A5088646150- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- CAR-T cell therapy research
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Metabolism, Diabetes, and Cancer
- RNA and protein synthesis mechanisms
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Cell Image Analysis Techniques
- Luminescence and Fluorescent Materials
- RNA Interference and Gene Delivery
- Luminescence Properties of Advanced Materials
- bioluminescence and chemiluminescence research
- Medical Research and Treatments
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- Immune Cell Function and Interaction
- Radiation Detection and Scintillator Technologies
University of Minnesota
2018-2025
Calvin University
2016
Busan Institute of Science and Technology
2015
Abstract Recombinant adeno‐associated virus (rAAV) is among the most commonly used vectors for gene therapy. It produced by transfection of HEK293 cells with three plasmids each containing vector genome including interest (GOI), helper functions, and rep cap genes replication capsid formation. To meet potential clinical needs, productivity production system needs to be enhanced. A better process characterization will further advance our insights into ways enhance productivity. Here, we...
Influenza A virus (IAV) is a respiratory pathogen that has caused significant mortality throughout history and remains global threat to human health. Although much known about IAV replication, the regulation of replication dynamics not completely understood.
Recombinant adeno-associated viruses (rAAV) are important gene delivery vehicles for therapy applications. Their production relies on plasmid transfection or virus infection of producer cells, which pose a challenge in process scale-up. Here, we describe template transfection-free, helper virus-free rAAV cell line using synthetic biology approach. Three modules were integrated into HEK293 cells including an genome and multiple inducible promoters controlling the expression AAV Rep, Cap,...
Abstract Recombinant adeno‐associated virus (rAAV) is among the most commonly used in vivo gene delivery vehicles and has seen a number of successes clinical application. Current manufacturing processes rAAV employ multiple plasmid transfection or rely on infection face challenges scale‐up. A synthetic biology approach was taken to generate stable cell lines with integrated genetic modules, which produced upon induction albeit at low productivity. To identify potential factors that...
Abstract The augmentation of transgene copy numbers is a prevalent approach presumed to enhance transcriptional activity and product yield. CHO cell lines engineered via targeted integration (TI) offer an advantageous platform for investigating the interplay between gene number, mRNA abundance, yield, quality. Our investigation revealed that incrementally elevating both IgG heavy chain (HC) light (LC) concurrently resulted in attainment plateaus levels titers, notably occurring beyond four...
For the biomanufacturing of protein biologics, establishing stable cell lines with high transgene transcription is critical for productivity. Modern genome engineering tools can direct insertion to a specified genomic locus and potentially become valuable tool line generation. In this study, authors survey integration sites their transcriptional activity identify characteristics desirable regions. A lentivirus containing destabilized Green Fluorescent Protein (dGFP) used infect Chinese...
Natural killer (NK) cells are in development for allogeneic immunotherapy; however, such use as off-the-shelf medicines, NK need to undergo ex vivo expansion, typically through activation with feeder and cytokines, generate sufficient clinical applications. Upon stimulation the presence of profound changes gene expression, altering their metabolic activity, cell cycle progression, growth behavior, but precise that drive this transformation remain poorly understood. In study, we identified...
An important quality attribute of a recombinant adeno-associated virus (rAAV) as therapeutic vector is its infectivity. Current assays to quantify infectious rAAV rely on coinfection with helper such adenovirus (Ad), which requires preparation and introduces additional variability. A simple method that has high sensitivity removes the need for would improve assay consistency facilitate high-throughput applications producer cell line development. In this study, we describe stable was...
Abstract The facilitated glucose transporter GLUT1 (SLC2A1) is an important mediator of homeostasis in humans. Though it found most cell types to some extent, the level expression across different can vary dramatically. Prior studies erythrocytes—which express particularly high levels GLUT1—have suggested that able form tetrameric complexes with enhanced transport activity. Whether dynamic aggregation also occurs more modest GLUT1, however, unclear. To address this question, we developed a...
Different regions of a mammalian genome have different accessibilities to transcriptional machinery. The integration site transgene affects how actively it is transcribed. Highly accessible genomic called super-enhancers been recently described as strong regulatory elements that shape cell identity. Super-enhancers identified in Chinese hamster ovary (CHO) cells using the Assay for Transposase-Accessible Chromatin Sequencing (ATAC-seq). Genes near super-enhancer had high transcript levels...