A5055027472- Pain Mechanisms and Treatments
- Nerve injury and regeneration
- Glycosylation and Glycoproteins Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Cellular transport and secretion
- Carbohydrate Chemistry and Synthesis
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Infant Nutrition and Health
- Alzheimer's disease research and treatments
- Botulinum Toxin and Related Neurological Disorders
- Extracellular vesicles in disease
- interferon and immune responses
- Cytokine Signaling Pathways and Interactions
- Single-cell and spatial transcriptomics
- Amyotrophic Lateral Sclerosis Research
- RNA regulation and disease
- Hereditary Neurological Disorders
- Proteoglycans and glycosaminoglycans research
- Tissue Engineering and Regenerative Medicine
- Photoreceptor and optogenetics research
- Memory and Neural Mechanisms
- biodegradable polymer synthesis and properties
- NF-κB Signaling Pathways
- Viral gastroenteritis research and epidemiology
Aarhus University
2012-2022
Lundbeck Foundation
2013-2019
Danish Diabetes Academy
2019
Abstract Satellite glial cells (SGCs) are homeostatic enveloping the somata of peripheral sensory and autonomic neurons. A wide variety neuronal stressors trigger activation SGCs, contributing to, for example, neuropathic pain through modulation activity. However, compared to neurons other nervous system, SGCs have received modest scientific attention very little is known about SGC biology, possibly due experimental challenges associated with studying them in vivo vitro. Utilizing a recently...
The present work reports isolation and characterization of a highly glycosylated protein from bovine milk fat globule membranes, known as PAS III. Partial amino‐acid sequencing the purified allowed construction degenerate oligonucleotide primers, enabling full‐length cDNA encoding 330 residues. N‐terminal derived peptides confirmed 76% sequence demonstrated presence cleavable signal peptide 23 residues, leaving mature 307 amino acids. Database searches showed no homology to any other...
The development and progression of Alzheimer's disease is linked to excessive production toxic amyloid-β peptide, initiated by β-secretase cleavage the amyloid precursor protein (APP). In contrast, soluble APPα (sAPPα) generated α-secretase known stimulate dendritic branching enhance synaptic function. Regulation APP processing, shift from neurotrophic neurotoxic metabolism remains poorly understood, but cellular localization its interaction with various receptors considered important. We...
SORCS3 is an orphan receptor of the VPS10P domain family, a group sorting and signaling receptors central to many pathways in control neuronal viability function. highly expressed CA1 region hippocampus, but relevance this for hippocampal activity remained absolutely unclear. Here, we show that localizes postsynaptic density loss gene-targeted mice abrogates NMDA receptor-dependent -independent forms long-term depression (LTD). Consistent with synaptic retraction, SORCS3-deficient suffer...
Blocking of sortilin ligand binding prevents neuropathic pain by inhibiting BDNF-induced spinal KCC2 down-regulation.
Abstract Sortilin is a multifunctional receptor involved in sorting and apoptosis. We have previously reported 2.0‐Å structure of the Vps10 ectodomain complex with one its ligands, tridecapeptide neurotensin. Here we set out to further characterize structural properties sortilin interaction To this end, determined new 2.7 Å using crystal grown 10‐fold increased concentration second peptide fragment was observed within β‐propeller, which may principle either represent molecule neurotensin or...
Satellite glial cells (SGCs) surrounding the neuronal somas in peripheral sensory ganglia are sensitive to stressors, which induce their reactive state. It is believed that such induced gliosis affects signaling properties of primary neurons and an important component neuropathic phenotype leading pain other disturbances. Efforts understand manipulate relies on reliable markers confirm SGC reactivity ultimately efficacy targeted intervention. Glial fibrillary acidic protein (GFAP) currently...
Background: Satellite glial cells (SGCs) tightly surround and support primary sensory neurons in the peripheral nervous system are increasingly recognized for their involvement development of neuropathic pain following nerve injury. SGCs difficult to investigate due flattened shape tight physical connection vivo rapid changes phenotype protein expression when cultured vitro. Consequently, several aspects SGC function under normal conditions as well after a injury remain be explored. The...
Sortilin is a type I membrane glycoprotein belonging to the vacuolar protein sorting 10 (Vps10p) family of receptors and most abundantly expressed in central nervous system. has emerged as key player regulation neuronal viability been implicated possible therapeutic target range disorders. Here, identification AF40431, first reported small-molecule ligand sortilin, reported. Crystals sortilin-AF40431 complex were obtained by co-crystallization structure was solved 2.7 Å resolution. AF40431...
Targeted protein degradation (TPD) using PROteolysis TArgeting Chimeras (PROTACs) is a rapidly emerging therapeutic strategy for difficult-to-drug cytosolic proteins. PROTACs are heterobifunctional small molecules that bridge the target with an E3 ubiquitin ligase, destining it by proteasome. They have potential to be orally available and act catalytically, switching pharmacology from occupancy-driven event-driven (1-3). Here we present targeted of extracellular proteins reshaping...
Satellite glial cells (SGCs) envelop the somata, axon hillock, and initial segment of sensory neurons in dorsal root ganglia (DRG), playing a critical role regulating neuronal microenvironment. While DRG have been extensively studied classified based on size, molecular markers, functional characteristics, very little is still known about SGC heterogeneity its potential implications processing DRG. Single cell transcriptional analyses proposed existence subtypes, yet situ validation, spatial...
Cardiotrophin-like cytokine:cytokine-like factor-1 (CLC:CLF-1) is a heterodimeric neurotropic cytokine that plays crucial role during neuronal development. Mice lacking CLC:CLF-1 die soon after birth due to suckling defect and show reduced numbers of motor neurons. Humans carrying mutations in develop similar disorders, known as Sohar-Crisponi or cold-induced sweating syndrome, have high risk early death. It well CLC binds the ciliary neurotrophic factor receptor α (CNTFRα) prerequisite for...
The multifunctional type 1 receptor sortilin is involved in endocytosis and intracellular transport of ligands. short domain binds several cytoplasmic adaptor proteins (e.g., the AP-1 complex GGA1 to -3), most which target two well-defined motifs: a C-terminal acidic cluster dileucine motif YXXΦ proximal third domain. Both motifs contribute as well Golgi-endosome trafficking sortilin. harbors serine residue, subject phosphorylation by casein kinase. Phosphorylation this residue known...
While electrical circuits are a fundamental part of neural activity, the soft microstructural extracellular environment plays vital role in driving morphogenesis neurons. To leverage this, we combine wireless electromagnetic stimulation system with microfilamented anisotropic GelMA hydrogels to promote and guide morphogensis. We show that support growth dorsal root ganglion as potential peripheral conduit formation hippocampal neurospheres networking. The central nervous has limited...
Abstract Satellite glial cells (SGCs) tightly surround and support primary sensory neurons in the peripheral nervous system are increasingly recognized for their involvement development of neuropathic pain following nerve injury. The SGCs difficult to investigate due flattened shape tight physical connection vivo rapid changes phenotype protein expression when cultured vitro . Consequently, several aspects SGC function under normal conditions as well after a injury remain be explored. recent...