A5034353395- Asthma and respiratory diseases
- IL-33, ST2, and ILC Pathways
- Inhalation and Respiratory Drug Delivery
- Allergic Rhinitis and Sensitization
- Neonatal Respiratory Health Research
- Immune Cell Function and Interaction
- Respiratory and Cough-Related Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Eosinophilic Esophagitis
- Occupational exposure and asthma
- Nitric Oxide and Endothelin Effects
- Pediatric health and respiratory diseases
- Mesenchymal stem cell research
- Immune Response and Inflammation
- Fibroblast Growth Factor Research
- Neuroscience of respiration and sleep
- Contact Dermatitis and Allergies
- Indoor Air Quality and Microbial Exposure
- Congenital Diaphragmatic Hernia Studies
- Pulmonary Hypertension Research and Treatments
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Dermatology and Skin Diseases
- Airway Management and Intubation Techniques
- Food Allergy and Anaphylaxis Research
IRCCS Istituto Auxologico Italiano
1986-2015
Università Cattolica del Sacro Cuore
1985-2015
Biomedical Research Institute
2003-2015
Freiwillige Akademische Gesellschaft
2004
Czech Academy of Sciences, Institute of Experimental Medicine
1993-1998
Università Campus Bio-Medico
1995
St. Joseph’s Healthcare Hamilton
1995
McMaster University
1989-1995
St. Joseph's Hospital
1995
John Hunter Hospital
1995
The mechanisms contributing to airway wall remodeling in asthma are under investigation identify appropriate therapeutic targets. Bronchial myofibroblasts would represent an important target because they play a crucial role the genesis of subepithelial fibrosis, characteristic feature process, but their origin is poorly understood. We hypothesized that originate from fibrocytes, circulating cells with unique expressing hemopoietic stem cell Ag CD34 and collagen I. In this study we show...
Abstract The respiratory epithelium represents the first barrier encountered by airborne Ags. Two major dust mite Ags, Der p3 and p9, are serine proteases that may activate lung epithelial cells interaction with protease-activated receptor 2 (PAR-2). In this study both p9 cleaved peptide corresponding to N terminus of PAR-2 at activation site. Both Ags sequentially stimulated phosphoinositide hydrolysis, transient cytosolic Ca2+ mobilization, release GM-CSF eotaxin in human pulmonary cells....
The reproducibility of sputum cell counts was examined and the in patients with asthma were compared those chronic bronchitis. Three groups subjects studied. Sputum from eight production studied to determine counts. findings 10 non-smokers uncomplicated by other airway disease at time an exacerbation (group 2) smokers cough but no features 3). plugs selected microscopy ensure their origin lower respiratory tract. A total count performed on a trypsinised suspension, differential metachromatic...
SUMMARY We investigated the kinetics of allergen-induced eotaxin expression and its relationship to eosinophil accumulation activation in airways patients with allergic asthma. Twenty-four asthma late asthmatic responses allergen inhalation were randomly allocated into three groups eight each, who received bronchoscopy bronchial biopsies BAL at 2, 4 24 h, respectively, after diluent allergen. The mRNA protein release evaluated by situ hybridization, immunohistochemistry, immunocytochemistry,...
Endothelin, synthesized by endothelial cells, is the most potent vasoconstrictor and bronchoconstrictor agent known. We investigated endothelin release from human bronchial epithelial cells binding of peptide to autologous smooth muscle in culture. Epithelial were isolated enzymatic digestion tissue obtained on surgery, cultured confluency standard methods. stained positively for cytokeratin filaments. Smooth uniformly alpha-smooth actin. Immunoreactive contents supernatants extracted C8...
We have previously demonstrated that human bronchial smooth muscle cells possess specific binding sites for the potent bronchoconstrictive peptide endothelin 1 and primary cultures of epithelial constitutively produce an endothelin-like material binds to cell receptors with a kinetic ability analogous shown by authentic peptide. To evaluate potential role airway epithelium-derived in pathogenesis asthma, we examined here expression 6 patients symptomatic asthma reversible airflow...
Cultured human bronchial epithelial cells constitutively produce granulocyte/macrophage colony-stimulating factor (GM-CSF). An upregulation of the synthesis and release GM-CSF from those might contribute to persistence infiltration local activation inflammatory in some diseases airways, such as asthma. Increased levels immunoreactive biologically active interleukin-1 (IL-1) have been identified airway secretions asthmatic patients, together with an increase contents. As IL-1 is known...
The fibrocytes are thought to serve as a source of newly deposited collagens I and III during reparative processes in certain fibrotic disorders, but their matrix remodelling properties incompletely understood. We evaluated ability produce several extracellular (ECM) components, comparison with fibroblasts, participate collagen turnover. gene expression profile differed from that fibroblasts because constitutively expressed relatively high levels the mRNA encoding VI significantly lower I,...
We have examined the in vitro interaction between bronchial epithelial cells and eosinophils derived from five asthmatics by determining effect of cell-conditioned medium on survival activation peripheral blood eosinophils. The supernatants six normal donors were used as control. asthmatic significantly increased cultured for 3 (P less than 0.025) 6 0.05) days. incubation with 1 h also generation superoxide anion release leukotriene C4, triggered phorbol myristate acetate calcium ionophore,...