Recently, long noncoding RNAs (lncRNA) have emerged as new gene regulators and prognostic markers in several cancers, including renal cell carcinoma (RCC). In this study, we investigated the contributions of lncRNA MALAT1 RCC with a specific focus on its transcriptional regulation interactions Ezh2 miR-205. We found that expression was higher human tissues, where it associated reduced patient survival. silencing decreased proliferation invasion increased apoptosis. Mechanistic investigations...

Abstract BACKGROUND: MicroRNAs (miRNAs) are small noncoding RNAs that regulate the expression of approximately 60% all human genes. They play important roles in numerous cellular processes, including development, proliferation, and apoptosis. Currently, it is believed miRNAs elicit their effect by silencing target In this study, authors demonstrated miRNA‐205 (miR‐205) induced interleukin (IL) tumor suppressor genes IL24 IL32 targeting specific sites promoters. METHODS: The methods used...

Objective: Two tripeptides (Val-Pro-Pro and Ile-Pro-Pro) that have inhibitory activities for angiotensin I-converting enzyme are produced in milk fermented with Lactobacillus helveticus. In this study we evaluated the effect safety of powdered L. helveticus CM4 on subjects high-normal blood pressure or mild hypertension.Methods: A randomized, placebo-controlled, double-blind was conducted using 40 (HN group) hypertension (MH group). Each subject ingested 6 test tablets (12 g) containing...

The above article, published online on 22 April 2008 in Wiley Online Library ( wileyonlinelibrary.com ), has been retracted by agreement between the authors, journal Editor Chief, Prof. Peter Lichter, and John & Sons Ltd. retraction agreed due to errors identified Figs, 3, 4, 5. concerns about these figures cannot be resolved because original data can no longer retrieved. Reference Kikuno N , Shiina H Urakami S Kawamoto K Hirata Tanaka Y Majid Igawa M Dahiya R. . Int J Cancer 123 : 552 –...

BTG3 / ANA APRO4 has been reported to be a tumor suppressor gene in some malignancies. It constitutes important negative regulatory mechanism for Src-mediated signaling, regulator of the cell cycle and inhibits transcription factor E2F1. We report that is downregulated renal cancer inactivation through promoter hypermethylation. Quantitative real-time polymerase chain reaction (PCR) showed was tissues cells. Genistein 5-aza-2′-deoxycytidine (5Aza-C) induced messenger RNA (mRNA) expression...

MiR-145 is downregulated in various cancers including prostate cancer. However, the underlying mechanisms of miR-145 downregulation are not fully understood. Here, we reported that was silenced through DNA hypermethylation and p53 mutation status laser capture microdissected (LCM) cancer matched adjacent normal tissues. In 22 27 (81%) tissues, significantly compared with Further studies on mechanism showed methylated at promoter region both tissues 50 different types cell lines. seven lines...

Abstract Genistein (4′,5,7-trihydroxyisoflavone) is the most abundant isoflavone found in soybean. The effects of genistein on various cancer cell lines have been extensively studied but precise molecular mechanisms are not known. We report here epigenetic mechanism action androgen-sensitive (LNCaP) and androgen-insensitive (DuPro) human prostate lines. induced expression tumor suppressor genes p21 (WAF1/CIP1/KIP1) p16 (INK4a) with a concomitant decrease cyclins. There was G0-G1 cycle arrest...

Abstract BACKGROUND: B‐cell translocation gene 3 ( BTG3 / ANA APRO4 ) is a candidate tumor suppressor in some malignancies. We report here that transcriptionally down‐regulated prostate cancer and the mechanism of inactivation through promoter hypermethylation. METHODS: Prostate normal cell lines were treated with different doses genistein 5‐aza‐2′‐deoxycytidine (5Aza‐C). messenger ribonucleic acid (mRNA) expression was determined by quantitative real‐time polymerase chain reaction tissues...

Abstract Purpose: Aberrant promoter hypermethylation of Wnt-antagonist genes contributes to the pathogenesis several cancers. We hypothesized that combined methylation analysis could improve their use as a panel biomarkers for diagnosing and staging bladder Experimental Design: Samples (54 total) tumor corresponding normal mucosa were analyzed expression levels six (sFRP-1, sFRP-2, sFRP-4, sFRP-5, Wif-1, Dkk-3). To increase sensitivity/specificity detection, score (M score), new method...

MicroRNA-34a (miR-34a), a potent mediator of tumor suppressor p53, has been reported to function as and miR-34a was found be downregulated in prostate cancer tissues. We studied the functional effects on c-Myc transcriptional complexes PC-3 cells. Transfection into cells strongly inhibited vitro cell proliferation, invasion promoted apoptosis. also significantly vivo xenograft growth nude mice. expression oncogene by targeting its 3' UTR shown luciferase reporter assays. repress RhoA,...

Recently miR-182 has been reported to be over-expressed in prostate cancer (PC) tissues, however detailed functional analysis of miR-182-5p not carried out. The purpose this study was to: 1. analyze the function cancer, 2. assess its usefulness as a tumor marker, 3. identify target genes PC, 4. investigate potential for inhibitor used PC treatment. Initially we found that expression significantly higher tissues and cell lines compared normal cells. Moreover high associated with shorter...

