A5020905419- Immune Cell Function and Interaction
- Mast cells and histamine
- T-cell and B-cell Immunology
- MicroRNA in disease regulation
- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- IL-33, ST2, and ILC Pathways
- Cancer Immunotherapy and Biomarkers
- Asthma and respiratory diseases
- RNA Research and Splicing
- Immune cells in cancer
- interferon and immune responses
- Cytokine Signaling Pathways and Interactions
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Immune responses and vaccinations
- Immune Response and Inflammation
- Circular RNAs in diseases
- Reproductive System and Pregnancy
- Extracellular vesicles in disease
- Signaling Pathways in Disease
- Polyamine Metabolism and Applications
Università della Svizzera italiana
2015-2024
Harvard University
2002-2006
University of Milan
2004-2005
Boston University
2004
Dana-Farber/Boston Children's Cancer and Blood Disorders Center
2002-2004
University of Arizona
2001-2002
Vita-Salute San Raffaele University
1997-1998
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
1997-1998
Istituti di Ricovero e Cura a Carattere Scientifico
1997-1998
MicroRNAs (miRNAs) are a class of recently discovered noncoding RNA genes that post-transcriptionally regulate gene expression. It is becoming clear miRNAs play an important role in the regulation expression during development. However, mammals, data principally based on whole tissue analysis and still very incomplete.We used oligonucleotide arrays to analyze miRNA murine hematopoietic system. Complementary oligonucleotides capable hybridizing 181 were immobilized membrane probed with...
MicroRNAs (miRNAs) are regulatory molecules able to influence all aspects of the biology a cell. They have been associated with diseases such as cancer, viral infections, and autoimmune diseases, in recent years, they also emerged important regulators immune responses. MiR-146a particular is rapidly gaining importance modulator differentiation function cells innate well adaptive immunity. Given its regulating key cellular functions, it not surprising that miR-146a expression was found...
Significance Methylation of genomic DNA is an epigenetic modification at the interface between genetic information and environmental stimuli underlying many phenotypic variations in human population as well pathogenesis complex diseases. Accordingly, mutations de novo methyltransferase enzyme DNMT3A have been identified a number diseases, including mast cell-related disorders. However, role methylation regulating cell physiology still needs to be elucidated. Here, we found that Dnmt3a plays...
While pancreatic ductal adenocarcinomas (PDACs) are addicted to KRAS-activating mutations, inhibitors of downstream KRAS effectors, such as the MEK1/2 kinase inhibitor trametinib, devoid therapeutic effects. However, extensive rewiring regulatory circuits driven by attenuation pathway may induce vulnerabilities relevance. An in-depth molecular analysis transcriptional and epigenomic alterations occurring in PDAC cells initial hours after inhibition trametinib unveiled induction endogenous...
Abstract MicroRNAs (miRNAs) constitute a large family of small noncoding RNAs that have emerged as key posttranscriptional regulators in wide variety organisms. Because any one miRNA can potentially regulate expression distinct set genes, differential shape the repertoire proteins are actually expressed during development and differentiation or disease. Here, we used mast cells model to investigate role miRNAs differentiated innate immune found miR-221–222 significantly up-regulated upon...
Mast cells have essential effector and immunoregulatory functions in IgE-associated allergic disorders certain innate adaptive immune responses, but the role of miRNAs regulating mast cell is almost completely unexplored. To examine activation-induced miRNA miR-221 mouse cells, we developed robust lentiviral systems for overexpression depletion. While favored adhesion migration towards SCF or antigen trans-well assays, as well cytokine production degranulation response to IgE-antigen...
The nuclear factor of activated T cells (NFAT) plays a key role in gene transcription both immune and non-immune cell types. Genetic ablation individual NFAT proteins produces complex phenotypes mice; particular, NFAT1–/– NFAT2–/– show overproduction underproduction IL-4, respectively. We have taken positive approach to the question whether these two family members differentially regulate IL-4 transcription. Using constitutively-active with alanine substitutions instead phosphorylated serine...
The immunoregulatory cytokine interleukin 10 (IL-10) modulates the function of diverse immune and non-immune cells. Here, we examine chromatin structural changes associated with IL10 gene transcription by naive differentiated murine T Naive cells lack DNase I hypersensitive (HS) sites in vicinity gene, whereas display a strong 3' constitutive HS site as well several inducible sites. majority map to regions that are strongly conserved sequence between mouse human genomes. In committed Th1...
The transcription factor NF-κB regulates the expression of a broad number genes central to immune and inflammatory responses. We identified new molecular network that comprises specifically family member NF-κB1 (p50) miR-146a, we show in mast cells it contributes regulation cell homeostasis survival, while T lymphocytes modulates memory formation. Increased survival was due unbalanced pro- antiapoptotic factors particularly complete inability p50-deleted induce which context acted as...
Abstract Mast cells are central players in allergy and asthma, their dysregulated responses lead to reduced quality of life life-threatening conditions such as anaphylaxis. The RNA modification N 6 -methyladenosine (m A) has a prominent impact on immune cell functions, but its role mast remains unexplored. Here, by optimizing tools genetically manipulate primary cells, we reveal that the m A mRNA methyltransferase complex modulates proliferation survival. Depletion catalytic component Mettl3...
T helper (TH) cell polarization during priming is modulated by a number of signals, but whether to given phenotype also influences recall responses memory TH cells relatively unknown. Here we show that miR-181a selectively induced in both human and mouse naive differentiating into the TH17, not TH1 or TH2 subset. In TH17 cells, regulates cognate antigens through modulation ERK phosphorylation. By enhancing signalling cascade from T-cell receptor, such molecular network reduces threshold...