A5071546907- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Microbial Metabolic Engineering and Bioproduction
- Microbial Metabolites in Food Biotechnology
- Diet, Metabolism, and Disease
- Enzyme Production and Characterization
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Organometallic Complex Synthesis and Catalysis
- Catalytic Cross-Coupling Reactions
- RNA regulation and disease
- Organoboron and organosilicon chemistry
- Advanced Polymer Synthesis and Characterization
- Enzyme Structure and Function
- Covalent Organic Framework Applications
- Water Treatment and Disinfection
- Skin and Cellular Biology Research
- Chemical Synthesis and Characterization
- MicroRNA in disease regulation
- Lymphatic System and Diseases
- Pharmacogenetics and Drug Metabolism
- Glycosylation and Glycoproteins Research
- Radioactive element chemistry and processing
University of Leeds
2023-2025
University of York
2015-2023
University of Oxford
2021
Oxfam
2021
Imine reductases (IREDs) catalyze the asymmetric reduction of cyclic imines, but also in some cases coupling ketones and amines to form secondary amine products an enzyme-catalyzed reductive amination (RedAm) reaction. Enzymatic RedAm reactions have typically used small hydrophobic amines, many interesting pharmaceutical targets require that larger be these reactions. Following identification IR77 from Ensifer adhaerens as a promising biocatalyst for cyclohexanone with pyrrolidine, we...
A series of 12 silver(I)–N-heterocyclic carbene (NHC) complexes were prepared featuring five- (both saturated and unsaturated backbone), six-, seven-membered ring ligand scaffolds. The N-substituents the NHCs diisopropylphenyl in all cases, while anion was varied between bromide, acetate, triflate. evaluated as catalysts spirocyclization 1-(1H-indol-3-yl)-4-phenylbut-3-yn-2-one to give a spirocyclic indolenine product. To our knowledge, it is first time that systematic study has been...
Double-stranded RNA-binding domains (dsRBDs) are commonly found in modular proteins that interact with RNA. Two varieties of dsRBD exist: canonical Type A dsRBDs dsRNA, while non-canonical B lack residues and instead other proteins. In higher eukaryotes, the microRNA biogenesis enzyme Dicer forms a 1:1 association dsRNA-binding protein (dsRBP). Human associates HIV TAR (TRBP) or activator PKR (PACT), Drosophila Dicer-1 Loquacious (Loqs). each case, interaction involves region contains dsRBD....
MicroRNAs (miRNAs) are short non-coding RNAs that silence mRNAs. They generated following transcription and cleavage by the DROSHA/DGCR8 DICER/TRBP/PACT complexes. Although it is known components of miRNA biogenesis machinery can be phosphorylated, remains poorly understood how these events become engaged during physiological cellular activation. We demonstrate S6 kinases phosphorylate extended C-terminal domain TRBP interact with in situ primary cells. serines 283/286 essential for...
Rh(<sc>i</sc>) complexes of <italic>ortho</italic>-substituted DPEphos-R (R = H, Me, OMe, <sup>i</sup>Pr) ligands show anagostic interactions; for R =<sup>i</sup>Pr C–H activation/dehydrogenative borylation forms a product exhibiting both B–H/Rh 3c-2e agostic and C–H/Rh motifs.
An amphiphilic block copolymer of polyphosphinoborane has been prepared by a mechanism-led strategy the sequential catalytic dehydropolymerization precursor monomers, H3 B ⋅ PRH2 (R=Ph, n-hexyl), using simple pre-catalyst [Rh(Ph2 PCH2 CH2 PPh2 )2 ]Cl. Speciation, mechanism and polymer chain growth studies support step-growth process where reversible transfer occurs, i.e. /oligomer/polymer can all coordinate with, be activated by, catalyst. Block [H2 BPPhH]110 -b-[H2 BP(n-hexyl)H]11...
Intravenous gallium nitrate therapy is a novel therapeutic strategy deployed to combat chronic Pseudomonas aeruginosa biofilm infections in the lungs of cystic fibrosis (CF) patients by interfering with iron (Fe 3+ ) uptake. The source Ga , which competes Fe for siderophore binding, subsequently disrupting metabolism and inhibiting proliferation vivo . It was recently demonstrated that quinolone signal (PQS) can chelate assist bacterial However, it unknown whether exogenous also targets...
A fragment screen of a library 560 commercially available fragments using kinetic assay identified small molecule that increased the activity fungal glycoside hydrolase TrBgl2. An analogue by catalogue approach and detailed analysis improved compounds behaved as nonessential activators with up to 2-fold increase in maximum activation. The did not activate related bacterial CcBglA demonstrating specificity. Interestingly, an initial inhibits both TrBgl2 CcBglA, apparently through mixed-model...
Abstract β-glucosidases have received considerable attention due to their essential role in bioethanol production from lignocellulosic biomass. β-glucosidase can hydrolyse cellobiose cellulose degradation and its low activity has been considered as one of the main limiting steps process. Large-scale conversions therefore require high enzyme concentration which increases cost. with improved thermostability are great commercial interest. The fungus Trichoderma reseei expresses thermostable...
Manuscript and SI
Manuscript and SI
The complex bis(tetrabutylammonium) (trans-μ2:η2,η2-1,3-butadiene)bis(trichloroplatinate(II)), identified from its X-ray single-crystal structure, is formed when tetrabutylammonium hexachloroplatinate(IV) irradiated in solution. In principle, the can exist one of four conformers (combining cis and trans isomers butadiene syn anti arrangements two PtCl3– moieties) DFT calculations suggest that three these ought to be stable solution CDCl3. However, dissolving anti,trans conformer (identified...
Abstract An amphiphilic block copolymer of polyphosphinoborane has been prepared by a mechanism‐led strategy the sequential catalytic dehydropolymerization precursor monomers, H 3 B ⋅ PRH 2 (R=Ph, n‐hexyl), using simple pre‐catalyst [Rh(Ph PCH CH PPh ) ]Cl. Speciation, mechanism and polymer chain growth studies support step‐growth process where reversible transfer occurs, i.e. /oligomer/polymer can all coordinate with, be activated by, catalyst. Block [H BPPhH] 110 ‐ b ‐[H BP(n‐hexyl)H] 11...