A5060203160- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- COVID-19 Clinical Research Studies
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- SARS-CoV-2 detection and testing
- Virology and Viral Diseases
- Adipokines, Inflammation, and Metabolic Diseases
- Viral gastroenteritis research and epidemiology
- Cell Image Analysis Techniques
- Respiratory viral infections research
- HIV Research and Treatment
- Dermatological and COVID-19 studies
- Malaria Research and Control
- COVID-19 diagnosis using AI
- Virus-based gene therapy research
- Herpesvirus Infections and Treatments
- Reproductive tract infections research
- Poxvirus research and outbreaks
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- Long-Term Effects of COVID-19
- Animal Virus Infections Studies
Inserm
2022-2025
Université Paris-Saclay
2022-2025
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2022-2025
Infectious Disease Models and Innovative Therapies
2022-2025
CEA Paris-Saclay
2022-2025
Chikungunya virus (CHIKV) is a reemerging mosquito-borne alphavirus responsible for numerous outbreaks. can cause debilitating acute and chronic disease. Thus, the development of safe effective CHIKV vaccine an urgent global health priority. This study evaluated effectiveness live-attenuated VLA1553 against WT infection by using passive transfer sera from vaccinated volunteers to nonhuman primates (NHP) subsequently exposed established serological surrogate protection. We demonstrated that...
Abstract The characterization of vaccine distribution to relevant tissues after in vivo administration is critical understanding their mechanisms action. Vaccines based on mRNA lipid nanoparticles (LNPs) are now being widely considered against infectious diseases and cancer. Here, we used imaging approaches compare the trafficking two LNP formulations encapsulating following intramuscular administration: DLin-MC3-DMA (MC3) recently developed DOG-IM4. formulated DOG-IM4 LNPs persisted at...
Abstract The COVID-19 pandemic has caused at least 780 million cases globally. While available treatments and vaccines have reduced the mortality rate, spread evolution of virus are ongoing processes. Despite extensive research, long-term impact SARS-CoV-2 infection is still poorly understood requires further investigation. Routine analysis provides limited access to tissues patients, necessitating alternative approaches investigate viral dissemination in organism. We address this issue by...
Background Chlamydiosis, a sexually transmitted infection (STI) induced by Chlamydia trachomatis (CT), increases local inflammation (cytokine production, recruitment of immune cells such as neutrophils). Few is known on the impact CT phenotype cervicovaginal neutrophils. Vaginal microbiota (VM) key factor in regulation responses and STI acquisition where Lactobacillus spp are associated with protection. In this study, VM cynomolgus macaques was enriched crispatus after metronidazole...
Abstract The SARS-CoV-2 pandemic revealed the rapid evolution of circulating strains. This led to new variants carrying mostly mutations within receptor binding domain, which is immunodominant upon immunization and infection. In order steer immune response away from RBD epitopes more conserved domains, we generated S glycoprotein trimers without stabilized them by formaldehyde cross-linking. cryoEM structure demonstrated that SΔRBD folds into native prefusion conformation, one specific...
The well documented association between obesity and the severity of SARS-CoV-2 infection raises question whether adipose tissue (AT) is impacted during this infection. Using a model in cynomolgus macaques, we detected virus within subcutaneous AT (SCAT) but not visceral (VAT) or epicardial on day 7 post-infection. We sought to determine mechanisms responsible for selective detection observed higher levels angiotensin-converting-enzyme-2 mRNA expression SCAT than VAT. Lastly, evaluated...
SARS-CoV-2 infection has been associated with intestinal mucosal barrier damage, leading to microbial and endotoxin translocation, heightened inflammatory responses, aggravated disease outcomes. This study aimed investigate the immunological mechanisms impaired function. We conducted a comprehensive analysis of gut damage inflammation markers phenotypic characterization myeloid lymphoid populations in ileum colon SARS-CoV-2-exposed macaques during both acute resolved phases. Our findings...
Abstract Passive immunization using broadly neutralizing antibodies (bNAbs) is investigated in clinical settings to inhibit HIV-1 acquisition due the lack of a preventive vaccine. However, bNAbs efficacy against highly infectious cell-associated virus transmission has been overlooked. mediated by infected cells present body fluids likely dominates infection and aids evading antibody-based immunity. Here, we show that anti-N-glycans/V3 loop bNAb 10-1074 formulated for topical vaginal...
<title>Abstract</title> The COVID-19 pandemic has caused nearly 780 million cases globally. While available treatments and vaccines have allowed a reduction of the mortality rate, spread virus is still evolving quickly, resulting in emergence new variants. Despite extensive research, long-term impact SARS-CoV-2 infection poorly understood requires further investigation. Routine analysis provides limited access to tissues patients, necessitating alternative approaches investigate viral...
PARVAX is a genetic vaccine platform based on an adeno-associated vector that has demonstrated to elicit potent, durable, and protective immunity in nonhuman primates (NHPs) after single dose. Here, we assessed immunogenicity following prime-boost regimen against SARS-CoV-2. In mice, low-dose prime followed by higher-dose boost elicited potent neutralizing antibody responses distinct cross-reactivity profiles, depending the antigen used booster vaccine. However, anti-vector developed mice...
<title>Abstract</title> The monkeypox virus (MPXV) outbreak of 2022 caused a human disease with unusual epidemiological and clinical features, notably an increase in human-to-human transmission through sexual contact, predominantly among men who have sex (MSM). This evolution underscores the need to reassess prevention control strategies context sexually transmitted disease. Here, we show that rectal challenge cynomolgus macaques clade IIb MPXV isolate mimics transmission, leading infection,...