A5083361489- Developmental Biology and Gene Regulation
- Genomics and Chromatin Dynamics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA Research and Splicing
- Animal Genetics and Reproduction
- Invertebrate Immune Response Mechanisms
- Chromosomal and Genetic Variations
- Epigenetics and DNA Methylation
- Neurobiology and Insect Physiology Research
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Pluripotent Stem Cells Research
- Insect and Arachnid Ecology and Behavior
- RNA Interference and Gene Delivery
- Plant Molecular Biology Research
- Wnt/β-catenin signaling in development and cancer
- Monoclonal and Polyclonal Antibodies Research
- Genetics and Physical Performance
- RNA modifications and cancer
- Sperm and Testicular Function
- Glycosylation and Glycoproteins Research
- Hippo pathway signaling and YAP/TAZ
- Plant Reproductive Biology
- Sexual Differentiation and Disorders
Indian Institute of Science Education and Research Pune
2017-2024
Princeton University
2015-2024
Illinois College
2022
John Brown University
2021
Brown University
2021
Indian Institute of Science Education and Research Berhampur
2018
Indian Institute of Science Education and Research Mohali
2018
Indian Institute of Science Education and Research Kolkata
2018
Indian Institute of Science Education and Research, Bhopal
2018
Indian Institute of Science Education and Research, Tirupati
2018
During the essential and conserved process of zygotic genome activation (ZGA), chromatin accessibility must increase to promote transcription. Drosophila is a well-established model for defining mechanisms that drive ZGA. Zelda (ZLD) key pioneer transcription factor (TF) promotes ZGA in embryo. However, many genomic loci contain GA-rich motifs become accessible during independent ZLD. Therefore, we hypothesized other early TFs function with ZLD have not yet been identified, especially those...
The RNA-induced silencing complex (RISC) or the RISC mediates RNAi and is comprised of proteins belonging to dicer Argonaute family proteins. Here we show that Argonaute-2 ( ago-2 ) required for proper nuclear migration, pole cell formation, cellularization during early stages embryonic development in Drosophila . We have traced these defects back division cycles. Unlike wild type, asynchronous embryos there are chromosome condensation, kinesis, assembly spindle apparatus. aberrations cycle...
Insulators play a central role in gene regulation, chromosomal architecture and genome function higher eukaryotes. To learn more about how insulators carry out their diverse functions, we have begun an analysis of the Drosophila CTCF (dCTCF). is one few insulator proteins known to be conserved from flies man. In studies reported here focused on identification characterization two dCTCF protein interaction modules. The first mediates multimerization, while second dCTCF–CP190 interactions....
Chromatin boundaries are architectural elements that determine the three-dimensional folding of chromatin fiber and organize chromosome into independent units genetic activity. The Fab-7 boundary from Drosophila bithorax complex (BX-C) is required for parasegment-specific expression Abd-B gene. We have used a replacement strategy to identify sequences necessary sufficient function in BX-C. activity known depend on factors stage specific, we describe novel ∼700-kDa complex, late (LBC), binds...
Functionally autonomous regulatory domains direct the parasegment-specific expression of Drosophila Bithorax complex (BX-C) homeotic genes. Autonomy is conferred by boundary/insulator elements that separate each domain from its neighbors. For six nine parasegment (PS) in complex, at least one boundary located between and target gene. Consequently, BX-C boundaries must not only block adventitious interactions neighboring domains, but also be permissive (bypass) for their gene targets. To...
The primordial germ cells (PGCs) specified during embryogenesis serve as progenitors to the adult germline stem cells. In Drosophila, proper specification and formation of PGCs require both centrosomes plasm, which contains determinants. Centrosomes are microtubule (MT)-organizing centers that ensure faithful segregation plasm into PGCs. To date, mechanisms modulate centrosome behavior engineer PGC development have remained elusive. Only one component, Germ cell-less (Gcl), is known play a...
In Drosophila melanogaster embryos, somatic versus germline identity is the first cell fate decision. Zygotic genome activation (ZGA) orchestrates regionalized gene expression, imparting specific on cells. ZGA begins with a minor wave that commences at nuclear cycle (NC)8 under guidance of chromatin accessibility factors (Zelda, CLAMP, GAF), followed by major during NC14. By contrast, primordial germ (PGC) specification requires maternally deposited and posteriorly anchored determinants....
In Drosophila melanogaster, the germline precursor cells, i.e. pole are formed at posterior of embryo. As observed for newly germ cells in many other eukaryotes, distinguished from soma by their transcriptional quiescence. To learn more about mechanisms involved establishing quiescence, we ectopically expressed a potent activator, Bicoid (Bcd), cells. We find that Bcd overrides machinery downregulates transcription, and activates not only its target gene hunchback but also normally female...
Abstract fl(2)d, the Drosophila homolog of Wilms'-tumor-1-associated protein (WTAP), regulates alternative splicing Sex-lethal (Sxl), transformer (tra), and Ultrabithorax (Ubx). Although WTAP has been found in functional human spliceosomes, exactly how it contributes to process remains unknown. Here we attempt identify factors that interact genetically physically with fl(2)d. We begin by analyzing Sxl-Fl(2)d protein–protein interaction detail present evidence suggesting female-specific...
