Improvements in poultry production within the past 50 years have led to increased muscle yield and growth rate, which may be contributing an rate development of new disorders chickens. Previously reported conditions are generally associated with poor meat quality traits a significant negative economic impact on industry. Recently, novel myopathy phenotype has emerged is characterized by palpably "hard" or tough breast muscle. The objective this study identify underlying biological mechanisms...

Wooden Breast Disease (WBD), a myopathy that frequently affects modern broiler chickens, is disorder has been associated with significant economic losses in the poultry industry. To examine tissue changes onset and early pathogenesis of this disorder, time-series experiment was conducted using chickens from high-breast-muscle-yield, purebred commercial line. Birds were raised for up to seven weeks, subset birds sampled weekly. muscle tissues extracted at necropsy processed analysis by light...

Wooden Breast Disease (WBD), a myopathy in commercial broiler chickens characterized by abnormally firm consistency of the pectoral muscle, impacts poultry industry negatively due to severe reduction meat quality traits. To unravel molecular profile associated with onset and early development WBD chickens, we compared time-series gene expression profiles Pectoralis (P.) major muscles between unaffected affected birds from high-breast-muscle-yield, purebred line. P. biopsy samples were...

Abstract This integrative study of transcriptomics and metabolomics aimed to improve our understanding Wooden Breast myopathy (WB). muscle samples from 8 WB affected unaffected male broiler chickens 47 days age were harvested for metabolite profiling. Among these 16 samples, 5 6 also underwent gene expression The Joint Pathway Analysis was applied on 119 metabolites 3444 genes exhibiting differential abundance or between chickens. Mitochondrial dysfunctions in suggested by higher levels...

This study aims to use spatial transcriptomics characterize the cell-type-specific expression profile associated with microscopic features observed in Wooden Breast myopathy. 1 cm3 muscle sample was dissected from cranial part of right pectoralis major three randomly sampled broiler chickens at 23 days post-hatch and processed Visium Spatial Gene Expression kits (10X Genomics), followed by high-resolution imaging sequencing on Illumina Nextseq 2000 system. WB classification based...

U ndoubtedly, the veterinary medical field attracts individuals with high levels of compassion and empathy drive to care for others.With increased public recognition human-animal bond greater value placed on it, veterinarians their staff members are increasingly expected display consideration in workplace.Individuals tend enter remain profession part because satisfaction (the joy or sense achievement found helping others providing high-quality patient care), 1,2 overall, other animal...

A limited outbreak of nephropathogenic infectious bronchitis (NIB) occurred in three Delmarva (DMV) commercial broiler chicken flocks 2011. Isolates NIB virus (NIBV)—DMV/1639/11, DMV/3432/11, and DMV/3902/11—were characterized by sequence analysis the N-terminal subunit (S1) spike (S) gene. Findings indicated that isolates were identical to each other PA/9579A/10, a 2010 isolate from poultry Pennsylvania. The 2011 appear have originated 1997–2000 DMV/1639/11 PA/9579A/10 determined be...

The efficacy of commercially available recombinant herpesvirus turkeys-infectious bursal disease (rHVT-IBD) virus vaccines was studied in broiler chickens derived from an IBDV-vaccinated breeder flock at 30 wk age (Trial 1) and 60 2). In parallel, specific-pathogen-free (SPF) white leghorn were used to evaluate vaccine control for the effects maternally antibodies (MDA) associated with chickens. Broilers SPF leghorns vaccinated subcutaneously neck 1 day Vaxxitek® HVT IBD or Vectormune®...

This study evaluates the impact of light-emitting diode (LED), cold cathode fluorescent (CCFL), and incandescent lamps on broiler performance. Male Ross 708 broilers (n=672) were raised to 6 wk age in 8 black-out modified large colony houses, under identical intermittent lighting conditions using 4 unique types lamps, which gradually dimmed throughout study. Incandescent served as control; experimental technologies tested included CCFL 2 different LED lamps. Each technology was duplicate for...

