A5005468831- Microbial Natural Products and Biosynthesis
- Microbial Metabolism and Applications
- Phytochemicals and Antioxidant Activities
- CRISPR and Genetic Engineering
- Plant biochemistry and biosynthesis
- Enzyme Production and Characterization
- Plant Gene Expression Analysis
- Phytochemical compounds biological activities
- Essential Oils and Antimicrobial Activity
- Phytochemistry and Biological Activities
Huazhong Agricultural University
2023-2024
Northwest A&F University
2020-2021
Abstract Monascus pilosus has been used to produce lipid-lowering drugs rich in monacolin K (MK) for a long period. Genome mining reveals there are still many potential genes worth be explored this fungus. Thereby, efficient genetic manipulation tools will greatly accelerate progress. In study, we firstly developed the protocol prepare protoplasts recipient of CRISPR/Cas9 system. Subsequently, vector and donor DNA were co-transformed into recipients (10 6 protoplasts/mL) 60–80 transformants...
Monascus spp. can produce a variety of beneficial metabolites widely used in food and pharmaceutical industries. However, some species contain the complete gene cluster responsible for citrinin biosynthesis, which raises our concerns about safety their fermented products. In this study, Mrhos3, encoding histone deacetylase (HDAC), was deleted to evaluate its effects on production mycotoxin (citrinin) edible pigments as well developmental process ruber M7. The results showed that absence...
Chaenomeles species are used for both ornamental decoration and medicinal purposes. In order to have a better understanding of the flavonoid profile Chaenomeles, petals four species, including japonica (RB), speciose (ZP), sinensis (GP), cathayensis (MY), were selected as experimental material. The total content GP was found be highest, followed by MY, ZP, RB. total, 179 metabolites (including 49 flavonols, 46 flavonoids, 19 flavone C-glycosides, 17 procyanidins, 15 anthocyanins, 10...