A5067959288- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Genomics and Phylogenetic Studies
- Chemical Synthesis and Analysis
- Enzyme Catalysis and Immobilization
- Synthesis and Catalytic Reactions
- Click Chemistry and Applications
- Diet, Metabolism, and Disease
- Porphyrin and Phthalocyanine Chemistry
- Photodynamic Therapy Research Studies
- Porphyrin Metabolism and Disorders
- Polysaccharides and Plant Cell Walls
- Enzyme Structure and Function
- Monoclonal and Polyclonal Antibodies Research
- Cyclopropane Reaction Mechanisms
- Biochemical Acid Research Studies
- Microbial Metabolic Engineering and Bioproduction
- Cancer Research and Treatments
- Chemical Reactions and Isotopes
- Ubiquitin and proteasome pathways
- Carbon dioxide utilization in catalysis
- Escherichia coli research studies
- Polyamine Metabolism and Applications
Axxence (Slovakia)
2024
University of Manchester
2012-2022
Czech Academy of Sciences, Institute of Biotechnology
2015-2020
Institute of Chemistry of the Slovak Academy of Sciences
2011
Slovak Academy of Sciences
2009-2011
BOKU University
2011
Philipps University of Marburg
1991
Abstract Enantiomerically pure chiral amines are ubiquitous chemical building blocks in bioactive pharmaceutical products and their synthesis from simple starting materials is of great interest. One the most attractive strategies stereoselective installation a amine through C−H amination, which challenging transformation. Herein we report application multienzyme cascade, generated single bacterial whole‐cell system, able to catalyze benzylic aminations with ee values 97.5 %. The cascade uses...
The increasing diversity of reactions mediated by biocatalysts has led to development multistep in vitro enzyme cascades, taking advantage generally compatible reaction conditions. construction pathways within single whole cell systems is much less explored, yet many advantages. Herein we report the generation a successful de novo cascade for diastereoselective and/or enantioselective conversion simple, linear keto acids into valuable cyclic amine products. pathway starts with carboxylic...
DNA-encoded libraries are increasingly used for the discovery of bioactive lead compounds in high-throughput screening programs against specific biological targets. Although a number now available, they cover limited chemical space due to bias ease synthesis and lack reactions that compatible with DNA tagging. For example, compound rarely contain complex biomolecules such as carbohydrates high levels functionality, stereochemistry, hydrophilicity. By using biocatalysis combination methods,...
Abstract Altered glycoprotein expression is an undisputed corollary of cancer development. Understanding these alterations paramount but hampered by limitations underlying cellular model systems. For instance, the intricate interactions between tumour and host cannot be adequately recapitulated in monoculture tumour-derived cell lines. More complex co-culture models usually rely on sorting procedures for proteome analyses rarely capture details protein glycosylation. Here, we report a...
O-mannosyl glycans are known to play an important role in regulating the function of α-dystroglycan (α-DG), as defective glycosylation is associated with various phenotypes congenital muscular dystrophy. Despite well-established biological significance these glycans, questions regarding their precise molecular remain unanswered. Further investigation will require synthetic methods for generation pure samples homogeneous glycopeptides diverse sequences. Here we describe first total syntheses...
Abstract Thiamine diphosphate (ThDP) dependent carboligases catalyze the formation of carbon‐carbon bonds. These enzymes are prominent biocatalysts for production valuable α‐hydroxy carbonyl compounds, which serve as key building blocks in synthesis various pharmaceuticals and fine chemicals. Carboligases act selective manner to afford regio‐ stereochemically defined products from simple starting materials. This review explores catalytic prowess synthetic purposes is aimed at providing a...
Here, we present a comparative structure–function study of nematode and plant core α1,3-fucosyltransferase based on deletion point mutations the coding regions Caenorhabditis elegans FUT-1 Arabidopsis thaliana FucTA (FUT11). In particular, our results reveal novel "first cluster motif" shared by both Lewis-type α1,3-fucosyltransferases GT10 family. To evaluate role conserved serine within this motif, residue was replaced with alanine in (S218) (S243). The S218A replacement completely...
Glycosyl phosphates are important intermediates in many metabolic pathways and substrates for diverse carbohydrate-active enzymes. Thus, there is a need to develop libraries of structurally similar analogues that can be used as selective chemical probes glycomics. Here, we explore chemoenzymatic cascades the fast generation glycosyl phosphate without protecting-group strategies. The key enzyme new bacterial galactokinase (LgGalK) cloned from Leminorella grimontii, which was produced...
