A5066197237- Circadian rhythm and melatonin
- Photoreceptor and optogenetics research
- Genetics, Aging, and Longevity in Model Organisms
- Ubiquitin and proteasome pathways
- HER2/EGFR in Cancer Research
- Light effects on plants
- BRCA gene mutations in cancer
- CRISPR and Genetic Engineering
- Dietary Effects on Health
- Neuroblastoma Research and Treatments
- Coenzyme Q10 studies and effects
- Nutrition, Genetics, and Disease
- Genomics and Chromatin Dynamics
- 14-3-3 protein interactions
- Sleep and Wakefulness Research
- Ovarian cancer diagnosis and treatment
- Genetic factors in colorectal cancer
- Psychological and Temporal Perspectives Research
- Uterine Myomas and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Chronic Myeloid Leukemia Treatments
- Clinical Laboratory Practices and Quality Control
- RNA Research and Splicing
- Digital Imaging for Blood Diseases
- Birth, Development, and Health
Morehouse School of Medicine
2015-2023
Kaohsiung Veterans General Hospital
2019
The University of Texas MD Anderson Cancer Center
1990-2015
New York University
2005-2008
Canadian Blood Services
1999
Russian Academy of Sciences
1994
Philipps University of Marburg
1994
St. Josef-Hospital
1994
Ruhr University Bochum
1994
Robert Bosch Hospital
1994
Daily rhythms in mammals are programmed by a master clock the suprachiasmatic nucleus (SCN). The SCN contains two main compartments (shell and core), but role of each region system-level coordination remains ill defined. Herein, we use functional assay to investigate how downstream tissues interpret region-specific outputs using vivo exposure long day photoperiods temporally dissociate SCN. We then analyze resulting changes clocks located throughout brain body examine whether they maintain...
BRCA1 is the first tumor suppressor gene linked to hereditary breast and ovarian cancers. Its involvement in sporadic cancer, however, remains unclear. Recent studies showed that a loss or lowered expression of not uncommon nonfamilial In addition, there have been cases inherited BRCA1-linked cancer with as yet unidentified mutation. Misregulation at transcription level possible mechanism for expression. To understand transcriptional regulation gene, we cloned examined promoter, by both...
Circadian clocks enable organisms to predict and align their behaviors physiologies constant daily day-night environmental cycle. Because the ubiquitin ligase Siah2 has been identified as a potential regulator of circadian clock function in cultured cells, we have used SIAH2-deficient mice examine its vivo . Our experiments demonstrate striking unexpected sexually dimorphic effect SIAH2-deficiency on regulation rhythmically expressed genes liver. The absence SIAH2 females, but not males,...
The time-dependent degradation of core circadian clock proteins is essential for the proper functioning timekeeping mechanisms that drive daily rhythms in gene expression and, ultimately, an organism’s physiology. ubiquitin proteasome system plays a critical role regulating stability most proteins, including components. Our laboratory developed cell-based functional screen to identify ligases degrade any protein interest and have started screening those proteins. This identified Spsb4 as...
Amplification or overexpression both of either the c-myc human neu (C-erbB-2) gene are common events in many primary tumors. Coamplification genes have been reported some breast cancers. The possibility cooperation between and normal rat (c-neu) transforming cells was examined. Surprisingly, expression B104-1-1 cells, activated oncogene (neu*)-transformed NIH 3T3 line, resulted morphologic reversion. This reversion found to be a consequence transcription-repressive action on via 140-bp...
The proto-oncogene neu (HER2 or c-erbB2) is overexpressed with without gene amplification in 20–30% of breast cancers. In patients, overexpression and ovarian cancer correlates poor prognosis tumor resistance to chemotherapy. neu-induced transformation can be reversed by the suppression transcription. To further understand how transcription regulated identify a possible transcriptional repressor(s) ofneu, we identified negative regulatory element known previously located within 1-kilobase...
A 2.4-kb rat neu genomic DNA fragment that hybridized to the 5'-most coding sequence of cDNA was cloned. S1 nuclease mapping identified multiple transcriptional initiation sites. analysis revealed this contained 64 bp first intron, 81 exon, and upstream noncoding gene. The immediately translation start site G + C rich (greater than 75%) a consensus CCAAT despite absence TATA box. An Sp1-binding found, in addition various motifs common promoters human gene (erbB2), epidermal growth factor...
<ns4:p><ns4:bold>Background</ns4:bold>: The mammalian retina contains an autonomous circadian clock that controls many physiological functions within this tissue. Our previous studies have indicated disruption of by removing <ns4:italic>Bmal1</ns4:italic> from the affects visual function, retinal circuitry, and cone photoreceptor viability during aging. In present study, we employed a mouse-derived photoreceptor‒like cell, 661W, to investigate which molecular mechanisms may modulate...
<ns4:p><ns4:bold>Background</ns4:bold>: The mammalian retina contains an autonomous circadian clock that controls many physiological functions within this tissue. Our previous studies have indicated disruption of by removing <ns4:italic>Bmal1</ns4:italic> from the affects visual function, retinal circuitry, and cone photoreceptor viability during aging. In present study, we employed a mouse-derived photoreceptor‒like cell, 661W, to investigate which molecular mechanisms may modulate...
REV-ERBα and REV-ERBβ proteins play crucial roles in linking the circadian system to overt daily rhythms mammalian physiology behavior. In most tissues, protein robustly cycles such that it is detected only within a tight interval of 4-6 hours each day, suggesting both its synthesis degradation are tightly controlled. Several ubiquitin ligases known drive degradation, but how they interact with which lysine residues ubiquitinate promote unknown. this study, we attempted identify...
Background: Platelet clumping is a common laboratory phenomenon that causes pseudo‐thrombocytopenia (PTC), spurious thrombocytopenia due to persistence of antibody or agglutinins. Microscopic examination the peripheral blood smear key importance confirm diagnosis and avoid any inappropriate dangerous clinical therapeutic decision. How dissolve platelet make sure correct count an important procedure. Ethylenediaminetetraacetic acid (EDTA) well‐known anticoagulant for hematological tests...
Abstract Circadian clocks enable organisms to predict and align their behaviors physiologies constant daily day-night environmental cycle. Because the ubiquitin ligase Siah2 has been identified as a potential regulator of circadian clock function in cultured cells, we have used Siah2-deficient mice examine its vivo . Our experiments demonstrate striking unexpected sexually dimorphic effect deficiency on regulation rhythmically expressed genes. The absence females, but not males, altered...
Unbedingte Voraussetzung für eine Knochenmarktransplantation ist die exakte Typisierung der HLA-Merkmale von Spender und Empfänger.Wir haben Strategie HLA-DRB1-Merkmalen entwickelt, auf sequenzspezifischer Amplifikation (PCR-SSP) Oligonukleotid-Hybridisierung beruht.Aus 5 ml EDTA-Blut wird genomische DNA extrahiert, anschließend in 14 verschiedenen PCR-Ansätzen unter gleichen Reaktionsbedingungen amplifiziert wird.Dabei werden Primer spezifisch gewählt, so daß nur bestimmte Allele werden.In...