A5043407970- Spectroscopy Techniques in Biomedical and Chemical Research
- Spectroscopy and Chemometric Analyses
- Metabolomics and Mass Spectrometry Studies
- Microfluidic and Bio-sensing Technologies
- Protein Interaction Studies and Fluorescence Analysis
- Mass Spectrometry Techniques and Applications
- Bee Products Chemical Analysis
Technological University Dublin
2020
22q11 Ireland
2018-2019
Analysis of bodily fluids using vibrational spectroscopy has attracted increasing attention in recent years. In particular, infrared spectroscopic screening blood products, particularly serum, for disease diagnostics been advanced considerably, attracting commercial interests. However, analyses requiring quantification endogenous constituents or exogenous agents are less well advanced. Recent advances towards this end reviewed, focussing on and Raman human serum. The importance analysis the...
This study explores the suitability of Raman spectroscopy as a bioanalytical tool, when coupled with ultra-filtration and multivariate analysis, to detect imbalances in both high molecular weight (total protein content, γ globulins albumin) low (urea glucose) fractions same samples human patient serum, native liquid form. Ultracentrifugation was employed separate concentrate serum. Initially, aqueous solutions respective species, covering physiologically relevant concentration ranges, were...
Analysis of biomarkers present in the blood stream can potentially deliver crucial information on patient health and indicate presence numerous pathologies. The potential vibrational spectroscopic analysis human serum for diagnostic purposes has been widely investigated and, recent times, infrared absorption spectroscopy, coupled with ultra-filtration multivariate techniques, attracted increasing attention, both clinical commercial. However, such methods commonly employ a drying step, which...
This study explores the potential of Raman spectroscopy, coupled with multivariate regression techniques and a protein separation technique (ion exchange chromatography), to quantitatively monitor diagnostically relevant changes in high molecular weight proteins liquid plasma. Measurement protocols detect imbalances plasma as an indicator various diseases using spectroscopy are optimised, such that strategic clinical applications for early stage disease diagnostics can be evaluated. In...
A methodology is proposed, based on Raman spectroscopy coupled with multivariate analysis, to determine the Limit of Detection (LOD) and Quantification (LOQ) for therapeutic drug monitoring in human serum, using examples Busulfan, a cell cycle non-specific alkylating antineoplastic agent, and, Methotrexate, chemotherapeutic agent immune system suppressant. In this study, ultrafiltration employed fractionate spiked pooled serum efficiently recover filtrate prior performing analysis. The...