A5102851603- Cancer Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Amino Acid Enzymes and Metabolism
- Virus-based gene therapy research
- Neuroblastoma Research and Treatments
- Carbohydrate Chemistry and Synthesis
- Bacteriophages and microbial interactions
- Bladder and Urothelial Cancer Treatments
- Hippo pathway signaling and YAP/TAZ
- Cancer-related Molecular Pathways
- Seaweed-derived Bioactive Compounds
- Cardiac electrophysiology and arrhythmias
- Sulfur Compounds in Biology
- Glycosylation and Glycoproteins Research
- Occupational and environmental lung diseases
- Cell death mechanisms and regulation
- Plant Molecular Biology Research
- Metal complexes synthesis and properties
- Fibroblast Growth Factor Research
- Plant Parasitism and Resistance
- Protein Kinase Regulation and GTPase Signaling
- Enzyme Production and Characterization
- Genomics and Phylogenetic Studies
- Polyamine Metabolism and Applications
- Enzyme Structure and Function
Max Planck Institute of Colloids and Interfaces
2024
Freie Universität Berlin
2024
Chungnam National University
2024
Bowdoin College
2021-2022
Queen Mary University of London
2010-2016
Molecular Oncology (United States)
2016
San Diego State University
2016
AGH University of Krakow
2013
Southern Taiwan University of Science and Technology
2013
Wellcome Trust
2013
MAX2 (for MORE AXILLARY GROWTH2) has been shown to regulate diverse biological processes, including plant architecture, photomorphogenesis, senescence, and karrikin signaling. Although is a smoke-derived abiotic signal, role for in stress response pathways least investigated. Here, we show that the max2 mutant strongly hypersensitive drought compared with wild-type Arabidopsis (Arabidopsis thaliana). Stomatal closure of was less sensitive abscisic acid (ABA) than wild type. Cuticle thickness...
Preclinical studies show that arginine deprivation is synthetically lethal in argininosuccinate synthetase 1 (ASS1)-negative cancers, including mesothelioma. The role of the arginine-lowering agent pegylated deiminase (ADI-PEG20) has not been evaluated a randomized and biomarker-driven study among patients with cancer.
Targeted therapies have yet to significant impact on the survival of patients with bladder cancer. In this study, we focused urea cycle enzyme argininosuccinate synthetase 1 (ASS1) as a therapeutic target in cancer, based our discovery prognostic and functional import ASS1 setting. expression status tumors from 183 Caucasian 295 Asian was analyzed, along its hypothesized association clinicopathologic features, including tumor size invasion. Furthermore, genetics, biology, implications loss...
Yes-associated protein (YAP) regulates DNA damage and chemosensitivity, as well functioning a pro-growth, cell size regulator. For both of its roles, regulation by phosphorylation is crucial. We undertook an in vitro screen to identify novel YAP kinases discover new signaling pathways better understand YAP's function. identified JNK1 JNK2 robust kinases, mapped multiple sites phosphorylation. Using inhibitors siRNA, we showed that JNK specifically phosphorylates endogenous number types. show...
Tumours lacking argininosuccinate synthetase-1 (ASS1) are auxotrophic for arginine and sensitive to amino-acid deprivation. Here, we investigated the role of ASS1 as a biomarker response arginine-lowering agent, pegylated deiminase (ADI-PEG20), in lymphoid malignancies. Although protein was largely undetectable normal malignant tissues, frequent hypermethylation promoter observed specifically latter. A good correlation between methylation, low mRNA, absence expression sensitivity ADI-PEG20...
Fucoidan, a sulfated polysaccharide found in algae, plays central role marine carbon sequestration and exhibits wide array of bioactivities. However, the molecular diversity structural complexity fucoidan hinder precise structure–function studies. To address this, we present an automated method for generating well-defined linear branched α-fucan oligosaccharides. Our syntheses include oligosaccharides with up to 20 cis-glycosidic linkages, diverse branching patterns, 11 sulfate monoesters....
