A5032955596- Carcinogens and Genotoxicity Assessment
- Nuclear Structure and Function
- CRISPR and Genetic Engineering
- Microtubule and mitosis dynamics
- Molecular Biology Techniques and Applications
- DNA Repair Mechanisms
University of Connecticut
2023
Litron Laboratories (United States)
2020
MultiFlow® DNA Damage-p53, γH2AX, Phospho-Histone H3 is a miniaturized, flow cytometry-based assay that provides genotoxic mode of action information by distinguishing clastogens, aneugens, and nongenotoxicants. Work to date has focused on the p53-competent human cell line TK6. While mammalian genotoxicity assays typically supply exogenous metabolic activation in form concentrated rat liver S9, this less-than-ideal approach for several reasons, including 3Rs considerations. Here, we describe...
ABSTRACT 53BP1 (also known as TP53BP1) is a key mediator of the non-homologous end joining (NHEJ) DNA repair pathway, which primary pathway in interphase cells. However, mitotic functions are less well understood. Here, we describe stress bodies (MSBs) formed cancer cell lines response to delayed mitosis. These displayed liquid–liquid phase separation characteristics, were close centromeres, and included lamin A/C protein RIF1. After release from arrest, MSBs decreased number moved away...