancer drug curaxins, causes trapping chromatin cancer destabilizes nucleosome. The nucleosome-unfolding activity...
Abstract FACT is a histone chaperone that unfolds nucleosomes without ATP hydrolysis. We used electron microscopy to study and FACT:nucleosome complexes, found both adopt broad ranges of configurations, indicating high flexibility. unexpectedly the DNA binding protein Nhp6 also binds C-terminal tails subunits, inducing more open geometries even in absence nucleosomes. therefore supports nucleosome unfolding by altering structure properties. Complexes formed with FACT, Nhp6, produced range...