A5032059940- Electrochemical sensors and biosensors
- Biofuel production and bioconversion
- Microbial Fuel Cells and Bioremediation
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Electrochemical Analysis and Applications
- Electrocatalysts for Energy Conversion
- Catalysis for Biomass Conversion
- Supercapacitor Materials and Fabrication
- Advanced Cellulose Research Studies
- biodegradable polymer synthesis and properties
- CO2 Reduction Techniques and Catalysts
- Biochemical and Molecular Research
- Enzyme Production and Characterization
- Advanced biosensing and bioanalysis techniques
- Advanced battery technologies research
- Advanced Nanomaterials in Catalysis
- Diamond and Carbon-based Materials Research
- Graphene research and applications
- MXene and MAX Phase Materials
- Metalloenzymes and iron-sulfur proteins
- Analytical Chemistry and Sensors
- Protist diversity and phylogeny
- Microbial metabolism and enzyme function
- Diet, Metabolism, and Disease
South China University of Technology
2025
Zhejiang University of Technology
2023-2025
Tianjin Institute of Industrial Biotechnology
2016-2024
Chinese Academy of Sciences
2016-2024
University of Chinese Academy of Sciences
2019-2024
Nanjing University of Chinese Medicine
2024
Tianjin Economic-Technological Development Area
2021-2023
Singapore Institute of Manufacturing Technology
2022
Intelligent Synthetic Biology Center
2022
Virginia Tech
2008-2014
While many pretreatments attempt to improve the enzymatic digestibility of biomass by removing lignin, this study shows that improving surface area accessible cellulase is a more important factor for achieving high sugar yield. Here we compared pretreatment switchgrass two methods, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) soaking in aqueous ammonia (SAA). Following pretreatment, hydrolysis was conducted at loadings, 15 filter paper units (FPU)/g...
Liberation of fermentable sugars from recalcitrant biomass is among the most costly steps for emerging cellulosic ethanol production. Here we compared two pretreatment methods (dilute acid, DA, and cellulose solvent organic lignocellulose fractionation, COSLIF) corn stover. At a high cellulase loading [15 filter paper units (FPUs) or 12.3 mg per gram glucan], glucan digestibilities stover pretreated by DA COSLIF were 84% at hour 72 97% 24, respectively. low (5 FPUs glucan), digestibility...
Cellulose accessibilities of a set hornified lignocellulosic substrates derived by drying the never dried pretreated sample and differently lodgepople pine substrates, were evaluated using solute exclusion protein adsorption methods. Direct measurements cellulase onto cellulose surface also carried out an in situ UV-Vis spectrophotometric technique. The measured cellulose-binding module (CBM)-containing green fluorescent (TGC) methods correlate well for both sets samples. substrate enzymatic...
Abstract The switchgrass (SG) samples pretreated by cellulose solvent‐ and organic solvent‐based lignocellulose fractionation were characterized enzymatic hydrolysis, substrate accessibility assay, scanning electron microscopy, X‐ray diffraction (XRD), cross polarization/magic angle spinning (CP/MAS) 13 C nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FTIR). Glucan digestibility of the SG was 89% at hour 36 one filter paper unit cellulase per gram glucan....
Abstract Enzymatic electrosynthesis has gained more and interest as an emerging green synthesis platform, particularly for the fixation of CO 2 . However, simultaneous utilization a nitrogenous molecule enzymatic value‐added products never been reported. In this study, we constructed in vitro multienzymatic cascade based on reductive glycine pathway demonstrated electrocatalytic system that allowed conversion NH 3 sole carbon nitrogen sources to synthesize glycine. Through effective coupling...
Abstract The transformation of graphite into diamond (2–10 nm) at ordinary pressure by monodispersed Ta atoms was recently reported, while the effects concentration on transition process remain obscure. Here, regulating wire treatment time, as well annealing time and temperature, larger grians (5–20 are successfully synthesized, to is revealed vary with concentration. Specifically, short treatments (5–10 min) induce form a “circle” structure transforms directly after annealing. Long (15–25...
Efficient liberation of fermentable soluble sugars from lignocellulosic biomass waste not only decreases solid handling but also produces value-added biofuels and biobased products. Industrial hemp, a special economic crop, is cultivated for its high-quality fibers high-value seed oil, hollow stalk cords (hurds) are cellulosic waste. The cellulose-solvent-based lignocellulose fractionation (CSLF) technology has been developed to separate components under modest reaction conditions (Zhang,...
Effective hydrolysis of pretreated lignocellulose mediated by cellulase requires an in-depth understanding adsorption and desorption. Here we developed a simple method for determining the adsorbed on cellulosic materials or lignocellulose, which involves (i) in presence 10 M NaOH at 121 °C 20 min, (ii) ninhydrin assay amino acids released from hydrolyzed cellulase. The major lignocellulosic components (i.e., cellulose, hemicellulose, lignin) did not interfere with assay. A number desorption...
Cost-effective biological saccharification of nonfood lignocellulosic biomass is vital to the establishment a carbohydrate economy. Phragmites australis (common reed) regarded as an invasive perennial weed with productivity up 18−28 tons dry weight per acre year. We applied cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) stems leaves optimized pretreatment conditions (e.g., temperature, reaction time, moisture content) through response surface methodology...
Cellulosic biomass is the earth's most abundant renewable resource, which considered to be a promising feedstock for manufacturing biofuels and biochemicals. In this study, stoichiometric enzymatic phosphorolysis of cellulosic biochemicals or by in vitro synthetic biosystems was designed. Three cascade phosphorolytic enzymes, cellodextrin phosphorylase, cellobiose polyphosphate-dependent glucokinase, were used biotransformation cellodextrins high-energy phosphorylated sugars (that is,...
Abstract Marine microbial ecosystems can be viewed as a huge ocean-battery charged by solar energy. It provides model for fabricating bio-solar cell, bioelectrochemical system that converts light into electricity. Here, we fabricate cell consisting of four-species community mimicking the ecological structure marine ecosystems. We demonstrate such primary producer, degrader, and ultimate consumers is essential achieving high power density stability. Furthermore, assembled spatial-temporally...
Nicotinamide adenine dinucleotide (NAD(P)+)-dependent oxidoreductases have been widely employed as biocatalysts for numerous applications, such in vitro biomanufacturing and biosensors. The application of biomimetic nicotinamide coenzymes (BNCs) an enzymatic redox cascade constitutes a promising alternative that can eliminate the need expensive natural NAD(P)+ coenzymes. Herein, we demonstrated coenzyme engineering glucose-6-phosphate dehydrogenase from Zymomonas mobilis (ZmG6PDH) enhanced...
While most in vitro synthetic biology projects are usually used for the purposes of basic science research or formation high-value products, cell-free pathway biotransformation (SyPaB), which can implement complicated biochemical reactions by assembly numerous enzymes and coenzymes, would be low-cost biomanufacturing. In this article, we present bottom-up design principles SyPaB from building blocks (enzymes and/or immobilized enzymes) to modules, such as NAD(P)H regeneration, consumption,...