A5077101029- Listeria monocytogenes in Food Safety
- Food Safety and Hygiene
- Phytochemicals and Antioxidant Activities
- Viral gastroenteritis research and epidemiology
- Salmonella and Campylobacter epidemiology
- Essential Oils and Antimicrobial Activity
- Bee Products Chemical Analysis
- Wound Healing and Treatments
- Clostridium difficile and Clostridium perfringens research
- Meat and Animal Product Quality
- Zoonotic diseases and public health
- Yersinia bacterium, plague, ectoparasites research
- Edible Oils Quality and Analysis
- Antibiotic Resistance in Bacteria
- Probiotics and Fermented Foods
- Pharmacological Effects of Natural Compounds
- Identification and Quantification in Food
- Electrospun Nanofibers in Biomedical Applications
- Bacillus and Francisella bacterial research
- Advancements in Transdermal Drug Delivery
- Microbial Inactivation Methods
- Polysaccharides Composition and Applications
- Saffron Plant Research Studies
- Microencapsulation and Drying Processes
- Food Security and Health in Diverse Populations
Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche
2015-2025
Campylobacteriosis, a prevalent foodborne gastrointestinal infection in Europe, is primarily caused by Campylobacter jejuni and coli , with rising global concerns over antimicrobial resistance these species. This study comprehensively investigates 133 human-origin spp. strains (102 C. 31 ) collected Italy from 2013 to 2021. The predominant Multilocus Sequence Typing Clonal complexes (CCs) were ST-21 CC ST-206 ST-828 . Ciprofloxacin tetracycline resistance, mainly attributed GyrA (T86I)...
On August 28, 2015, a staphylococcal food poisoning outbreak occurred in Umbria, Italy, affecting 24 of the 42 customers who had dinner at local restaurant. About 3 h after ingesting variety foods, manifested gastrointestinal symptoms. Within notification from hospital emergency department, Sanitary Inspectors Public Health Unit performed an epidemiological investigation. A retrospective cohort study was conducted among customers. Food and environmental samples were collected. Due to rapid...
Hazelnut shells represent a waste material (about 42% of the total biomass) deriving from hazelnut harvest. These are mainly used as heating source; however, they an interesting source polyphenols useful in health field. The impact on phenolic profile and concentrations shell extracts obtained by three extraction methods (maceration, ultrasonic bath, high-power ultrasonic), well temperature, time, preventive maceration, was studied. prepared were characterized terms chemical composition,...
Pycnogenol (PYC) is a concentrate of phenolic compounds derived from French maritime pine; its biological activity as antioxidant, anti-inflammatory and antibacterial suggests use in the treatment open wounds. A bioadhesive film, loaded with PYC, was prepared by casting, starting combination two biopolymer acqueous solutions: xanthan gum (1% wt/wt) sodium alginate (1.5% wt/wt), 2.5/7.5 (wt/wt) ratio. In both solutions, glycerol (10% added plasticizing agent. The film resulted an adhesive...
Despite the technologies applied to food production, microbial contamination and chemical deterioration are still matters of great concern. In order limit these phenomena, new natural approaches should be applied. this context, present study aimed assess antioxidant anti-Clostridial effects two different polyphenolic extracts derived from olive mill vegetation water, one liquid (LE) encapsulated (EE). The have been preliminary characterized using Liquid Chromatography Quadrupole Time-Of...
The emergence of Clostridioides difficile as the main agent antibiotic-associated diarrhoea has raised concerns about its potential zoonotic role in different animal species. use antimicrobials is a major risk factor for C. infection. Here, we provide data on infection dairy and beef calves Umbria, region central Italy. This cross-sectional study focuses prevalence, factors, ribotypes, toxinotypes antimicrobial resistance profiles circulating ribotypes. A prevalence 19.8% (CI95%, 12–27.6%)...
Listeria monocytogenes (LM) is a frequent post-process contaminant in meat products. This study aimed to investigate the antilisterial effectiveness of antimicrobial treatments employing olive mill wastewater polyphenolic extract (PE) commercially manufactured frankfurters. Frankfurters were inoculated on surface with three-strain LM mixture (~102 CFU/g), treated PE 2-fold series concentrations (PM, 2PM, 4PM) and control group (CTR) PE-untreated samples. Then, frankfurters vacuum-packed...
Game meat production strongly differs from that of other meats, as peculiar factors present in the field and steps prior to transfer a game-handling establishment can influence hygiene carcasses and, therefore, meat. The effects such were considered hunted wild boars based on main hygienic criteria adopted processing. Environmental, animal, hunting conditions studied during two selective seasons Central Italy. A total 120 boar sampled after skinning process analyzed for aerobic colony count,...
The success of wound treatment is conditioned by the combination both suitable active ingredients and formulation. Grape seed extract (GSE), a waste by-product obtained grape processing, natural source rich in many phenolic compounds responsible for antioxidant, anti-inflammatory, antimicrobial activities this reason useful to be used care product. Bioadhesive polymeric patches have been realized combining acacia gum (AG) polyvinylpyrrolidone (PVP). Prototypes were prepared considering...
