A5018474648- Congenital heart defects research
- Congenital Heart Disease Studies
- Tissue Engineering and Regenerative Medicine
- Corneal Surgery and Treatments
- Coronary Artery Anomalies
- Cardiovascular Conditions and Treatments
- Algal biology and biofuel production
- Developmental Biology and Gene Regulation
- Immune Cell Function and Interaction
- Insect-Plant Interactions and Control
- Animal Genetics and Reproduction
- Liver physiology and pathology
- Hemiptera Insect Studies
- Cardiac Fibrosis and Remodeling
- Photosynthetic Processes and Mechanisms
- TGF-β signaling in diseases
- Liver Disease Diagnosis and Treatment
- Wound Healing and Treatments
- Erythrocyte Function and Pathophysiology
- Connective tissue disorders research
- T-cell and B-cell Immunology
- Connective Tissue Growth Factor Research
- Organ Transplantation Techniques and Outcomes
- Cell Adhesion Molecules Research
- Glaucoma and retinal disorders
Osaka City University
2011-2021
Yokohama National University
2021
Rakuno Gakuen University
2018
Adult Congenital Heart Association
2014-2015
Shimane University
2014-2015
Kyoto University
2010-2013
Saitama Medical University
1996-2008
Saitama Prefectural University
2003
Mitsubishi Heavy Industries (Japan)
1997-2003
Mitsubishi Heavy Industries (Germany)
2002
Abstract The formation and transformation of the pharyngeal arch arteries in mouse embryo, from 8.5 to 13 days gestation (DG), was observed using scanning electron microscopy vascular casts graphic reconstruction 1‐µm serial epoxy‐resin sections. Late 8.5–9DG (12 somites), paired ventral aortae were connected dorsal via a loop anterior foregut which we call ‘primitive aortic arch’, as chick embryo. primitive extended cranio‐caudally be transformed into internal carotid artery, turn gave rise...
<b>Background:</b> Liver cirrhosis, which is caused by the accumulation of extracellular matrix materials, a serious clinical problem that can progress to hepatic failure. Transforming growth factor-β (TGFβ) plays pivotal role in production, but bone morphogenetic protein (BMP)-7, member TGFβ superfamily, antagonise fibrogenic activity TGFβ. <b>Aim:</b> In this study, we examined whether adenovirus-mediated overexpression BMP-7 (Ad-BMP-7) antagonised effect vitro and vivo. <b>Methods...
During early cardiac morphogenesis, outflow tract (OT) and atrio-ventricular (AV) endothelial cells differentiate into mesenchymal cells, which have characteristics of smooth muscle-like myofibroblasts, form endocardial cushion tissue, the primordia valves, septa in adult heart. this embryonic event, transforming growth factor β3 (TGFβ3) is an essential element progression endothelial-transformation mesenchyme. TGFβs are known to be a potent inducer for mesodermal differentiation promoter...
Cytoglobin/stellate cell activation-associated protein (Cygb/STAP) consists of a new class hexacoordinate globin superfamily, which was recently discovered by proteome analysis on the rat hepatic stellate cells. Unlike haemoglobin, myoglobin, and neuroglobin, Cygb/STAP is ubiquitously expressed in several organs, although its detailed localization has not been clarified. Immunohistochemistry immunoelectron microscopy revealed that uniquely localized fibroblast-like cells splanchnic namely...
Transforming growth factor-beta (TGF beta) is a dimeric peptide factor which regulates cellular differentiation and proliferation during development. Most cells secrete TGF beta as large latent complex containing mature beta, latency associated peptide, beta-binding protein (LTBP)-1. The biological role of LTBP-1 in development remains unclear. Using polyclonal antiserum specific for (Ab39) three-dimensional collagen gel culture assay embryonic heart, we examined the tissue distribution its...
Mesenchymal cells, primarily fibroblasts and myofibroblasts, are the principal matrix-producing cells during pulmonary fibrogenesis. Transforming growth factor (TGF)-β signaling plays an important role in stimulating expression of type I collagen these cells. Bone morphogenetic protein (BMP)-7, a member TGF-β superfamily, has been reported to oppose fibrogenic activity TGF-β1. Here, we have addressed effects BMP-7 on myofibroblasts. We first established cell lines from lungs transgenic mice...
Wound healing involves both local cells and inflammatory cells. Alkali burn of ocular surface tissue is a serious clinical problem often leading to permanent visual impairment resulting from ulceration, scarring neovascularization during healing. Behaviors corneal are orchestrated by growth factor signaling networks that have not been fully uncovered. Here we showed adenoviral gene introduction peroxisome proliferator-activated receptor-γ (PPARγ) inhibits activation fibroblasts macrophages...
The progress of molecular genetics has enabled us to identify the genes responsible for congenital heart malformations. However, recent studies suggest that diseases are induced not only by mutations in certain genes, but also abnormal maternal factors. A high concentration retinoic acid (RA), active derivative vitamin A, is well known as a teratogenic agent can cause developmental defects. Our previous have shown administration RA mice within narrow window induces outflow tract (OFT) septum...
We have examined the effect of adenovirus-mediated expression bone morphogenic protein-7 (BMP-7) and inhibitors differentiation 2 3 (Id2 Id3) on injury-induced epithelial-to-mesenchymal transition (EMT) lens epithelium in mice. Id2 Id3 are known to be upregulated by BMP-7 antagonize Smad2/3 signaling. The Cre-LoxP system adenoviral gene transfer was used. Three microliters solution (2 x 10(7) PFU/mul) were injected into right adult male C57BL/6 mice (n = 144) at time capsular injury induced...
Studies have shown that the proximal coronary artery (PCA) develops via endothelial ingrowth from peritruncal ring (PR) of vasculature. However, details PCA formation remain unclear. We examined development PCAs in quail embryonic hearts 5 to 9 days incubation (embryonic day [ED]) using double-immunostaining for QH1 (quail marker) and smooth muscle alpha-actin. At 6 7 ED, several QH1-positive strands PR penetrated facing sinuses, some embryos, posterior (noncoronary) sinus. 8 penetrating...