A5034975966- Cell Image Analysis Techniques
- Animal testing and alternatives
- bioluminescence and chemiluminescence research
- Computational Drug Discovery Methods
- Pluripotent Stem Cells Research
- Molecular Biology Techniques and Applications
- 3D Printing in Biomedical Research
- Neuroscience and Neuropharmacology Research
- Gene expression and cancer classification
- Pesticide Exposure and Toxicity
- Carcinogens and Genotoxicity Assessment
- Effects and risks of endocrine disrupting chemicals
- Anesthesia and Neurotoxicity Research
- Epigenetics and DNA Methylation
- Flame retardant materials and properties
- Single-cell and spatial transcriptomics
- Polydiacetylene-based materials and applications
- Neuroendocrine regulation and behavior
- Antimicrobial Peptides and Activities
- Environmental Chemistry and Analysis
- Renal and related cancers
- Photoreceptor and optogenetics research
- Bacillus and Francisella bacterial research
- Retinoids in leukemia and cellular processes
- Digital Imaging for Blood Diseases
Environmental Protection Agency
2019-2025
Helmholtz Centre for Environmental Research
2023-2025
Oak Ridge Associated Universities
2019-2025
Research Triangle Park Foundation
2022-2025
Oak Ridge Institute for Science and Education
2019-2024
University of Konstanz
2015-2019
University of Zurich
2014
Fish acute toxicity testing is used to inform environmental hazard assessment of chemicals. In silico and in vitro approaches have the potential reduce number fish increase efficiency generating data for assessing ecological hazards. Here, two bioactivity assays were adapted use high-throughput chemical screening. First, a miniaturized version Organisation Economic Co-operation Development (OECD) test guideline 249 plate reader-based assay RTgill-W1 cells was developed. Second, Cell Painting...
Abstract 6PPD-quinone (N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine quinone), a transformation product of the antiozonant 6PPD (N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine) is likely causative agent coho salmon (Oncorhynchus kisutch) pre-spawn mortality. Stormwater runoff transports into freshwater streams, rapidly leading to neurobehavioral, respiratory distress, and rapid mortality in laboratory exposed salmon, but causing no many laboratory-tested species. Given this...
Halogen-free organophosphorus flame retardants are considered as replacements for the phased-out class of polybrominated diphenyl ethers (PBDEs). However, toxicological information on new is still limited. Based their excellent retardation potential, we have selected three novel 9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide (DOPO) derivatives and assessed profile using a battery in vitro test systems order to provide before large-scale production use. PBDE-99, applied reference...
Cell Painting is a high-throughput phenotypic profiling assay that uses fluorescent cytochemistry to visualize variety of organelles and high-content imaging derive large number morphological features at the single-cell level. Most studies have used U-2 OS cell line for chemical or functional genomics screening. The can be with many other human-derived types, given based on use fluoroprobes label are present in most (if not all) human cells. Questions remain, however, regarding optimization...
Migration of neural crest cells (NCCs) is one the pivotal processes human fetal development. Malformations arise if NCC migration and differentiation are impaired genetically or by toxicants. In currently available test systems for inhibition (MINC), manual generation a cell-free space results in extreme operator dependencies, limits throughput. Here new format was established. The assay avoids scratching plating around commercially circular stopper. Removal stopper barrier after cell...
Autism spectrum disorder (ASD) is heritable and neurodevelopmental with unknown causes. The serotonergic oxytocinergic systems are of interest in autism for several reasons: (i) Both implicated social behavior, abnormal levels serotonin oxytocin have been found people ASD; (ii) treatment selective reuptake inhibitors can yield improvements; (iii) previous association studies linked the transporter (SERT; SLC6A4), receptor 2A (HTR2A), (OXTR) genes ASD. We examined their high functioning (HFA)...
Phenotypic profiling assays are untargeted screening that measure a large number (hundreds to thousands) of cellular features in response stimulus and often yield diverse unanticipated profiles phenotypic effects, leading challenges distinguishing active from inactive treatments. Here, we compare variety different strategies for hit identification imaging-based using previously published Cell Painting data set. Hit based on multiconcentration analysis involve curve fitting at several levels...
Abstract Background Location-specific patterns of regulated and non-regulated disinfection byproducts (DBPs) were detected in tap water samples the Barcelona Metropolitan Area. However, it remains unclear if DBPs together with undetected DPBs organic micropollutants can lead to mixture effects drinking water. Objective To evaluate neurotoxicity, oxidative stress response cytotoxicity 42 samples, 6 treated activated carbon filters, 5 reverse osmosis 9 bottled waters. compare measured extracts...
Human cell-based toxicological assays have been used successfully to detect known toxicants, and distinguish them from negative controls. However, there is at present little experience on how deal with hits screens of compounds yet unknown hazard. As a case study this issue, we characterized human interferon-beta (IFNβ) as potential developmental toxicant affecting neural crest cells (NCC). The protein was identified hit during screen clinically drugs in the 'migration inhibition crest'...
Studies in vivo rodent models have been the accepted approach by regulatory agencies to evaluate potential developmental neurotoxicity (DNT) of chemicals for decades. These studies, however, are inefficient and cannot meet demand thousands that need be assessed DNT hazard. As such, several vitro new methods (NAMs) developed circumvent limitations these traditional studies. The NAMs, some which utilize human-derived cell models, intended employed a testing battery approach, each focused on...
Abstract While there are many methods to quantify the synthesis, localization, and pool sizes of proteins DNA during physiological responses toxicological stress, only few approaches allow following fate carbohydrates. One them is metabolic glycoengineering (MGE), which makes use chemically modified sugars (CMS) that enter cellular biosynthesis pathways leading glycoproteins glycolipids. The CMS can subsequently be coupled (via bio-orthogonal chemical reactions) tags quantifiable by...
A vast amount of toxicological data can be obtained from feature analysis cells treated in vitro. However, this requires microscopic image segmentation cells. To end, we propose a new strategy, namely Supervised Normalized Cut Segmentation (SNCS), to segment that partially overlap and have large curved edges. SNCS approach is machine learning based method, where loosely annotated images are used first train optimise parameters, then the optimal parameters inserted into process. Furthermore,...
Migration of neural crest cells (NCCs) is one the pivotal processes human fetal development. Malformations arise if NCC migration and differentiation are impaired genetically or by toxicants. In currently available test systems for inhibition (MINC), manual generation a cell-free space results in extreme operator dependencies, limits throughput. Here new format was established. The assay avoids scratching plating around commercially circular stopper. Removal stopper barrier after cell...