A5091287602- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Fungal and yeast genetics research
- RNA modifications and cancer
- Plant Disease Resistance and Genetics
- Cancer-related gene regulation
- Nuclear Structure and Function
- Heat shock proteins research
- Endoplasmic Reticulum Stress and Disease
- CRISPR and Genetic Engineering
- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- Plant Gene Expression Analysis
- Plant-Microbe Interactions and Immunity
Université Paris Cité
2016-2025
Centre National de la Recherche Scientifique
2016-2025
Université Paris-Saclay
2018-2025
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2022-2025
CEA Paris-Saclay
2022-2025
Institut de Biologie Intégrative de la Cellule
2022-2025
Expression Génétique Microbienne
2025
Institut de Biologie Physico-Chimique
2025
Institut Jacques Monod
2016-2022
Sorbonne Paris Cité
2016-2020
The Set1 family of histone H3 lysine 4 (H3K4) methyltransferases is highly conserved from yeast to human. Here we show that the complex (Set1C) directly binds RNA in vitro through regions comprise double recognition motifs (dRRM) and N-SET domain within its subunit Spp1. To investigate functional relevance binding, performed UV crosslinking (CRAC) for polymerase II parallel with ChIP-seq experiments. nascent transcripts dRRM. binding important define appropriate topology Set1C distribution...
mRNA homoeostasis is favoured by crosstalk between transcription and degradation machineries. Both the Ccr4-Not Xrn1-decaysome complexes have been described to influence transcription. While has shown directly stimulate elongation, information available on how Xrn1 influences scarce contradictory. In this study we addressed issue mapping RNA polymerase II (RNA pol II) at high resolution, using CRAC BioGRO-seq techniques in Saccharomyces cerevisiae. We found significant effects of...
Abstract Small nucleolar RNAs are non-coding transcripts that guide chemical modifications of RNA substrates and modulate gene expression at the epigenetic post-transcriptional levels. However, extent their regulatory potential underlying molecular mechanisms remain poorly understood. Here, we identify a conserved, previously unannotated intronic C/D-box snoRNA, termed snR107 , hosted in fission yeast long mamRNA carrying two independent cellular functions. On one hand, guides site-specific...
Abstract Cellular homeostasis is maintained by surveillance mechanisms that intervene at virtually every step of gene expression. In the nucleus, yeast chromatin remodeler Isw1 holds back maturing mRNA ribonucleoparticles to prevent their untimely export, but whether this activity operates beyond quality control biogenesis regulate expression unknown. Here, we identify encoding central effector unfolded protein response (UPR) HAC1 , as an RNA target. The direct binding 3’ untranslated region...
Small nucleolar RNAs are non-coding transcripts that guide chemical modifications of RNA substrates and modulate gene expression at the epigenetic post-transcriptional levels. However, extent their regulatory potential underlying molecular mechanisms remain poorly understood. Here, we identify a conserved, previously unannotated intronic C/D-box snoRNA, termed snR107, hosted in fission yeast long mamRNA carrying two independent cellular functions. On one hand, snR107 guides site-specific 25S...
Heterochromatic gene silencing relies on combinatorial control by specific histone modifications, the occurrence of transcription, and/or RNA degradation. Once nucleated, heterochromatin propagates within defined chromosomal regions and is maintained throughout cell divisions to warrant proper genome expression integrity. In fission yeast Schizosaccharomyces pombe, Ccr4-Not complex partakes in silencing, but its relative contribution distinct domains role nucleation versus spreading have...
Abstract Precise nucleosome organization at eukaryotic promoters is thought to be generated by multiple chromatin remodeler (CR) enzymes and affect transcription initiation. Using an integrated analysis of binding displacement activity following rapid depletion, we investigate the interplay between these their impact on in budding yeast. We show that many are acted upon CRs operate either cooperatively or opposition position key start site-associated +1 nucleosome. Functional assays suggest...
Abstract Heterochromatic gene silencing relies on combinatorial control by specific histone modifications, the occurrence of transcription, and/or RNA degradation. Once nucleated, heterochromatin propagates within defined chromosomal regions and is maintained throughout cell divisions to warrant proper genome expression integrity. The fission yeast Ccr4-Not complex has been involved in silencing, but its relative contribution distinct domains role nucleation versus spreading have remained...
ABSTRACT The fidelity of transcription initiation is essential for accurate gene expression, but the determinants start site selection are not fully understood. Rap1 and other General Regulatory Factors (GRFs) control expression many genes in yeast. We show that depletion these factors induces widespread ectopic within promoters. This generates novel non-coding RNAs transcript isoforms with diverse stability, profoundly altering coding potential transcriptome. Ectopic strongly correlates...
ABSTRACT Cellular homeostasis requires the coordination of several machineries concurrently engaged on DNA. Wide-spread transcription can interfere with other processes and transcription-replication conflicts (TRCs) threaten genome stability. The conserved Sen1 helicase terminates non-coding transcription, but also interacts replisome reportedly resolves genotoxic R-loops. prevents genomic instability how this relates to its molecular functions remains unclear. We generated high-resolution,...
Abstract mRNA homeostasis is favored by crosstalk between transcription and degradation machineries. Both the Ccr4-Not Xrn1-decaysome complexes have been described to influence transcription. While has shown directly stimulate elongation, information available on how Xrn1 influences scarce contradictory. In this study we addressed issue mapping RNA polymerase II (RNA pol II) at high resolution, using CRAC BioGRO-seq techniques in Saccharomyces cerevisiae . We found significant effects of...