A5065974461- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Redox biology and oxidative stress
- Heat shock proteins research
- Glutathione Transferases and Polymorphisms
- Synthesis and Biological Evaluation
- Genetics, Bioinformatics, and Biomedical Research
- Enzyme function and inhibition
- Digital Imaging for Blood Diseases
- Milk Quality and Mastitis in Dairy Cows
- Effects of Environmental Stressors on Livestock
- Aerospace and Aviation Technology
- Genetic and phenotypic traits in livestock
- Rabbits: Nutrition, Reproduction, Health
- Cell Image Analysis Techniques
- Phytochemical compounds biological activities
- biodegradable polymer synthesis and properties
- Enzyme Structure and Function
- Bone Tissue Engineering Materials
- Subtitles and Audiovisual Media
- Hearing Impairment and Communication
- Reproductive Physiology in Livestock
- Parasites and Host Interactions
- Image Processing Techniques and Applications
- Biotechnology and Related Fields
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2015-2024
Universidade Federal Fluminense
2015-2023
Instituto Vital Brazil (Brazil)
2023
Universidade do Porto
2011-2021
Instituto de Biologia Molecular e Celular
2002-2021
Universidade de Mogi das Cruzes
2019
Instituto Federal de Educação, Ciência e Tecnologia de Brasília
2012
National Council for Scientific Research
2012
European Space Research and Technology Centre
2006
National University of the Littoral
2001
Significance Peroxiredoxins (Prxs) are highly abundant proteins, which serve two seemingly mutually exclusive roles as peroxidases and molecular chaperones. Little is known about the precise mechanism of Prxs’ activation chaperone physiological significance this second function. Here we demonstrate that in Leishmania infantum , reduced Prx provides a crucial, stress-specific reservoir, activated rapidly upon exposure to unfolding stress conditions. Once activated, protects wide range...
Two-cysteine peroxiredoxins are ubiquitous peroxidases that play various functions in cells. In Leishmania and related trypanosomatids, which lack catalase selenium-glutathione peroxidases, the discovery of this family enzymes provided molecular basis for peroxide removal these organisms. report functional relevance one such enzymes, mitochondrial 2-Cys peroxiredoxin (mTXNPx), was investigated along infantum life cycle. mTXNPx null mutants (mtxnpx−) produced by a gene replacement strategy,...
Abstract Many 2-Cys-peroxiredoxins (2-Cys-Prxs) are dual-function proteins, either acting as peroxidases under non-stress conditions or chaperones during stress. The mechanism by which 2-Cys-Prxs switch functions remains to be defined. Our work focuses on Leishmania infantum mitochondrial 2-Cys-Prx, whose reduced, decameric subpopulation adopts chaperone function heat shock, an activity that facilitates the transition from insects warm-blooded host environments. Here, we have solved cryo-EM...
Leishmania parasites cause a set of neglected tropical diseases with considerable public health impact, the leishmaniases, which are often fatal if left untreated. Since current treatments for leishmaniases exhibit high toxicity, low efficacy and prohibitive prices, many laboratories throughout world engaged in research discovery novel chemotherapeutics. This entails necessity screening large numbers compounds against clinically relevant form parasite, obligatory intracellular amastigote,...
Tryparedoxins (TXNs) are oxidoreductases unique to trypanosomatids (including Leishmania and Trypanosoma parasites) that transfer reducing equivalents from trypanothione, the major thiol in these organisms, sulfur-dependent peroxidases other dithiol proteins. The existence of a TXN within mitochondrion trypanosomatids, capable driving crucial redox pathways, is considered requisite for normal parasite metabolism. Here this concept shown not apply Leishmania. First, removal infantum...
The biological activities of a series mesoionic 1,3,4-thiadiazolium-2-aminide derivatives have been studied. most active compounds (MI-HH; MI-3-OCH3; MI-4-OCH3 and MI-4-NO2) were evaluated to determine their effect on trypanothione reductase (TryR) activity in Leishmania sp. Trypanosoma cruzi. Among the assayed compounds, only MI-4-NO2 showed enzyme inhibition extracts from different cultures parasites, which was confirmed using recombinant T. cruzi (TcTryR) infantum (LiTryR). kinetics...
Typical two-cysteine peroxiredoxins (2-Cys-PRXs) are H2O2-metabolizing enzymes whose activity relies on two cysteine residues. Protists of the family Trypanosomatidae invariably express one cytosolic 2-Cys-PRX (cPRX1). However, Leishmaniinae sub-family features an additional isoform (cPRX2), almost identical to cPRX1, except for lack elongated C-terminus with a Tyr-Phe (YF) motif. Previously, PRXs were considered vital components trypanosomatid antioxidant machinery. Here, we shed new light...
Peroxiredoxins are highly conserved thiol peroxidases essential for peroxide detoxification, redox signaling, and chaperone activity. Prx1/AhpC-type peroxiredoxins found throughout the eukaryotic kingdom, where multiple isoforms frequently coexist within same cell even in subcellular compartment. Long thought to form exclusively homooligomeric structures, we reveal that heterooligomerization is a important feature of peroxiredoxins. We demonstrate heterooligomer formation modulates...
2,6,6-Trimethylcyclohex-2-ene-1,4-dione (TMCHD), a norisoprenoid with sweet honey aroma descriptor, is reported for the first time as minor constituent of single-varietal table and fortified wines from demarcated Douro region. Olfactory gas chromatography (GC-O) volatile wine extract, previously isolated by preparative chromatography, indicated presence zone containing an intense descriptor. The targeted odor compound was identified GC-MS, GC-O, Kovats index. Quantitative analysis using...
Significance Leishmaniasis is one of the most dangerous, neglected tropical diseases. Oxidative phosphorylation a known target antipathogenic therapeutics. Herein, we addressed contribution type II NADH dehydrogenase (NDH2) and complex I activities to Leishmania physiology. Our results provide evidence that NDH2 essential throughout parasites’ life cycle, independently presence functional I. Furthermore, sustained expression renders dispensable. This indicates has unique function(s) in...
Forty-eight grazing dairy cows in mid-lactation were randomly assigned to each of eight treatments combining the following factors: protection system (shade vs. no shade), supplementation (0 3.5 kg concentrate/cow/day) and cow parity (multiparous primiparous). The study was conducted during summer at Rafaela Experimental Station, Santa Fe, Argentina. Animal comfort, milk production composition, Wisconsin mastitis test (WMT) somatic cell count (SCC), activity, some blood biochemical...
DNA extraction and polymerase chain reaction are molecular biology techniques widely used in research, diagnostics biotechnology industry. In this work, we report a practical course designed for undergraduate students of health, biological sciences areas. The is five days long emphasizes basic procedures activities. were challenged to use three different protocols isolation (one using commercial silica column two, in-house) no lab animals but themselves. They evaluated the yield, potential...