The p53 family member p63 has been shown to be critical for growth, proliferation and chemosensitivity. Here we demonstrate that the c-Abl tyrosine kinase phosphorylates widely expressed ΔNp63α isoform identify multiple sites by mass spectrometry in vitro vivo. Phopshorylation results greater protein stability of both ectopically endogenous ΔNp63α. phosphorylation induces its binding Yes-associated (YAP) silencing YAP siRNA reduces c-Abl-induced increase levels. We further show cisplatin...
Fucoidan, a sulfated polysaccharide found in algae, occupies central yet enigmatic role marine carbon sequestration and exhibits wide array of bioactivities. However, the inherent molecular diversity structural complexity fucoidan hinders precise structure-function studies. To address this, we present rapid reproducible automated synthesis method for generating well-defined linear branched α-fucan oligosaccharides. Our syntheses include oligosaccharides with up to 20 cis-glycosidic linkages,...
Bacterial cell envelope glycans are compelling antibiotic targets as they critical for strain fitness and pathogenesis yet virtually absent from human cells. However, systematic study perturbation of bacterial remains challenging due to their utilization rare deoxy amino l-sugars, which impede traditional glycan analysis not readily available natural sources. The development chemical tools is a crucial step toward understanding altering these biomolecules. Here we report an expedient...
One proposed strategy to suppress the proliferation of imatinib-resistant cells in chronic myeloid leukemia (CML) is inhibit key proteins downstream Bcr-Abl. The PI3K/Akt pathway activated by Bcr-Abl and specifically required for growth CML cells. To identify targets this pathway, we undertook a proteomic screen identified several that differentially bind 14-3-3, dependent on kinase activity. An siRNA candidates selected bioinformatics analysis reveals cold-shock domain protein A (CSDA),...
Diphenyleneiodonium (DPI) has been widely used as an inhibitor of NADPH oxidase (Nox) to discover its function in cardiac myocytes under various stimuli. However, the effects DPI itself on Ca
Abstract Malignant pleural mesothelioma (MPM) is a devastating asbestos-related malignancy that increasing in many countries worldwide with few systemic treatment options beyond platinum and antifolate chemotherapy. Deficiency of the arginine biosynthetic enzyme argininosuccinate synthetase (ASS1) occurs up to 50% MPM cell lines primary tumors being validated as biomarker patients treated arginine-depleting agent, pegylated deiminase (ADI-PEG20). To understand role ASS1 loss effect ADI-PEG20...
7587 Background: Arginine is a key amino acid for tumorigenesis modulating diverse array of metabolic pathways, from synthesis proteins, NO and polyamines to nucleotide turnover mTOR signalling. Tumors deficient in the urea cycle enzyme, argininosuccinate synthetase 1 (ASS1), are especially sensitive deprivation exogenous arginine using drug pegylated deiminase (ADI-PEG20, Polaris Group, US). Previously, we have reported changes induced by ADI treatment panel ASS1-deficient bladder cancer...
Abstract Tumors deficient in argininosuccinate synthetase-1 (ASS1), a key enzyme involved arginine synthesis, are auxotrophic for and sensitive to amino acid deprivation. Currently, several trials testing the arginine-depleting agent, pegylated deiminase (ADI-PEG20) patients with cancer, including randomised phase II multicenter UK study ASS1-deficient mesothelioma (NCT01279967). Here, we sought identify pathways mechanism of action ADI-PEG20 using panel malignant (MPM) cell lines as our...
Abstract Background Arginine is an important amino acid for tumor cell growth and development. Argininosuccinate synthetase 1 (ASS1) a key enzyme required biosynthesis of arginine. Preclinically, ASS1-deficient cells are particularly sensitive to arginine depletion, randomised trials exploring this strategy in progress hepatocellular carcinoma mesothelioma using the drug pegylated deiminase (ADI-PEG20). In study, we determined metabolic changes induced by ADI-PEG20 treatment panel bladder...
<p>PDF file - 408K, Pyrosequencing data of bladder cancer cell lines and primary samples.</p>
<p>PDF file - 65K</p>
<p>PDF file - 65K</p>