Consumption of raw or undercooked wild boar (WB) meat is considered an important risk factor for hepatitis E virus (HEV) infection in humans. The possibility HEV contamination during the slaughtering practices may pose additional risk. Based on these assumptions, we evaluated WB hunted Umbria (central Italy) 2022–2023 hunting season by real-time RT-PCR. Herein, show that 10.8% livers from slaughtered were positive RNA, thus providing estimate region. Then, evaluating paired liver–muscle...
The mincing process of raw meat favors microbial spoilage as well chemical and enzymatic oxidation processes. In order to limit this degradative process, preservatives are routinely added minced products. role olive mill wastewater polyphenolic extract a replacement for synthetic in beef burger was assessed. antioxidant capacity the experimentally evaluated using oxygen radical absorbance method (ORACFL) assess shelf-life, while lipid measured by thiobarbituric reactive substance (TBAR)...
In recent years, there has been a growing interest in the use of novel antimicrobial agents able to inhibit or kill food-borne bacteria interrupt onset food spoilage. Crocus sativus L. petals, typically considered as waste obtained from saffron spice production, could be source natural bioactive compounds used preservatives. The purpose this work was investigate antibacterial properties two hydroalcoholicsaffron petal extracts by maceration (SPEA) and ultrasonic bath (SPEB) methods. main...
Dry (D.E.) and liquid (L.E.) extracts were prepared from flaxseeds their application in health field was evaluated. The chemical analysis showed that D.E. is rich the lignan secoisolariciresinol diglucoside L.E. unsaturated triglycerides containing linolenic acid. Mainly, reducing (15.73 μmol Fe2+/g) radical scavenging capacities (5.25 mg TE/g) ability to down-regulate expression of pro-inflammatory cytokines NO (IC50 = 0.136 ± 0.009 mg/mL) IL-6 0.308 0.103 mg/mL), suggesting its use wound...
A survey was conducted from 2018 to 2023 assess the presence of Salmonella in 280 hunted wild boar (carcasses after evisceration and skinning, N = 226; liver, 258; fecal samples, 174). The overall prevalence 2.86% (confidence interval 95%, 1.45–5.45%) with five positive samples detected carcasses, three one a sample. This line those found nearby areas denoting low number samples. Positive animals were over 24 months age weighed, before 59.00 ± 9.11 Kg no difference microbial loads between...
Snail slime is an interesting material for effective dermatological use (e.g. wounds). Its properties are stricly connected to the origin. In this paper a snail slime, deriving from species Helix aspersa Muller and obtained company, was deeply characterized then properly formulated. The by Donatella Veroni method, firstly submitted NMR analysis in order evaluate chemical composition. main molecules found glycolate allantoin, well known their activities wound healing promotion. vitro...
This study investigates the contamination of foods with Clostridium difficile in 3 hospitals Central Italy. We used real-time PCR for tpi gene to analyse 350 food samples, including 296 cooked meals and 54 be eaten raw. The molecular screening test was positive but toxigenic C. isolated only two them. prevalence 0,3% (1/296), while uncooked processed 1,9% (1/54). Data support potential risk as a source hospitalized patients, further investigations are needed.
In the last decade, incidence and severity of Clostridioides difficile infections (CDIs) in humans have been increasing community-associated described. For these reasons, interest C. food animals has increased, suggesting other possible sources acquisition. This study evaluated presence on pig carcasses at slaughterhouse pork products Central Italy. The contamination rate was 4/179 (2.3%). Regarding samples, a total 216 were tested (74 raw meat preparations 142 ready-to-eat samples made by...
The Arcobacter genus comprises a group of bacteria widely distributed in different habitats that can be spread throughout the food chain. Fluoroquinolones and aminoglycosides represent most common antimicrobial agents used for treatment infections. However, increasing trend resistance this pathogen leads to failures. Moreover, test implementation interpretation are hindered by lack reference protocols standard interpretive criteria. purpose our study was assess antibiotic pattern 17 A....
Food products are prone to microbial contamination able affect their safety and quality attributes nutritional value. The interest in the potential use of bioactive compounds deriving from natural matrices, especially agro-industrial wastes, as alternatives classical food preservatives has rapidly increased. In present study a grade olive mill wastewater polyphenolic extract commercial mix were characterised antioxidant antimicrobial capacity assessed. activity was preliminary assessed vitro...
The wild boar (Sus scrofa) population in central Italy has strongly increased the last decades. meat of game is characterized by high-quality value and manufacture food products from could represent a remarkable added for local market promoting gastronomic specialties traditions. Adult animals were hunted with waiting method carcasses processed into processing center. Five batches salami produced different amounts pork meat. microbiological, physicochemical, rheological, sensory evaluations...