A5074547179- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- RNA modifications and cancer
- Viral Infections and Immunology Research
- Monoclonal and Polyclonal Antibodies Research
- Viral gastroenteritis research and epidemiology
- Cancer-related molecular mechanisms research
- Neurogenesis and neuroplasticity mechanisms
- Genomics and Chromatin Dynamics
- Circular RNAs in diseases
- CAR-T cell therapy research
- Developmental Biology and Gene Regulation
- Virus-based gene therapy research
- Cancer, Hypoxia, and Metabolism
- RNA regulation and disease
- Protein Structure and Dynamics
- Olfactory and Sensory Function Studies
- Plant Virus Research Studies
- Chemical Synthesis and Analysis
- Sleep and Work-Related Fatigue
- PI3K/AKT/mTOR signaling in cancer
- Click Chemistry and Applications
- Advanced Fluorescence Microscopy Techniques
- Circadian rhythm and melatonin
- Biochemical Analysis and Sensing Techniques
Albany State University
2017-2022
University at Albany, State University of New York
2017-2022
Albany Research Institute
2019-2022
Purchase College
2019
Stanford University
2009-2017
Yale University
2004-2006
Howard Hughes Medical Institute
2004
University of Münster
1989
Idiopathic pulmonary arterial hypertension (PAH [IPAH]) is an insidious and potentially fatal disease linked to a mutation or reduced expression of bone morphogenetic protein receptor 2 (BMPR2). Because intravascular inflammatory cells are recruited in IPAH pathogenesis, we hypothesized that BMPR2 enhances production the potent chemokine granulocyte macrophage colony-stimulating factor (GM-CSF) response perturbation. When human artery (PA) endothelial deficient were stimulated with tumor...
More than 140 post-transcriptional modifications (PTMs) are known to decorate cellular RNAs, but their incidence, identity and significance in viral RNA still largely unknown. We have developed an agnostic analytical approach comprehensively survey PTMs on RNAs. Specifically, we used mass spectrometry analyze total isolated from cells infected with Zika virus, Dengue hepatitis C virus (HCV), poliovirus human immunodeficiency type 1. All five viruses significantly altered global PTM...
The effects of shifted working and sleeping phases on the diurnal blood pressure rhythm were investigated in 15 physically industrial shift workers at a slowly rotated three-shift system. Ambulatory 24-h monitoring was performed during morning night shifts. In two shifts mean identical. There no differences levels or periods between cycles. Diurnal fluctuations had equal amplitudes. Corresponding to lag period there phase difference 8h curves. At this lag, high correlation hourly values (r =...
The Ro autoantigen is a ring-shaped RNA-binding protein that binds misfolded RNAs in nuclei and proposed to function quality control. In the cytoplasm, noncoding RNAs, called Y inhibit access of other RNAs. also assists survival mammalian cells at least one bacterium after UV irradiation. mammals, undergoes dramatic localization changes irradiation, changing from mostly cytoplasmic predominantly nuclear. Here, we report second role regulate subcellular distribution Ro. A mutant does not bind...
Picornaviruses use internal ribosome entry sites (IRESs) to translate their genomes into protein. A typical feature of these IRESs is ability bind directly the eukaryotic initiation factor (eIF) 4G component eIF4F cap-binding complex. Remarkably, hepatitis virus (HAV) IRES requires eIF4E for its translation, but no mechanism has been proposed explain this. Here we demonstrate that regulates HAV IRES-mediated translation by two distinct mechanisms. First, binding eIF4G generates a...
Receptor for activated C kinase 1 (RACK1) is a eukaryote-specific ribosomal protein (RP) implicated in diverse biological functions. To engineer ribosomes specific fluorescent labeling, we selected RACK1 as target given its location on the small subunit and other properties. However, prior results suggested that has roles both off ribosome, such an exchange might be related to various cellular functions hinder our ability use stable tag ribosome. In addition, kinetics of spontaneous or any...
Significance Protein biosynthesis is most tightly controlled during translation initiation that involves numerous factors and regulatory proteins. This complexity confounds conventional biochemical methods. Single-molecule approaches are ideally suited to address such questions. However, their application hindered by the lack of fluorescently labeled components eukaryotic machinery. Here, we demonstrate an approach label human 40S ribosomal subunits. As extension this approach, used...
During mammalian development, gonadotropin-releasing-hormone-1 neurons (GnRH-1ns) migrate from the developing vomeronasal organ (VNO) into brain asserting control of pubertal onset and fertility. Recent data suggest that correct development olfactory ensheathing cells (OEC) is imperative for normal GnRH-1 neuronal migration. However, full ensemble molecular pathways regulate OEC remains to be fully deciphered. Loss-of-function transcription factor Gli3 known disrupt however, if plays a role...
Maternal mRNAs synthesized during oogenesis initiate the development of future generations. Some maternal are either somatic or germline determinants and must be translationally repressed until embryogenesis. However, translational repressors themselves temporally regulated. We used polar granule component (pgc), a Drosophila mRNA, to ask how transcripts while regulatory landscape is shifting. pgc, determinant, regulated throughout oogenesis. find that different conserved RNA-binding...
Sm-like (Lsm) proteins function in a variety of RNA-processing events. In yeast, the Lsm2-Lsm8 complex binds and stabilizes spliceosomal U6 snRNA, whereas Lsm1-Lsm7 functions mRNA decay. Here we report that third Lsm complex, consisting Lsm2-Lsm7 proteins, associates with snR5, box H/ACA snoRNA to guide site-specific pseudouridylation rRNA. Experiments which binding snR5 was reconstituted vitro reveal 3' end is critical for protein recognition. Glycerol gradient sedimentation sequential...
Abstract Failure to prevent accumulation of the non-canonical nucleotide inosine triphosphate (ITP) by pyrophosphatase (ITPase) during synthesis results in misincorporation into RNA and can cause severe fatal developmental anomalies humans. While biochemical activity ITPase is well understood, pathogenic basis deficiency molecular cellular consequences ITP remain cryptic. Here, we demonstrate that excess pool vitro transcription T7 polymerase-mediated luciferase RNA. In translation...
Protein synthesis by the ribosome is coordinated an intricate series of large-scale conformational rearrangements. Structural studies can provide information about long-lived states, however biological kinetics are controlled intervening free-energy barriers. While there has been progress describing energy landscapes bacterial ribosomes, very little known energetics rearrangements in eukaryotic systems. To address this topic, we constructed all-atom model with simplified and performed...
Abstract Most ribosomal proteins in Saccharomyces cerevisiae are encoded by two paralogs that additively produce the optimal protein level for cell growth. Nonetheless, deleting one paralog of most gene pairs results a variety phenotypes not observed when other is deleted. To determine whether paralog-specific associated with RPL7A or RPL7B stem from distinct functions different levels isoforms, coding region and introns paralog, including an intron-embedded snoRNA (small nucleolar RNA)...
Human translation initiation relies on the combined activities of numerous ribosome-associated eukaryotic factors (eIFs). The largest factor, eIF3, is an ∼800 kDa multiprotein complex that orchestrates a network interactions with small 40S ribosomal subunit, other eIFs, and mRNA, while participating in nearly every step initiation. How these take place during time course remains unclear. Here, we describe method for expression affinity purification fluorescently-tagged eIF3 from human cells....