The minichromosome maintenance (MCM) gene family is essential for DNA replication and frequently upregulated in various cancers. Here, we examined the role of MCM2 prostate cancer effect microRNA-1296 (miR-1296), genistein, trichostatin A (TSA) on MCM complex. Profiling results showed that expression genes was higher tumor samples. Genistein TSA significantly downregulated all genes. Genistein, TSA, small interfering RNA duplexes caused a significant decrease S phase cell cycle. There also...

Abstract Cancer pathogenesis is restricted by stresses that compromise cell division and survival. In this study, we identify miR-708, a little studied member of set microRNAs have been implicated in stress control, as an important tumor suppressor renal carcinoma (RCC). miR-708 expression was attenuated widely human RCC specimens. Restoration lines decreased growth, clonability, invasion, migration elicited dramatic increase apoptosis. Moreover, intratumoral delivery sufficient to trigger...

Background MicroRNA-21 is up-regulated in a variety of cancers like, breast, colorectal, lung, head and neck etc. However, the regulation miR-21 renal cell carcinoma (RCC) has not yet been studied systematically. Methods Results We measured levels 54 pairs kidney their normal matched tissues by real-time PCR. The expression level was correlated with 5 year survival pathological stage. Functional studies were done after inhibiting RCC lines. vitro vivo effects chemo preventive agent genistein...

Wnt-signalling has an important role in renal cancer and it is modulated by genistein other cancers. Recently, microRNAs (miRNAs) have emerged as new regulators of gene expression. Thus, we focused on miRNAs to examine the regulatory mechanism pathway cell carcinoma (RCC). Initially, investigated effect (TOPflash reporter assay (TCF assays)) cells, using microarray identified candidate whose expression was decreased genistein. We performed functional analyses relationship between miRNA...

Abstract The Src family of protein kinases (SFK) plays key roles in regulating fundamental cellular processes, including cell growth, differentiation, shape, migration, and survival, specialized signals various malignancies. pleiotropic functions SFKs cancer make them promising targets for intervention. Here, we sought to investigate the role microRNA-205 (miR-205) inhibition Src-mediated oncogenic pathways renal cancer. We report that expression miR-205 was significantly suppressed lines...

Recently several microRNAs (miRNAs) have been found to be regulated by genistein in cancer cells. In this study, we focused on the gene regulatory effect of microRNA and its target genes prostate (PC). Initially, investigated cells identified that expression miRNA-1260b was decreased genistein. We performed functional analyses relationship between patient outcomes. Two (sFRP1 Smad4) miR-1260b were based computer algorithm 3′UTR luciferase assay carried out determine direct miRNA regulation...

Background. Several animal models for xenogenic (xeno) graft versus host disease (GVHD) have been developed in immunodeficient mice, such as C.B-17-scid and nonobese diabetes (NOD)/severe combined immunodeficiency (SCID), by human peripheral blood mononuclear cell (hPBMC) transplantation. However, these pose problems because they require sublethal total body irradiation of the mice a large number hPBMCs to induce GVHD, timing onset GVHD is also unstable. The aim this study establish improved...

Background miR-23b is located on chromosome number 9 and plays different roles in organs especially with regards to cancer development. However, the functional significance of miR-23b-3p renal cell carcinoma (RCC) has not been reported. Methods Results We measured levels 29 pairs their normal matched tissues using real-time PCR. The expression level was correlated 5 year survival rate patients. In 15 cases (52%), found be high. All patients moderate low survived years, while those high...

Abstract The lead(II) ion-selective ceramic membrane electrode developed by sintering a mixture of lead, silver, and cuprous sulfides showed sensitivity, selectivity, other response characteristics well suited to analytical utilization. Nernstian slope was obtained over concentration range from 10−1 10−6m Pb2+ in activity, the had 10−1–10−7m when contained less than 30 wt% sulfide more 1 lead sulfide. Among common ions, cupric, mercury(II), ferric, iodide ions interfered seriously. About 10...

The purpose of this study was to identify prostate cancer (PC) oncogenic microRNAs (miRs) based on miR microarray and investigate whether these miRs may be useful as PC biomarkers. Initially, we carried out real-time PCR using RWPE-1, PC-3, DU-145 LNCaP cells. To the function miR-183, used a miR-183 knockdown inhibitor in cell growth wound-healing assays. We several algorithms confirmed that they are directly regulated by miR-183. identified three potential (miR-146a, miR-767-5P). expression...

Onco-miR-182-5p has been reported to be over-expressed in bladder cancer (BC) tissues however a detailed functional analysis of miR-182-5p not carried out BC. Therefore the purpose this study was to: 1. conduct cancer, 2. assess its usefulness as tumor marker, 3. identify target genes Initially we found that expression significantly higher compared normal and high associated with shorter overall survival BC patients. To significance miR-182-5p, precursor observed cell proliferation,...