ABSTRACT Of the 181 unduplicated Escherichia coli strains isolated in nine different hospitals three Portuguese regions, 119 were extended-spectrum β-lactamase (ESBL)-CTX-M producers and selected for phenotype genotype characterization. CTX-M producer prevalent among community-acquired infections (56%), urinary tract (76%), patients ≥60 years old (76%). In MIC tests, all resistant to cefotaxime, 92% ceftazidime, 93% quinolones, 89% aminoglycoside, 26% trimethoprim-sulfamethoxazole; sensitive...

Background and aim During endovascular procedures for acute ischemic stroke, catheter access to the occluded vessel may be technically difficult or impossible. The of this study was impact target carotid artery on clinical outcome. Methods Anterior circulation stroke patients undergoing transfemoral where studied. Patients were divided into four groups according time from groin puncture catheterization quartiles. in quartile 4 (Q4) considered access. We defined several outcome measures:...

Salmonella enterica is a zoonotic foodborne pathogen that causes acute gastroenteritis in humans. We assessed the virulence potential of one-hundred and six strains isolated from food animals products. A high through-put genes microarray demonstrated Pathogenicity Islands (SPI) adherence were highly conserved, while prophages plasmid variably present. Isolates grouped by serotype, plasmids separated S. Typhimurium two clusters. Atypical results lead to whole genome sequencing (WGS) Infantis...

ABSTRACT BACKGROUND AND PURPOSE Recent reports have indicated that mechanical thrombectomy may potential to treat acute ischemic stroke. However, few comparative studies of neurothrombectomy devices are reported. This study aims compare the safety and effectiveness two retrievable stent systems in stroke patients. METHODS A prospective comparing clinical, radiological, functional outcome 33 patients with an angiographically verified occlusion anterior cerebral circulation. Patients were...

Introduction: The main objective of the study was molecular characterization extended spectrum β-lactamases (ESBL) in Escherichia coli isolates collected from human and bovine samples Oyo state, Nigeria. Methodology: Between August 2010-2011, 114 E. were hospitals (n = 57) 57). PCR sequencing used for identification ESBLs, upstream sequences, plasmid-mediated quinolone resistance (PMQR) genes class 1 integrons. Plasmid incompatibility groups identified among ESBL-positive by PCR. Genetic...

This study aimed to characterize the relationship between pathogenicity islands (PAIs), single virulence genes and resistance among uropathogenic Escherichia coli, evaluating plasmid carriage fitness cost related PAIs. For 65 urinary E. antimicrobial susceptibility extended-spectrum β-lactamase production were determined with Vitek 2 Advanced Expert system. Phylogroup determination, detection of PAIs papAH, papC, sfa/foc, afa/dra, iutA, kpsMII, cnf1, eaeA, hlyA, stx1 stx2, replicon typing...

Information on the clinical and hemodynamic profile of intravenous tissue-type plasminogen activator nonresponders, at different locations arterial occlusion, may improve selection candidates for rescue reperfusion therapies. Therefore, we aim to investigate predictors failing therapy according occluded vessel location clot.We prospectively evaluated consecutive patients with an acute ischemic stroke admitted within first 6 hours onset. Five hundred forty-eight documented intracranial...

As Salmonella is a common foodborne pathogen, the present study aimed to determine distribution of enterica serotypes isolated during 2011–2012 from poultry, swine, cattle, and processed food in Portugal, characterize antimicrobial susceptibility extended-spectrum β-lactamases (ESBLs). Results were also compared with data obtained before implementation National Control Program Poultry ban agents animal feed European Union (EU). A total 14 identified, 258 isolates recovered, Typhimurium...

Background: Globally, and particularly in developing countries, the menace of anti-microbial resistance is an accelerating problem. In Nigeria, increase bacterial has been phenotypically established but due to high cost, few molecular studies have reported.Objectives: This study screened for presence transferable genes mobile genetic elements (MGEs) such as integron among multi-drug resistant (MDR) P. mirabilis.Methods: A total 108 mirabilis strains collected from five tertiary hospitals...

