A5020489213- Genetics, Bioinformatics, and Biomedical Research
- Reproductive System and Pregnancy
- HIV Research and Treatment
- Retinal Diseases and Treatments
- Retinoids in leukemia and cellular processes
- S100 Proteins and Annexins
- Retinal Development and Disorders
- Estrogen and related hormone effects
- Immunotherapy and Immune Responses
- Cancer Genomics and Diagnostics
- Reproductive Physiology in Livestock
- Cytokine Signaling Pathways and Interactions
- Gene expression and cancer classification
- Biochemical and Molecular Research
- Glutathione Transferases and Polymorphisms
- Retinal Imaging and Analysis
- Genomics and Phylogenetic Studies
- Bioinformatics and Genomic Networks
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Selenium in Biological Systems
- Redox biology and oxidative stress
- HIV/AIDS drug development and treatment
- RNA and protein synthesis mechanisms
- Biomedical and Engineering Education
Center for Information Technology
2015-2024
National Institutes of Health
2015-2024
SRA International (United States)
2015-2018
Selenoproteins mediate much of the cancer-preventive properties essential nutrient selenium, but some these proteins have been shown to also cancer-promoting effects. We examined contributions 15kDa selenoprotein (Sep15) and thioredoxin reductase 1 (TR1) cancer development. Targeted down-regulation either gene inhibited anchorage-dependent anchorage-independent growth formation experimental metastases mouse colon carcinoma CT26 cells. Surprisingly, combined deficiency Sep15 TR1 reversed...
ARPE-19 cells are derived from adult human retinal pigment epithelium (RPE). The response of these to the stress serum deprivation mimics some important processes relevant age-related macular degeneration (AMD). Here we extend characterization this using RNASeq and EGSEA gene set analysis over nine days deprivation. This experiment confirmed up-regulation cholesterol lipid-associated pathways that increase levels in cells. expression also identified other AMD progression. There were...
In middle-aged women, the decline of ovarian follicle reserve below a critical threshold marks menopause, leading to hormonal, inflammatory, and metabolic changes linked disease. The highest incidence mortality sporadic cancer (OC) occur at post-menopause, while OC risk is reduced by full-term pregnancies during former fertile life. Herein, we investigate how parity history modulates transcriptome related such declining pool systemic inflammation in reproductively-aged mice. Female C57BL/6...
The third-generation sequencing technology, PacBio, has shown an ability to sequence the HIV virus amplicons in their full length. long read of PaBio offers a distinct advantage comprehensively understand evolution complexity at quasispecies level (i.e. maintaining linkage information variants) comparing short reads from Illumina shotgun sequencing. However, due highnoise nature PacBio reads, it is still challenge build accurate contigs high sensitivity. Most previously developed NGS...
Abstract We assembled teams of genomics professionals to assess whether we could rapidly develop pipelines answer biological questions commonly asked by biologists and others new bioinformatics facilitating analysis high-throughput sequencing data. In January 2015, were on the National Institutes Health (NIH) campus address in DNA-seq, epigenomics, metagenomics RNA-seq subfields genomics. The only two rules for this hackathon that either data used housed at Center Biotechnology Information...
Abstract ARPE-19 cells are derived from adult human retinal pigment epithelium (RPE). The response of these to the stress serum deprivation mimics some important processes relevant age-related macular degeneration (AMD). Here we extend characterization this using RNASeq and EGSEA gene set analysis over nine days deprivation. This experiment confirmed up-regulation cholesterol lipid-associated pathways that increase levels in cells. expression also identified other AMD progression. There were...
The third-generation sequencing technology, PacBio, has shown an ability to sequence the HIV virus amplicons in their full length.The long read of PaBio offers a distinct advantage comprehensively understand evolution complexity at quasispecies level (i.e.maintaining linkage information variants) comparing short reads from Illumina shotgun sequencing.However, due highnoise nature PacBio reads, it is still challenge build accurate contigs high sensitivity.Most previously developed NGS...