A5041092558- DNA and Nucleic Acid Chemistry
- DNA Repair Mechanisms
- Photodynamic Therapy Research Studies
- Synthesis and Characterization of Heterocyclic Compounds
- Advanced biosensing and bioanalysis techniques
- Chemical Synthesis and Analysis
- Crystallization and Solubility Studies
- Nanoplatforms for cancer theranostics
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- X-ray Diffraction in Crystallography
- Rock Mechanics and Modeling
- Biochemical and Molecular Research
- Insect symbiosis and bacterial influences
- Synthesis and biological activity
- RNA Interference and Gene Delivery
- Skin Protection and Aging
- Photochromic and Fluorescence Chemistry
- Photochemistry and Electron Transfer Studies
- Synthesis and Biological Evaluation
- SARS-CoV-2 and COVID-19 Research
- Click Chemistry and Applications
- Catalytic Processes in Materials Science
- HIV/AIDS drug development and treatment
- Porphyrin and Phthalocyanine Chemistry
Chongqing University
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
Peking Union Medical College Hospital
2025
University College London
1995-2024
The Open University
2014-2024
Sichuan University
2013-2024
Johns Hopkins University
2021-2024
Johns Hopkins Medicine
2021-2024
Donghua University
2022-2024
State Key Laboratory for Modification of Chemical Fibers and Polymer Materials
2022-2024
Genetic strategies that reduce or block pathogen transmission by mosquitoes have been proposed as a means of augmenting current control measures to the growing burden vector-borne diseases. The endosymbiotic bacterium Wolbachia has long promoted potential vehicle for introducing disease-resistance genes into mosquitoes, thereby making them refractory human pathogens they transmit. Given large overlap in tissue distribution and intracellular localization between dengue virus we conducted...
Oxidative stress and mutagenic DNA lesions formed by reactive oxygen species (ROS) are linked to human malignancy. Clinical treatments inducing chronic oxidative may therefore carry a risk of therapy-related cancer. We suggest that immunosuppression azathioprine (Aza) be one such treatment. Aza causes the accumulation 6-thioguanine (6-TG) in patients' DNA. Here we demonstrate biologically relevant doses ultraviolet A (UVA) generate ROS cultured cells with 6-TG-substituted 6-TG UVA...
Wolbachia is a maternally transmitted symbiotic bacterium of insects that has been proposed as potential agent for the control insect-transmitted diseases. One major limitations preventing development malaria inability to establish inherited infections in anopheline mosquitoes. Here, we report establishment stable infection an important vector, Anopheles stephensi. In A. stephensi, strain wAlbB displays both perfect maternal transmission and ability induce high levels cytoplasmic...
It is proposed here that the delayed cytotoxicity of thioguanine involves postreplicative DNA mismatch repair system. After incorporation into DNA, chemically methylated by S -adenosylmethionine to form 6 -methylthioguanine. During replication, -methylthioguanine directs either thymine or cytosine growing strand, and resultant -methylthioguanine-thymine pairs are recognized Azathioprine, an immunosuppressant used in organ transplantation, partly converted thioguanine. Because carcinogenicity...
The development of nanomedicine formulations to overcome the disadvantages traditional chemotherapeutic drugs and integrate cooperative theranostic modes still remains challenging. Herein, we report facile construction a multifunctional nanoplatform based on doxorubicin (DOX)-loaded tannic acid (TA)-iron (Fe) networks (for short, TAF) coated with fibronectin (FN) for combination tumor chemo-/chemodynamic/immune therapy under guidance magnetic resonance (MR) imaging. We show that DOX-TAF@FN...
Polylactide (PLA), a biobased polymer, has short elongation at break and low impact strength, which restricted its broad application as commodity polymer. In this paper, super-tough polylactide/crosslinked polyurethane (PLA/CPU) binary blends with CPU dispersed in the PLA matrix were prepared by reactive blending of poly(ethylene glycol) (PEG) polymeric methylene diphenylene diisocyanate (PMDI). The situ polymerization PEG PMDI formed CPU, interfacial compatibilization between phases...
O<sub>act</sub>: active oxygen species. C*[O]: carbon–oxygen intermediate. The synergistic effect in Co–Ce catalyst leads to more vacancies and enhanced redox ability, thus assisting soot combustion.
This prospective randomized study compared the outcome of elderly patients with an unstable pertrochanteric fracture, treated a proximal femoral nail antirotation device (PFNA; n = 51) or dynamic hip screw (DHS; 55). All in DHS group and nine PFNA had open reductions. Incisions were significantly shorter for than group. Blood loss number requiring postoperative blood transfusions greater, but operation fluoroscopy times shorter, versus Time to mobilization frame was group, post-operative...
