- Plant Gene Expression Analysis
- Lignin and Wood Chemistry
- Biochemical and biochemical processes
- Genetic Mapping and Diversity in Plants and Animals
- Genetics and Plant Breeding
- Biofuel production and bioconversion
- Wheat and Barley Genetics and Pathology
- Spectroscopy and Chemometric Analyses
- Plant biochemistry and biosynthesis
- Genomics, phytochemicals, and oxidative stress
- Nitrogen and Sulfur Effects on Brassica
- Thermochemical Biomass Conversion Processes
- GABA and Rice Research
- Botanical Research and Chemistry
- Plant Disease Resistance and Genetics
- Phytochemicals and Antioxidant Activities
- Plant-Microbe Interactions and Immunity
- Phytase and its Applications
- Meat and Animal Product Quality
- Seed Germination and Physiology
- Plant and animal studies
- Bioenergy crop production and management
- Food composition and properties
- Potato Plant Research
- Selenium in Biological Systems
Pepsi (United States)
2019-2022
PepsiCo (United Kingdom)
2019
Kansas State University
2017
Purdue University West Lafayette
2013-2016
South University
2016
Puccinia graminis f. sp. tritici (Pgt) causes wheat stem rust, a devastating fungal disease. The Sr35 resistance gene confers immunity against this pathogen's most virulent races, including Ug99. We used comparative whole-genome sequencing of chemically mutagenized and natural Pgt isolates to identify named AvrSr35 that is required for avirulence. encodes secreted protein capable interacting with triggering the immune response. show origin on associated nonfunctionalization by insertion...
Modifying lignin composition and structure is a key strategy to increase plant cell wall digestibility for biofuel production. Disruption of the genes encoding both cinnamyl alcohol dehydrogenases (CADs), including CADC CADD, in Arabidopsis thaliana results atypical incorporation hydroxycinnamaldehydes into lignin. Another change downregulation or overexpression ferulate 5-hydroxylase (F5H), which lignins enriched guaiacyl syringyl units, respectively. Here, we combined these approaches...
Plants produce an array of metabolites (including lignin monomers and soluble UV-protective metabolites) from phenylalanine through the phenylpropanoid biosynthetic pathway. A subset plants, including many related to Arabidopsis thaliana, synthesizes glucosinolates, nitrogen- sulfur-containing secondary that serve as components a plant defense system deters herbivores pathogens. Here, we report thaliana reduced epidermal fluorescence5 (ref5-1) mutant, identified in screen for plants with...
Lignin is an abundant phenylpropanoid polymer produced by the oxidative polymerization of p-hydroxycinnamyl alcohols (monolignols). Lignification, i.e., deposition lignin, a defining feature secondary cell wall formation in vascular plants, and provides important mechanism for their disease resistance; however, many aspects lignification process remain unclear partly because lack suitable imaging methods to monitor vivo. In this study, set monolignol analogs γ-linked fluorogenic...
Impact of engineered lignin composition on biomass recalcitrance.
Phenylpropanoids are phenylalanine-derived specialized metabolites and include important structural components of plant cell walls, such as lignin hydroxycinnamic acids, well ultraviolet visible light-absorbing pigments, hydroxycinnamate esters (HCEs) anthocyanins. Previous work has revealed a remarkable degree plasticity in HCE biosynthesis, that most Arabidopsis (Arabidopsis thaliana) mutants with blockages the pathway simply redirect carbon flux to atypical HCEs. In contrast, ferulic acid...
Floral attraction traits can significantly affect pollinator visitation patterns, but adaptive evolution of these may be constrained by correlations with other traits. In some cases, molecular pathways contributing to floral are well characterized, offering the opportunity explore loci potentially underlying variation among individuals. Here, we quantify range in UV patterning (i.e. 'bulls-eye nectar guides) crop and wild accessions Brassica rapa. We then use experimental crosses examine...
Abstract Maize ( Zea mays L.) is a multi‐purpose row crop grown worldwide, which, over time, has often been bred for increased yield at the detriment of lower composition grain quality. Some knowledge genetic factors that affect quality traits discovered through study classical maize mutants; however, much underlying control these and interaction between remains unknown. To better understand variation exists compositional in maize, we evaluated 501 diverse temperate inbred lines five unique...
ABSTRACT Maize ( Zea mays L.) masa (dough or flour that is soaked and cooked in an alkaline solution the nixtamlization process) based products have been consumed by humans worldwide for thousands of years. Still, there not a comprehensive understanding chemical physical properties maize contribute to quality. Starches proteins affect processing but are seldom discussed holistic way understand their individual combined effects on production, particularly context entire food system from...
Hydroxycinnamic acids, such as p-coumaric acid and ferulic acid, are a major class of compounds derived from the phenylpropanoid pathway. These widely conserved in plants primarily accumulate secondary cell wall. They serve important structural components that contribute to overall strength rigidity plant walls also potent antioxidants valued for nutritional consumption. This protocol describes method analyzing hydroxycinnamic acids released after incubation under alkaline conditions.
ABSTRACT Maize ( Zea mays L.) is a multi-purpose row crop grown worldwide, which overtime has often been bred for increased yield at the detriment of lower composition grain quality. Some knowledge genetic factors that affect quality traits discovered through study classical maize mutants. However, much underlying architecture controlling these and interaction between remains unknown. To better understand variation exists compositional in maize, we evaluated 501 diverse temperate inbred...
Field pennycress (Thlaspi arvense L.) is a winter annual oilseed undergoing development as cash cover crop for the Upper Midwest. While it provides ecosystem benefits when planted between conventional rotations, there are few end-uses wild pennycress. Pennycress high in oil and protein, current efforts aim to improve seed composition generate environmentally sustainable food sources. Similar Arabidopsis thaliana rape, rich glucosinolates, which limits palatability utility. This study aimed...
Consumption of corn-based foods is increasing in the United States and globally. Little known about changes kernel compositional profiles, including levels acrylamide precursors (e.g. asparagine reducing sugars), when corn kernels are cooked a process called nixtamalization. The objectives this study were to investigate variation raw starting materials, general during nixtamalization, genotype specific patterns change for process. A panel 120 genotypes subject small-scale bench top cooking...
ABSTRACT Lack of high throughput phenotyping systems for determining moisture content during the maize nixtamalization cooking process has led to difficulty in breeding this trait. This study provides a throughput, quantitative measure kernel based on NIR scanning uncooked kernels. Machine learning was utilized develop models combination spectra and determined from scaled-down benchtop cook method. A linear support vector machine (SVM) model with Spearman’s rank correlation coefficient 0.852...