A5082997958- Alzheimer's disease research and treatments
- Neuroscience and Neural Engineering
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Photoacoustic and Ultrasonic Imaging
- Optical Imaging and Spectroscopy Techniques
- Cell Image Analysis Techniques
- Cholinesterase and Neurodegenerative Diseases
- Electrochemical Analysis and Applications
- Molecular Communication and Nanonetworks
- Neurogenesis and neuroplasticity mechanisms
- Pluripotent Stem Cells Research
- Cellular transport and secretion
- Photoreceptor and optogenetics research
- Lysosomal Storage Disorders Research
- Amyotrophic Lateral Sclerosis Research
- Genetic Neurodegenerative Diseases
- Mass Spectrometry Techniques and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Stress Responses and Cortisol
- Advanced Cellulose Research Studies
- Diet and metabolism studies
- EEG and Brain-Computer Interfaces
University of Cambridge
2015-2025
MRC Laboratory of Molecular Biology
2021-2024
University College London
2015
National Hospital for Neurology and Neurosurgery
2015
Mutations in the charged multivesicular body protein 2B (CHMP2B) cause frontotemporal dementia (FTD). We report that mice which express FTD-causative mutant CHMP2B at physiological levels develop a novel lysosomal storage pathology characterised by large neuronal autofluorescent aggregates. The aggregates are an early and progressive occur 3 months of age increase both size number over time. These not observed expressing wild-type CHMP2B, or non-transgenic controls, indicating they specific...
Advanced optical imaging techniques address important biological questions in neuroscience, where structures such as synapses are below the resolution limit of a conventional microscope. At same time, microelectrode arrays (MEAs) indispensable understanding language neurons. Here, authors show transparent MEAs capable recording action potentials from neurons and compatible with advanced microscopy. The electrodes made conducting polymer poly(3,4-ethylenedioxythiophene) doped polystyrene...
<ns3:p>Techniques for calcium imaging were first demonstrated in the mid-1970s, whilst tools to analyse these markers of cellular activity are still being developed and improved today. For image analysis, custom within labs until relatively recently, software packages not widely available between researchers. We will discuss some most popular methods analysis that now describe why protocols so effective. also newest innovations field likely benefit researchers, particularly as is often an...
Disrupting maladaptive memories may provide a novel form of treatment for neuropsychiatric disorders, but little is known about the neurochemical mechanisms underlying induction lability, or destabilization, retrieved consolidated memory. Destabilization has been theoretically linked to violation expectations during memory retrieval, which, in turn, suggested correlate with prediction error (PE). It well-established that PE correlates dopaminergic signaling limbic forebrain structures are...
Advances in experimental and computational technologies continue to grow rapidly provide novel avenues for the treatment of neurodegenerative disorders. Despite this, there remain only a handful drugs that have shown success late-stage clinical trials Tau-associated The most commonly prescribed treatments are symptomatic such as cholinesterase inhibitors N-methyl-D-aspartate receptor blockers were approved use Alzheimer's disease. As diagnostic screening can detect disorders at earlier time...
Microelectrode arrays (MEAs) permit recordings with high electrode counts, thus generating complex datasets that would benefit from precise neuronal spike sorting for meaningful data extraction. Nevertheless, conventional methods face limitations in recognizing diverse shapes. Here, we introduce PseudoSorter, which uses self-supervised learning techniques, a density-based pseudolabeling strategy, and an iterative fine-tuning process to enhance accuracy. Through benchmarking, demonstrate the...
The three-dimensional imaging of mesoscopic samples with Optical Projection Tomography (OPT) has become a powerful tool for biomedical phenotyping studies. OPT uses visible light to visualize the 3D morphology large transparent samples. To enable wider application OPT, we present OptiJ, low-cost, fully open-source system capable specimens up 13 mm tall and 8 deep 50 µm resolution. OptiJ is based on off-the-shelf, easy-to-assemble optical components an ImageJ plugin library data...
Abstract Background Stem cell-based therapies for neurodegenerative diseases like Parkinson’s disease are a promising approach in regenerative medicine and now moving towards early stage clinical trials. However, number of challenges remain including the ability to grow stem cells vitro on 3-dimensional scaffold, as well their loss, by leakage or cell death, post-implantation. These issues could, however, be helped through use scaffolds that support growth differentiation both vivo. The...
Techniques for calcium imaging were first achieved in the mid-1970s, whilst tools to analyse these markers of cellular activity are still being developed and improved. For image analysis, custom within labs until relatively recently, software packages not widely available between researchers. We will discuss some most popular, alongside our preferred, methods analysis that now describe why protocols so effective. also newest innovations field likely benefit researchers, particularly as is...
Memory naturally declines as we age, but the rapid loss of memory can be distressing for people living with Alzheimer’s disease (AD). How memories are formed and retrieved in brain is not fully understood; it thought to require plasticity synapses connecting neurons a network engram cells. Plasticity may occur either through changes volume location molecules organelles within synapse, or gross structural synapses. do many mechanisms required learning memory, such concentrations cytoskeletal...
Abstract Extracellular recordings of neuronal activity constitute a powerful tool for investigating the intricate dynamics neural networks and individual neurons. Microelectrode arrays (MEAs) allow with high electrode count, ranging from 10s to 1000s, generating extensive datasets information. Furthermore, MEAs capture extracellular field potentials cultured cells, resulting in highly complex signals that necessitate precise spike sorting meaningful data extraction. Nevertheless,...
Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) are both devastating neurodegenerative conditions. Despite affecting different regions of the nervous system (FTD primarily frontal temporal lobes, whilst ALS presents with motor neuron loss), there is significant overlap between these conditions in terms genetics, pathology, disease mechanisms, they therefore often grouped as a spectrum symptoms under heading FTD/ALS (Abramzon et al., 2020). Significantly, currently no...
Optogenetics has revolutionised research in cell biology over the past 15 years, yet devices that can effectively stimulate cells using light are often costly and specifically designed for a single experimental set-up with little flexibility. Our novel 'OptoGenie' stimulation device be conveniently transferred between culture incubators long-term stimulation, electrophysiology rigs patch-clamp recordings, optical microscopes fluorescence imaging of cells. The modular design offers...
The three-dimensional imaging of mesoscopic samples with Optical Projection Tomography (OPT) has become a powerful tool for biomedical phenotyping studies. OPT uses visible light to visualize the 3D morphology large transparent samples. To enable wider application OPT, we present OptiJ, low-cost, fully open-source system capable specimens up 13 mm tall and 8 deep 50 μm resolution. OptiJ is based on off-the-shelf, easy-to-assemble optical components an ImageJ plugin library data...