A5049991457- Spine and Intervertebral Disc Pathology
- Bone Tumor Diagnosis and Treatments
- Sarcoma Diagnosis and Treatment
- Spinal Fractures and Fixation Techniques
- Cancer-related molecular mechanisms research
- Scoliosis diagnosis and treatment
- Cervical and Thoracic Myelopathy
- MicroRNA in disease regulation
- Oral and Maxillofacial Pathology
- Medical Imaging and Analysis
- Circular RNAs in diseases
- Neurofibromatosis and Schwannoma Cases
- Infectious Diseases and Tuberculosis
- Reconstructive Surgery and Microvascular Techniques
- Musculoskeletal synovial abnormalities and treatments
- Advanced NMR Techniques and Applications
- Musculoskeletal pain and rehabilitation
- Pelvic and Acetabular Injuries
- Surgical site infection prevention
- Surgical Sutures and Adhesives
- RNA modifications and cancer
- Parkinson's Disease Mechanisms and Treatments
- Management of metastatic bone disease
- Protein Structure and Dynamics
- Spinal Cord Injury Research
Weill Cornell Medicine
2017-2024
Central South University
2015-2024
Second Xiangya Hospital of Central South University
2015-2024
Cornell University
2017-2024
Chinese Academy of Fishery Sciences
2022-2024
Jilin University
2024
Ministry of Agriculture and Rural Affairs
2022-2024
First Hospital of Jilin University
2024
Jinan University
2021-2023
Sun Yat-sen University
2017-2023
The microtubule-associated protein Tau plays a central role in the pathogenesis of Alzheimer's disease. Although interaction with membranes is thought to affect some its physiological functions and aggregation properties, sequence determinants structural functional consequences such interactions remain poorly understood. Here, we report that vesicles results formation highly stable protein/phospholipid complexes. These complexes are toxic primary hippocampal cultures detected by MC-1, an...
A strategy for simplified and complete resonance assignment of insoluble noncrystalline proteins by solid-state NMR (ssNMR) spectroscopy is presented. Proteins produced with [1-13C]- or [2-13C]glucose are very sparsely labeled, the resulting 2D ssNMR spectra exhibit smaller line widths (by a factor ∼2 relative to uniformly labeled proteins) contain reduced number cross-peaks. This allows an accelerated straightforward without necessity time-consuming 3D sophisticated pulse sequences. The...
Abstract The inflammatory microenvironment in the joints is one of critical issues during osteoarthritis (OA) and also main factor that may aggravate symptoms. Under microenvironment, M1 macrophages are activated produce large numbers proinflammatory mediators, leading to production degradative enzymes, disturbance chondrocyte apoptosis cartilage catabolic processes, finally deterioration OA. In present study, we reveal overexpression osteopontin (OPN), a cytokine, matrix protein involved...
Abstract Background With cancer-associated fibroblasts (CAFs) as the main cell type, rich myxoid stromal components in chordoma tissues may likely contribute to its development and progression. Methods Single-cell RNA sequencing (scRNA-seq), spatial transcriptomics, bulk RNA-seq, multiplexed quantitative immunofluorescence (QIF) were used dissect heterogeneity, distribution, clinical implication of CAFs chordoma. Results We sequenced here 72 097 single cells from 3 primary recurrent tumor...
ABSTRACT During the process of Intervertebral disc degeneration (IDD), nucleus pulposus apoptosis increases, extracellular matrix (ECM) alters and/or degrades, abnormal proliferation cells forms cell clusters, and expression various inflammatory factors increases. Thus, regulation human (HNPC) ECM synthesis present promising strategies for IDD treatment. Accumulating evidence indicates that non‐coding RNAs are involved in cellular processes, including proliferation, differentiation,...
MicroRNA (miR) plays an important role in tumorigenesis including malignant peripheral nerve sheath tumor (MPNST). miR-210 downregulation is frequently observed a variety of tumors. In this study, was identified as downregulated MPNST cells, and its potential target ephrin-A3 (EFNA3) upregulated them compared with neurofibroma cells using quantitative real-time (qRT)-PCR. Luciferase reporter assay further demonstrates that EFNA3 miR-210. Then it confirmed can regulate mRNA protein expression...
Osteosarcoma (OS) is the most common primary bone malignancy with a poor prognosis for all races and both sexes. In this study, we found that miR-381 positive factor OS patients low expression of had longer survival time after surgical intervention, promotes MG-63 cell proliferation invasion ability. Our results also showed strong negative correlation between LRRC4 (brain relative specific gene) in tissues. This demonstrated direct target gene miR-381, suppressing increases sensitivity cells...
Currently, clinical implications of immune system cells in chordoma remain to be elucidated.To characterize situ cell infiltrates, the Immunoscore, and investigate their correlation with clinicopathologic data spinal patients outcome.Tumor-infiltrating lymphocytes (TILs) subtypes were assessed 54 tumor specimens using immunohistochemistry for CD3, CD4, CD8, CD20, Foxp3, PD-1, PD-L1.Overall, infiltrates present all samples there was low or moderate among several TILs subsets. PD-1+ density,...
The present study aimed to explore the underlying mechanisms of bone morphogenetic protein 2 (BMP2) in alleviating intervertebral disc degeneration (IDD). A rat puncture IDD model was constructed, and rats were randomly divided into six groups: Control; (model); IDD+PBS [containing 1010 adeno‑associated virus serotype (AAV)]; + AAV2‑BMP2 (106, 108 1010). IL‑1β used treat primary nucleus pulposus (NP) cells mimic vitro. effects BMP2 determined by magnetic resonance imaging (MRI), hematoxylin...
Currently, little is known about the clinical relevance of tumor-stroma ratio (TSR) in chordoma and data discussing relationship between TSR immune status are lacking.To characterize distribution spinal chordoma, investigated its correlation with clinicopathologic or immunological features patients outcome.TSR was assessed visually on hematoxylin eosin-stained sections from 54 tumor specimens by 2 independent pathologists. Multiplex immunofluorescence used to quantify expression levels...
Exosomes (exo) are involved in the progression of osteoarthritis (OA). This study aimed to investigate function dysfunctional chondrocyte-derived exo (DC-exo) on OA rats and rat macrophages. Rat-derived chondrocytes were isolated, DCs induced with interleukin (IL)-1β used for isolation. Rats (n = 36) or macrophages treated DC-exo phosphate-buffered saline (PBS). Macrophage polarization autophagy, degradation chondrocyte activity cartilage tissues, examined. RNA sequencing was detect genes...