More than 18 types of human papillomavirus (HPV) are associated with cervical cancer, the relative importance HPV may vary in different populations.To investigate HPV, age distribution, and risk factors for infection women from Santiago, Chile.We interviewed obtained two specimens a population-based random sample 1,038 sexually active (age range, 15-69 years). Specimens were tested presence DNA using GP5+/6+ primer-mediated PCR cytologic abnormalities by Papanicolaou smears.122 positive DNA,...

Background: Respiratory syncytial virus (RSV) is a major cause of acute lower respiratory infection in infants. The immune response plays leading role the severity disease. We hypothesized that severe RSV disease associated with an impaired characterized by low circulating T lymphocytes and plasma cytokine concentrations. Methods: evaluate vivo responses previously healthy infants their first proven RSV-acute required hospitalization. According to clinical severity, defined using strict...

Human papillomavirus (HPV) and Epstein Barr virus (EBV) have been found in breast carcinomas (BCs) around the world. In this study, fifty-five BCs from Chile were analyzed for HPV EBV presence. addition, HPV-16 viral load/physical status E6/E7 expressions determined.The amplification of a housekeeping gene showed that 46/55 samples (84%) had amplifiable DNA. was detected 4/46 (8.7%) 3/46 (6.5%) BCs. The analysis physical integrated all tumors with relatively low load (range: 0.14 to 33.8...

Storey et al. (1998) implicated the proline/argine polymorphism of codon 72 tumor-suppressor gene p53 in development cervical cancer (CC) with observation that protein is more efficiently inactivated by E6 oncoprotein human papillomavirus arginine as compared its proline isoform.These authors further noted United Kingdom, individuals homozygous for allele were several times susceptible to HPV-associated tumorigenesis proline/arginine heterozygotes.Subsequent studies different countries...

Conclusions. Human papillomavirus (HPV) DNA was detected in 32% of laryngeal carcinoma biopsy samples studied. The genotypes identified were high-risk types, the most frequent being HPV 16. Viral integrated into host genome (genotype 16), providing supporting evidence for a role carcinogenic pathway squamous cell carcinoma. Objective. has been lesions, both benign and neoplastic, with variable frequency (8–60%). These viral agents have proposed as an adjuvant or cofactor head neck...

Based in epidemiological evidence, repetitive ovulation has been proposed to play a role the origin of ovarian cancer by inducing an aberrant wound rupture-repair process surface epithelium (OSE). Accordingly, long term cultures isolated OSE cells undergo vitro spontaneous transformation thus developing tumorigenic capacity upon extensive subcultivation. In this work, C57BL/6 mouse (MOSE) were cultured up passage 28 and their RNA DNA copy number profiles obtained at passages 2, 5, 7, 10, 14,...

Respiratory syncytial virus (RSV) is the major cause of acute lower respiratory tract infection in infants. Winter outbreaks Chile result 5% infected children hospitalized, with 0.01% mortality. Increased evidence indicates that viral and host factors modulate severity infection. Using DNA microarrays, we characterized genome-wide transcriptional response lung mucoepidermoid cells (NCI-H292) at 0, 24, 48, 72 96 hours post-infection (hpi) a single dose RSV/A. During whole studied period,...

In our population, only half of fully nursing women remain amenorrheic 6 months postpartum. The other recover their menstrual cycles between 90-180 days postpartum in spite a high suckling frequency and elevated immunoreactive PRL (IR-PRL) concentrations. To further investigate the association with recovery ovarian function, we compared bioactivity (BIO-PRL) 3-4 who subsequently experienced long (> 180 days; n = 5) or short (< lactational amenorrhea. present study, BIO-PRL plasma was...

Community-acquired pneumonia (CAP) is a worldwide leading cause of death. Recognized risk factors in some severe cases have not been identified. Lymphocytopenia has frequently described CAP. Since IL-7, membrane-bound receptor (IL7Rα;CD127) and soluble IL7Rα (sIL7R) are critical lymphocytes homeostasis, this work we aimed to evaluate the involvement IL-7/IL7Rα axis severity adult CAP, since it explored. The SNPs rs6897932, rs987106, rs3194051 IL7α were genotyped, systemic expression IL7R...

The inhibitory actions of prolactin on gonadal steroidogenesis have been reported in different species and under a variety experimental approaches. In this study, the mechanisms in-vitro effects human (hPRL) follicle stimulating hormone (hFSH)-induced aromatase activity were determined using cultured granulosa cells from diethylstilboestrol (DES)-primed immature rats. Human PRL caused dose-dependent decrease hFSH-induced 17 beta-oestradiol production, even when presence cAMP analogue...

BRCA1 gene mutations account for nearly all families with multiple cases of both early onset breast and/or ovarian cancer and about 30% hereditary cancer.Although to date more than 1,237 distinct mutations, polymorphisms, variants have been described, several found be recurrent in this gene.We analyzed 63 Chilean breast/ovarian eighteen frequent mutations.The analysis the five exons two introns which these are located was made using mismatch PCR assay, ASO hybridization restriction fragment...

In middle-aged women, the decline of ovarian follicle reserve below a critical threshold marks menopause, leading to hormonal, inflammatory, and metabolic changes linked disease. The highest incidence mortality sporadic cancer (OC) occur at post-menopause, while OC risk is reduced by full-term pregnancies during former fertile life. Herein, we investigate how parity history modulates transcriptome related such declining pool systemic inflammation in reproductively-aged mice. Female C57BL/6...

Ovarian cancer (OC) incidence and mortality peaks at post-menopause while OC risk is either reduced by parity or increased nulliparity during fertile life. The long-term effect of on ovarian gene expression largely unknown. In this study, we describe a bioinformatic/data-mining analysis 112 coding genes upregulated in the aged nulliparous (NP) mouse ovary compared to multiparous one as reference. Canonical ontology pathway analyses indicated pro-oxidant, xenobiotic-like state accompanied...

Breast cancer is the most common malignancy among women. Chilean studies reveal that this presents third highest mortality rate. A family history of breast one major risk factors for development disease. BRCA1 and BRCA2 are two main hereditary susceptibility genes, mutations in these genes related to inherited cancer. In specific populations only some have been found be associated with susceptibility. The purpose study was establish frequency 5382insC (BRCA1) 6174delT (BRCA2) germline 382...

Community-acquired pneumonia (CAP) is a worldwide cause of morbidity and mortality. Immunoglobulins (Igs) B cells quantification studies in CAP are few show discrepancies. Serum IgA acts as powerful natural anti-inflammatory factor, but its role the has not yet been defined. The highly sensitive xMAP Luminex technique allows better immunoglobulins quantification. aim this study was to analyze relation between clinical severity circulating Igs adults with CAP.Igs (M, A, G1, G2, G3, G4) were...

To identify clinical parameters in association with human papilloma virus (HPV) genotypes and histopathology diagnosis HIV-positive patients external condylomata acuminata (ECA), 400 Chilean were included the study. Forty-seven presented ECA. Clinical socio demographic data recorded. Histopathology study HPV linear array genotyping assay performed. Intraepithelial neoplasia (IEN) grade 2 or 3 was found 8.5% of patients, associated to HPV-16. Patients mainly single, MSM, history sexually...

Human adenoviruses are one of the most important pathogens detected in acute respiratory diseases pediatrics and immunocompromised patients. In 1953, Wallace Rowe described it for first time oropharyngeal lymphatic tissue. To date, more than 85 types HAdV have been described, with different cellular tropisms. They can cause gastrointestinal symptoms, even urinary tract inflammation, although infections asymptomatic. However, there is a population at risk that develop serious lethal...