A5016939757- Effects and risks of endocrine disrupting chemicals
- Genetically Modified Organisms Research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Estrogen and related hormone effects
- Computational Drug Discovery Methods
- Plant Toxicity and Pharmacological Properties
- Animal testing and alternatives
- Toxic Organic Pollutants Impact
- Marine Biology and Environmental Chemistry
- Pharmacogenetics and Drug Metabolism
- Metabolomics and Mass Spectrometry Studies
- Carcinogens and Genotoxicity Assessment
- Food Allergy and Anaphylaxis Research
- Drug-Induced Hepatotoxicity and Protection
- Viral Infectious Diseases and Gene Expression in Insects
- Immunodeficiency and Autoimmune Disorders
- Nanoparticles: synthesis and applications
- Mycotoxins in Agriculture and Food
- Transgenic Plants and Applications
- Pesticide Exposure and Toxicity
- Birth, Development, and Health
- Immunotherapy and Immune Responses
- Environmental Toxicology and Ecotoxicology
- Per- and polyfluoroalkyl substances research
Wageningen University & Research
2015-2024
Maastricht University
2010-2015
Netherlands Metabolomics Centre
2010-2015
In-Q-Tel
2014
Cornell University
2007-2008
Boston College
2008
TiFN
2003-2006
Leiden University Medical Center
1998-2000
Eurotransplant
2000
Ministry of Agriculture, Nature and Food Quality
2000
We report the results of a 28-day oral exposure study in rats, exposed to <20 nm noncoated, or <15 PVP-coated silver nanoparticles ([Ag] = 90 mg/kg body weight (bw)), AgNO(3) 9 bw), carrier solution only. Dissection was performed at day 29, and after wash-out period 1 8 weeks. Silver present all examined organs with highest levels liver spleen for treatments. concentrations were highly correlated amount Ag(+) nanoparticle suspension, indicating that mainly Ag(+), much lesser extent...
The presence, dissolution, agglomeration state, and release of materials in the nano-size range from food containing engineered nanoparticles during human digestion is a key question for safety assessment these materials. We used an vitro model to mimic digestion. Food products subjected included (i) hot water, (ii) coffee with powdered creamer, (iii) instant soup, (iv) pancake which either contained silica as additive E551, or form synthetic amorphous 32 nm SiO2 particles were added....
Synthetic Amorphous Silica (SAS) is commonly used in food and drugs. Recently, a consumer intake of silica from was estimated at 9.4 mg/kg bw/day, which 1.8 bw/day to be the nano-size range. Food products containing SAS have been shown contain nanometer size range (i.e. 5 – 200 nm) up 43% total content. Concerns raised about possible adverse effects chronic exposure nanostructured silica. Rats were orally exposed 100, 1000 or 2500 SAS, 500 NM-202 (a representative for OECD testing) 28 days,...
Applications of nanoparticles in the food sector are eminent. Silver among most frequently used, making consumer exposure to silver inevitable. Information about uptake through intestines and possible toxic effects is therefore very important but still lacking. In present study, we used an vitro model for human intestinal epithelium consisting Caco-2 M-cells study passage their ionic equivalents assess on whole-genome mRNA expression. This intestine was exposed four sizes 4 h. Exposure ions...
Abstract Per- and polyfluoroalkyl substances (PFASs) are omnipresent in the environment, food chain, humans. Epidemiological studies have shown a positive association between serum levels of perfluorooctanoic acid (PFOA) perfluorooctane sulfonic (PFOS), increased cholesterol and, some cases, also triglyceride levels. However, causality has been questioned, as animal studies, well human trial, showed decrease no effects or plasma triglycerides. To obtain more insight into PFASs on these...
Abstract Background Novel proteins entering the food chain, for example by genetic modification of plants, have to be tested allergenicity. Allermatch™ http://allermatch.org is a webtool efficient and standardized prediction potential allergenicity peptides according current recommendations FAO/WHO Expert Consultation, as outlined in Codex alimentarius. Description A query amino acid sequence compared with all known allergenic retrieved from protein databases using sliding window approach....
In vitro cell systems together with omics methods represent promising alternatives to conventional animal models for toxicity testing. Transcriptomic and proteomic approaches have been widely applied in but relatively few studies used metabolomics. Therefore, the goal of present study was develop an untargeted methodology performing reproducible metabolomics on systems. The human liver line HepG2, well-known hepatotoxic non-genotoxic carcinogen 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD),...
Studies in mice have shown that PPARα is an important regulator of lipid metabolism liver and key transcription factor involved the adaptive response to fasting. However, much less known about role human liver. Here we set out study function via analysis whole genome gene regulation slices treated with agonist Wy14643. Quantitative PCR indicated well expressed classical targets PLIN2, VLDLR, ANGPTL4, CPT1A PDK4 are robustly induced by activation. Transcriptomics 617 genes were upregulated...
Adverse outcome pathways (AOPs) describe causal relationships between molecular perturbation and adverse cellular effects are being increasingly adopted for linking in vitro mechanistic toxicology to vivo data from regulatory toxicity studies. In this work, a case study was performed by developing bioassay toolbox assess key events the recently proposed AOP chemically induced liver steatosis. The is comprised of assays measure nuclear receptor activation, gene protein expression, lipid...
In this study five types of herbal teas were used to quantify the effect comminution leaves on resulting PA exposure. Results show that levels extracted from intact consistently lower than comminuted tea leaves. The Margin Exposure (MOE) approach was applied evaluate consequences difference for associated risks in scenario lifetime Furthermore, we considered medicinal use these shorter-than-lifetime exposure scenarios, and also analysed eight medicines 19 previously plant food supplements....
The mode of action silver nanoparticles (AgNPs) is suggested to be exerted through both Ag+ and AgNP dependent mechanisms. Ingestion one the major NP exposure routes, potential effects are often studied using Caco-2 cells, a well-established model for gut epithelium. MCF-7 cells epithelial breast cancer with extensive well-characterized toxicogenomics profiles. In present study, we aimed gain deeper understanding cellular molecular responses in after order evaluate whether derived from...
Pyrrolizidine alkaloids (PAs) are secondary metabolites from plants that have been found in substantial amounts herbal supplements, infusions and teas. Several PAs cause cancer animal bioassays, mediated via a genotoxic mode of action, but for the majority PAs, carcinogenicity data lacking. It is assumed risk assessment all same potency as riddelliine, which considered to be one most potent carcinogenic rats. This may overestimate risks, since many expected lower potencies. In this study we...
The goal of the present study was to develop an online web-based toolbox that contains generic physiologically based kinetic (PBK) models for rats and humans, including underlying calculation tools predict plasma protein binding tissue:plasma distribution, be used quantitative in-vitro-to-in-vivo extrapolations (QIVIVE). PBK within allow first estimations internal tissue concentrations chemicals made, on logP pKa values intestinal uptake intrinsic hepatic clearance. As a case study, oral...
Abstract Per- and polyfluoroalkyl substances (PFASs) are omnipresent have been shown to induce a wide range of adverse health effects, including hepatotoxicity, developmental toxicity, immunotoxicity. The aim the present work was assess whether human HepaRG liver cells can be used obtain insight into differences in hepatotoxic potencies series PFASs. Therefore, effects 18 PFASs on cellular triglyceride accumulation (AdipoRed assay) gene expression (DNA microarray for PFOS RT-qPCR all PFASs)...