A5052950098- Redox biology and oxidative stress
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Chromosomal and Genetic Variations
- Cancer Cells and Metastasis
- HIV Research and Treatment
- RNA modifications and cancer
- Microtubule and mitosis dynamics
- Cellular transport and secretion
- PI3K/AKT/mTOR signaling in cancer
- Biochemical and Molecular Research
- Genomics and Chromatin Dynamics
- Mass Spectrometry Techniques and Applications
- Autophagy in Disease and Therapy
- RNA and protein synthesis mechanisms
- Lung Cancer Treatments and Mutations
- Prostate Cancer Treatment and Research
- interferon and immune responses
- Cutaneous lymphoproliferative disorders research
- FOXO transcription factor regulation
- Heat shock proteins research
- Cancer, Hypoxia, and Metabolism
- Cancer, Stress, Anesthesia, and Immune Response
- Plant Virus Research Studies
- thermodynamics and calorimetric analyses
Inserm
1991-2024
Institut Franco-Allemand de Recherches de Saint-Louis
2024
Institut Cochin
2005-2022
Université Paris Cité
2005-2022
Centre National de la Recherche Scientifique
2001-2022
École Normale Supérieure Paris-Saclay
2013-2017
La Ligue Contre le Cancer
2002
Cancer Genetics (United States)
2002
Scripps Research Institute
2002
Institut Gustave Roussy
1991-2001
Single base pair mutations that alter the function of tumor suppressor genes and oncogenes occur frequently during oncogenesis. The guardian genome, p53, is inactivated by point mutation in more than 50% human cancers. Synthetic small inhibiting RNAs (siRNAs) can suppress gene expression mammalian cells, although their degree selectivity might be compromised an amplification mechanism. Here, we demonstrate a single difference siRNAs discriminates between mutant WT p53 cells expressing both...
LINEs are endogenous mobile genetic elements which have dispersed and accumulated in the genomes of most higher eukaryotes via germline transposition, with up to 100 000 copies for human LINE-1 (L1H) sequences. Although severely repressed normal tissues, L1H is still functional, evidence both somatic—essentially tumors—transpositions. Yet, no transcription factor that could regulate their be responsible transposition has hitherto been described. Here we show factors belonging family...
TNF-α is a potent proinflammatory cytokine that regulates immune and inflammatory responses programmed cell death. stimulation causes nuclear translocation of several NF-κB dimers, including RelA/p50 RelB/p50. However, contrary to RelA, RelB entering the nucleus in response cannot bind DNA mouse embryonic fibroblasts, strongly suggesting DNA-binding activity modulated by additional mechanisms. Here, we demonstrate promotes association RelA with TNF-α-induced RelA/RelB heterodimers do not κB...
Using an assay for retrotransposition detection (T. Heidmann, O. and J. F. Nicolas, Proc. Natl. Acad. Sci. USA 85:2219-2223, 1988), we demonstrated that a defective retrovirus deleted the gag, pol, env open reading frames can disperse in genome of human HeLa cells by intracellular transposition, at frequency close to 10(-6) events per cell generation. Transposition requires cooperation trans gag pol gene products may be associated with release low amounts noninfectious retroviruslike...
The screening of two different retroviral cDNA expression libraries to select genes that confer constitutive doxorubicin resistance has in both cases resulted the isolation heat shock factor 1 (HSF1) transcription factor. We show HSF1 induces a multidrug phenotype occurs absence or cellular stress and is mediated at least part through activation gene (MDR-1). This drug does not correlate with an increased shock-responsive (heat protein genes, HSPs). In addition, mutants lacking HSP are also...
Chromatin assembly factor 1 (CAF-1) is a protein complex formed of three subunits, p150, p60, and p48, conserved from the yeast Saccharomyces cerevisiae to humans, which can promote nucleosome onto newly replicated DNA.In S. cerevisiae, deletion genes encoding any CAF-1 subunits (cac⌬ mutants), although nonlethal, results in silencing defect packaged into heterochromatin.Here we report on mammalian cell model that devised monitor gene its reversal quantitative manner.This relies use line...
Metastasis involves the dissemination of single or small clumps cancer cells through blood lymphatic vessels and their extravasation into distant organs. Despite strong regulation metastases development by a cell dormancy phenomenon, dormant state remains poorly characterized due to difficulty in vivo studies. We have recently shown vitro that clonogenicity prostate is regulated phenomenon strongly induced when are cultured both at low density slightly hypertonic medium. Here, we RT-qPCR...
Cellular quiescence is a reversible cell growth arrest that often assumed to require persistence of non-permissive external conditions for its maintenance. In this work, we showed androgen could induce quiescent state self-sustained in cell-autonomous manner through "hit and run" mechanism receptor-expressing prostate cancer cells. This phenomenon required the set-up sustained redox imbalance TGFβ/BMP signaling were dependent on culturing cells at low density. At medium density, androgens...
We devised an indicator gene for retrotransposition, nlsLacZRT, which contains the Escherichia coli lacZ fused to a nuclear location signal (nlsLacZ), engineered in such way that is expressed only if structure it has been inserted transposes itself through RNA intermediate. A cloned murine leukemia retrovirus with ecotropic host range (Moloney virus), rendered defective by large deletion encompassing three viral gag, pol, and env open reading frames, was marked this introduced transfection...
Retroviral particles contain a dimer of two genomic RNA molecules, linked by noncovalent intermolecular bonds. Studies electron microscopy viral extracted from virions as well in vitro studies have implicated sequence, designated the linkage sequence (DLS), dimerization process. The DLS has been localized within short region encompassing psi packaging between nucleotides 212 and 563 for Moloney murine leukemia retrovirus (MoMLV) RNA. In this report, we show that RNAs lacking both...
Self-sustained quiescence (SSQ) has been characterized as a stable but reversible non-proliferative cellular state that limits the cloning of cultured cancer cells. By developing refined clonogenic assays, we showed here cells in SSQ can be selected with anticancer agents and culture at low cell density induced pancreas prostate adenocarcinoma Pre-culture 3D or their pretreatment pharmacological inhibitors mechanistic target rapamycin (mTOR) synergize for induction Beclin-1-dependent manner....