Abstract Renal ischemia‐reperfusion injury (IRI) is one of the most common causes acute kidney (AKI), which closely related to high morbidity and mortality. However, pathogenesis underlying renal IRI complex not fully defined. N6‐methyladenosine (m6A) was recently found be an abundant modification in mammalian messenger RNAs. It implicated various biological processes, while role m6A illustrated. Here we show that m6A‐methylated RNA level its methyltransferase METTL14 are elevated human AKI...
Interfacial water structure on a polymer surface in (or hydration) is related to the antifouling activity of polymer. Zwitterionic materials exhibit excellent due their strong hydration. It was proposed replace zwitterionic polymers using mixed charged because it much easier prepare samples with lower costs. In this study, sum frequency generation (SFG) vibrational spectroscopy, we investigated interfacial structures surfaces and how such change while being exposed salt solutions protein...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDNA Mismatch Binding and Incision at Modified Guanine Bases by Extracts of Mammalian Cells: Implications for Tolerance to DNA Methylation DamageShaun Griffin, Pauline Branch, Yao-Zhong Xu, Peter KarranCite this: Biochemistry 1994, 33, 16, 4787–4793Publication Date (Print):April 1, 1994Publication History Published online1 May 2002Published inissue 1 April...
We studied the substrate specificity of human G:T mismatch-specific thymine glycosylase that initiates repair and G:U base mismatches to G:C pairs. Such arise when 5-methylcytosine or cytosine deaminate spontaneously (and hydrolytically) in DNA. Substrates were 45-bp DNA heteroduplexes bore single G:T, m6G:T, 2,6-diaminopurine:T, 2-amino-6-(methylamino)purine:T, 2-aminopurine:T, G:m4T mispairs. The bases 5' poorly matched G altered selected substrates yield mispairs four different contexts,...
Excited-state dynamics of 4-thiothymidine (S4-TdR) and its photosensitization to molecular oxygen in solution with UVA irradiation were investigated. Absorption emission spectra measurements revealed that photolysis S4-TdR gives rise a population T1(ππ*), following S2(ππ*) → S1(nπ*) internal conversion. In transient absorption measurement, the 355 nm laser gave broad (380−600 nm) bands triplet S4-TdR. The time-resolved thermal lensing (TRTL) signal containing component due decay was clearly...
Ultrafast intersystem crossing of UVA-sensitive 4-thiothymidine in aqueous solution was investigated by pump−probe transient absorption. 4-Thiothymidine the excited singlet state exhibited a distinctively different photophysical pathway from that thymidine, with to triplet manifold being dominant and formation completed within approximately 10 ps. This further supported quantum chemical calculations. We propose unique behavior photoexcited results its distinctive molecular electronic...
XRCC4 and XLF are structurally related proteins important for DNA Ligase IV function. forms a tight complex with while interacts directly XRCC4. Both form homodimers that can polymerize as heterotypic filaments independently of IV. Emerging structural in vitro biochemical data suggest together generate filamentous structure promotes bridging between molecules. Here, we show ablating XRCC4's affinity results repair deficits including surprising deficit VDJ coding, but not signal end joining....
The classic nonhomologous end-joining (c-NHEJ) pathway is largely responsible for repairing double-strand breaks (DSBs) in mammalian cells. XLF stimulates the XRCC4/DNA ligase IV complex by an unknown mechanism. interacts with XRCC4 to form filaments of alternating and dimers that bridge DNA ends vitro, providing a mechanism which might stimulate ligation. Here, we characterize two mutants do not interact cannot or vitro. One mutant fully sufficient stimulating ligation XRCC4/Lig4 vitro;...
Acute kidney injury (AKI) constitutes a prevalent clinical syndrome characterized by elevated morbidity and mortality rates, emerging as significant public health issue. This study investigates the interplay between endoplasmic reticulum (ER) stress, unfolded protein response (UPR), ER-associated degradation (ER-phagy) in pathogenesis of AKI. We employed four distinct murine models AKI-induced contrast media, ischemia-reperfusion injury, cisplatin, folic acid-to elucidate relationship...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of DNA containing modified bases by post-synthetic substitution. Synthesis oligomers 4-substituted thymine: O4-alkylthymine, 5-methylcytosine, N4-dimethylamino-5-methylcytosine, and 4-thiothymineYao Zhong Xu, Qinguo Zheng, Peter F. SwannCite this: J. Org. Chem. 1992, 57, 14, 3839–3845Publication Date (Print):July 1, 1992Publication History Published online1 May 2002Published inissue 1 July...
Abstract Background Two fifths of the world's population is at risk from dengue. The absence effective drugs and vaccines leaves vector control as primary intervention tool. Understanding dengue virus (DENV) host interactions essential for development novel strategies. availability genome sequences both human mosquito greatly facilitates genome-wide studies DENV-host interactions. Results We developed first draft protein interaction network using a computational approach. weighted includes...