The identification of carbohydrate–protein interactions is central to our understanding the roles cell–surface carbohydrates (the glycocalyx), fundamental for cell-recognition events. Therefore, there a need fast high-throughput biochemical tools capture complexity these biological interactions. Here, we describe rapid method qualitative label-free detection on arrays simple synthetic glycans, more complex natural glycosaminoglycans (GAG), and lectins/carbohydrate binding proteins using...
N-Glycolylneuraminic acid (Neu5Gc) is a common cell-surface ligand in animals which not biosynthesized humans, but it can be acquired human tissue from dietary sources such as red meat. It important to understand the relevance of this potentially immunogenic glycan on health, and selective detection methods are needed that distinguish Neu5Gc its biosynthetic precursor humans (i.e., N-acetylneuraminic (Neu5Ac)). Here, we demonstrate selectively oxidized by an engineered variant galactose...
Abstract Enantiomerenreine, chirale Amine sind unentbehrliche Bausteine in bioaktiven Pharmazeutika, und ihre Synthese aus einfachen Startmaterialien ist daher von großem Interesse. Eine der vielversprechendsten Strategien die chemisch anspruchsvolle, stereoselektive chiraler durch eine C‐H‐Aminierung. Hier berichten wir über den Einsatz einer Multienzym‐Kaskade, generiert einzelnen bakteriellen Ganzzelle, es möglich macht, Aminierungen Benzylposition mit ee ‐Werten 97.5 % zu katalysieren....
Synthesis of LacNAc with reversible GalTs.
Pseudaminic acid (Pse5Ac7Ac) is a nonmammalian sugar present on the cell surface of number bacteria including Pseudomonas aeruginosa, Campylobacter jejuni, and Acinetobacter baumannii. However, role Pse5Ac7Ac plays in host–pathogen interactions remains underexplored, particularly compared to its ubiquitous sialic analogue Neu5Ac. This primarily due lack access difficult prepare glycosides. Herein, we describe vitro biocatalytic transfer an activated donor onto glycosyl acceptors, enabling...
Within human biology, combinations of regioisomeric motifs α2,6- or α2,3-sialic acids linked to galactose are frequently observed attached glycoconjugates. These include glycoproteins and glycolipids, with each linkage carrying distinct biological information function. Microbial linkage-specific sialidases have become important tools for studying the role these sialosides in complex settings, as well being used biocatalysts glycoengineering. However, currently, there is no α2,6-specific...
To create vesicles with cell-targeting coatings, two soluble enzymes were used to directly glycosylate vesicle surfaces in a ‘one-pot’ procedure.
A new α1,4 galactosyltransferase has been characterised and used for the synthesis of natural non-natural cell surface trisaccharide antigens.
S. pneumoniae is a major human pathogen with increasing antibiotic resistance. Pneumococcal vaccines consist of capsular polysaccharide (CPS) or their related fragments conjugated to carrier protein. The repeating unit type 14 CPS shares core structure the Group B Streptococcus (GBS) III: only difference that latter exhibits sialic acid unit, α-2,3 linkage galactose. Here, automated glycan assembly (AGA) two frameshifts described. same strategy used assemble dimers different frameshifts....
Promiscuous activity of a glycosyltransferase was exploited to polymerise glucose from UDP-glucose <italic>via</italic> the generation β-1,4-glycosidic linkages.
2-Hydroxy-3-pentanone and 3-hydroxy-2-pentanone are flavor molecules present in various foods, such as cheese, wine, durian, honey, where they impart buttery, hay-like, caramel-sweet aromas. However, their utilization flavoring agents is constrained by a lack of developed synthesis methods. In this study, we from simple starting compounds available natural quality, catalyzed previously characterized ThDP-dependent carboligases. Additionally, demonstrate that newly discovered homologues...
Sialic acids are sugars present in many animal glycoproteins and of particular interest biopharmaceuticals, where a lack sialylation can reduce bioactivity. Here, we describe how α-2,6-sialyltransferase from Photobacterium damselae be used to markedly increase the level CHO-produced α-1-antitrypsin. Detailed analysis products showed that addition expected α-2,6-sialylation galactose, second disialyl galactose motif Neu5Ac-α2,3(Neu5Ac-α2,6)Gal was produced, which, our knowledge, had never...