ABSTRACT Sixteen strains of Escherichia coli isolated between January and June 2005 in a hospital Algiers carry the IS Ecp1 element TEM either CTX-M-3 ( n = 3) or CTX-M-15 13) β-lactamases. Fourteen isolates are multidrug resistant. Five from neonatal ward were indistinguishable by pulsed-field gel electrophoresis.

ABSTRACT BACKGROUND AND PURPOSE Recent reports have indicated that mechanical thrombectomy may the potential to treat acute ischemic stroke. This study aims describe safety and effectiveness of Trevo Retriever, using Stentriever technology, in revascularization patients with METHODS Prospective evaluating clinical, radiological, functional outcome 13 an angiographically verified occlusion anterior cerebral circulation. All underwent TR as monotherapy or combination intra‐arterial...

Escherichia coli ST131 is an important cause of multidrug-resistant infections. Thus, the aim this study was to evaluate concomitant presence resistance plasmids and pathogenicity islands (PAIs) in E. coli. From 97 extraintestinal characterized for antimicrobial susceptibility extended-spectrum β-lactamase production, 16% isolates were identified as CTX-M-15 producers. These strains studied by PFGE, MLST, phylogroups, plasmid groups, PAIs, plasmid-mediated quinolone-resistance determinants....

Salmonella enterica is a foodborne pathogen showing increasing multidrug resistance (MDR). We characterized the antimicrobial (AMR) genotype using microarrays in panel of 105 nontyphoidal S. isolated from food animals and foodstuff. Nineteen isolates were chosen on basis their MDR virulence for determination heavy metal susceptibilities screened by polymerase chain reaction genes. Whole-genome sequencing (WGS) was performed three carrying clinically important AMR genes cdtB toxin gene to...

Escherichia coli is simultaneously an indicator of water contamination and a human pathogen. This study aimed to characterize the virulence resistance E. from municipal hospital wastewater treatment plants (WWTPs) in central Portugal. From total 193 isolates showing reduced susceptibility cefotaxime and/or nalidixic acid, 20 with genetically distinct fingerprint profiles were selected characterized. Resistance antimicrobials was determined using disc diffusion method. Extended spectrum...

The presence of antimicrobial resistance and virulence factors 174 Escherichia coli strains isolated from healthy Portuguese Gallus gallus was evaluated. Resistance profiles were determined against 33 antimicrobials by microbroth dilution. prevalent for tetracycline (70%) ampicillin (63%). Extended-spectrum beta-lactamase (ESBL) phenotype observed in 18% the isolates. Multidrug found 56% A subset 74 isolates screened DNA microarrays carriage 88 antibiotic genes 62 genes. Overall, 37...

ABSTRACT The new β-lactamase SHV-72 was isolated from clinical Klebsiella pneumoniae INSRA1229, which exhibited the unusual association of resistance to amoxicillin-clavulanic acid combination (MIC, 64 μg/ml) and susceptibility cephalosporins, aztreonam, imipenem. (pI 7.6) harbored three amino substitutions Ile8Phe, Ala146Val, Lys234Arg. had high catalytic efficiency against penicillins ( k cat / K m , 35 287 μM −1 ·s ) no activity oxyimino β-lactams. concentration clavulanic necessary...

Introduction: Dural arteriovenous fistulas (DAVFs) are important causes of neurological dysfunction and many times misdiagnosed. Particularly in older populations, DAVFs may present with a selective cognitive dysfunction. Case Report: The authors describe 70-year-old woman presenting rapidly progressive dementia, very similar presentation to prion disease. Neuroimaging showed multifocal associated venous thrombosis white matter changes, suggesting that impaired cerebral circulation due...

Abstract The aims of this article were to explore the relationship between depressive symptoms and neuropsychological performance in a sample HIV-infected women, examine contribution demographic, HIV-related variables, neurocognitive performance. In cross-sectional study, 103 recruited from February December 2010, assessed for (with Beck Depression Inventory) HIV Dementia Scale). Severe reported by 31.1% women. Findings indicated that severe levels significantly associated with reduced...