A critical step in animal development is the specification of primordial germ cells (PGCs), precursors germline. Two seemingly mutually exclusive mechanisms are implemented across kingdom: epigenesis and preformation. In epigenesis, PGC non-autonomous depends on extrinsic signaling pathways. The BMP pathway provides key signals mammals. Preformation autonomous mediated by determinants localized within PGCs. Drosophila , a classic example preformation, constituents plasm at embryonic...
The primary sex determination signal, the X chromosome-to-autosome (X/A) ratio, controls choice of sexual identity in Drosophila melanogaster embryo by regulating activity early promoter Sex-lethal gene, Sxl-Pe. This is activated females (2X/2A), while it remains off males (1X/2A). Promoter activation dependent upon X-linked numerator genes. One these genes, sisterless-b (sis-b), corresponds to scute (sc) locus achaete-scute complex, and encodes a helix-loop-helix transcription factor. In...
Intercellular communication mediated by gap junction (GJ) proteins is indispensable during embryogenesis, tissue regeneration and wound healing. Here we report functional analysis of a protein, Innexin 2 (Inx2), in cell type specification Drosophila oogenesis. Our data reveal novel involvement Inx2 the Border Cells (BCs), migratory type, whose identity determined autonomous STAT activity. We show that influences BC fate modulating activity via Domeless receptor endocytosis. Furthermore,...
In Drosophila melanogaster, Sex-lethal (Sxl) controls autoregulation and sexual differentiation by alternative splicing but regulates dosage compensation translational repression. To elucidate how Sxl functions in regulation, we have ectopically expressed a full-length protein (Sx.FL) lacking the N-terminal 40 amino acids (Sx-N). The Sx.FL recapitulates activity of gain-of-function mutations, as it is both sex transforming lethal males. contrast, Sx-N unlinks sex-transforming male-lethal...
The FMR family of KH domain RNA-binding proteins is conserved from invertebrates to humans. In humans, inactivation the X-linked gene fragile X most common cause mental retardation and leads defects in neuronal architecture. While there are three members only a single gene, dfmr1, flies. As dfmr1 causes architecture behavior. has other functions fly addition neurogenesis. Here we have analyzed its role during early embryonic development. We found that embryos display rapid nuclear division...
In female fruit flies, Sex-lethal (Sxl) turns off the X chromosome dosage compensation system by a mechanism involving combination of alternative splicing and translational repression male specific lethal-2 (msl-2) mRNA. A genetic screen identified translation initiation factor eif4e as gene that acts together with Sxl to repress expression Msl-2 protein. However, is not required for mediated msl-2 mRNA translation. Instead, functions co-factor in Sxl-dependent female-specific also...
Abstract The embryonic gonad of Drosophila melanogaster begins to display sexually dimorphic traits soon after its formation. Here we demonstrate the involvement a wnt family ligand, wnt-2, in induction these sex-specific differences. We show that wnt-2 contributes survival male-specific population somatic gonadal precursor cells (SGPs), SGPs are located at posterior male gonad. also Wnt-2 ligand synergizes with JAK-STAT Upd, which is produced by anterior activate STAT pathway germ cells....
Abstract Zygotic genome activation (ZGA) initiates regionalized transcription underlying distinct cellular identities. ZGA is dependent upon dynamic chromatin architecture sculpted by conserved DNA-binding proteins. However, the direct mechanistic link between onset of and tissue-specific remains unclear. Here, we have addressed involvement organizer Satb2 in orchestrating both processes during zebrafish embryogenesis. Integrative analysis transcriptome, genome-wide occupancy accessibility...
Embryonic patterning is critically dependent on zygotic genome activation (ZGA). In Drosophila melanogaster embryos, the pioneer factor Zelda directs ZGA, possibly in conjunction with other factors. Here, we have explored novel involvement of Chromatin-Linked Adapter for MSL Proteins (CLAMP) during ZGA. CLAMP binds thousands sites genome-wide throughout early embryogenesis. Interestingly, relocates to target promoter sequences across when ZGA initiated. Although there a considerable overlap...
The Drosophila sex determination gene Sex-lethal controls its own expression and the of downstream target genes such as transformer by regulating RNA splicing. Genetic molecular studies have established that Sxl requires product another gene, snf, to autoregulate splicing transcripts. snf has recently been shown encode a U1 U2 small nuclear ribonucleoprotein particle protein. In work reported here, we demonstrate Snf proteins can interact directly in vitro these two are part an...
The specification of primordial germ cells (PGCs) and subsequent maintenance germ-line identity in Drosophila embryos has long been thought to occur solely under the control cell-autonomous factors deposited posterior pole plasm during oogenesis. However, here we document a novel role for somatic BMP signaling PGC fate period leading up embryonic gonad coalescence. We find that PGCs fail maintain their germline when is compromised. They initiate but are unable properly assemble stem...