Wooden breast syndrome is a widespread and economically important myopathy vasculopathy of fast growing, commercial broiler chickens, primarily affecting birds with high feed efficiency large muscle yield. To investigate potential systemic physiological differences between affected unaffected by wooden breast, total 103 market-age Cobb 500 broilers were sampled for 13 blood parameters the relative weights pectoralis major muscle, minor external oblique wing, heart, lungs, liver, spleen....

Layer hens (egg-laying chickens) and broiler breeders (breeding stock for meat-producing are crucial to the world's food supply as a reliable source of protein. They also an emerging animal model study human reproductive disease. As field poultry research develops, health function layer hen breeder ovary will be important point both agricultural biomedical researchers. One challenges presented by this interest is need replicable techniques that all researchers can employ in ovarian specimen...

Current diagnostic methods for wooden breast and white striping, common muscle myopathies of modern commercial broiler chickens, rely on subjective examinations the pectoralis major muscle, time-consuming microscopy, or expensive imaging technologies. Further research these disorders would benefit from more quantitative objective measures disease severity that can be used in live birds. To this end, we utilized untargeted metabolomics alongside two statistical approaches to evaluate plasma...

Zika virus (ZIKV) is a mosquito-borne flavivirus associated with microcephaly and other neurological disorders in infants born to infected mothers. Despite being declared an international emergency by the World Health Organization, very little known about mechanisms of ZIKV pathogenesis or long-term consequences maternal infection affected offspring, largely due lack appropriate rodent models. To address this issue, our lab has developed working model prenatal rats. In study, we immune...

This study evaluates the impact of light-emitting diodes (LEDs), cold cathode fluorescent lamps (CCFLs), and incandescent on broiler welfare in response to recent interest agriculture industry transition more energy-efficient lighting technologies. Male Ross 708 broilers (n = 672) were raised 6 wk age 8 light-tight modified large colony houses under identical intermittent conditions using 4 unique types lamps, which gradually dimmed throughout study. Incandescent served as control;...

Clinical information supplied to diagnostic laboratories through biopsy submission forms is crucial accurate, timely diagnosis and clinicopathologic correlation between microscopic findings the clinical condition of patient. The current study attempts quantify prevalence deficient inadequate submissions in veterinary service determine whether form characteristics, such as open or closed nature presence specific prompts, influence reporting essential case information. hypotheses this are,...

Rupture of the gastrocnemius tendon is a costly challenge for poultry industry due to bird culling from clinical lameness and leg condemnations at processing plant. In this case, broiler chicken producer experienced dramatic increase in processing, coinciding with temporary age weight (from 62 days 10.25 pounds or 4.66 kg 67 11 5 body weight). Gross lesions condemned limbs included acute rupture corresponding hemorrhage edema. Histologic analysis, however, revealed that "tendon rupture"...

Objectives: This study evaluated the protective effect of apiaceous vegetable (API) consumption (celery and parsnip) on acrolein (ACR) exacerbated lung pathologies in Apolipoprotein E knockout (ApoE–/–) mice utilizing RNA sequencing analysis to identify differentially expressed genes (DEGs) which were subjected bioinformatics prediction-based software, Ingenuity Pathway Analysis (IPA), predict activation or inhibition canonical pathways between groups. Methods: Thirty-three ApoE–/– randomly...

Wooden Breast Disease (WBD) is a novel muscle disorder in the poultry industry observed to frequently affect breast muscles of high-yielding modern broilers. Characterized by extreme stiffening on palpation pectoral region, WBD known result significant economic loss and may potentially cause behavioral alterations reduced welfare birds. To examine tissue changes associated with onset pathogenesis this disorder, time-series experiment was conducted using chickens from...

Abstract This study aims to use spatial transcriptomics characterize the cell-type-specific expression profile associated with microscopic features observed in Wooden Breast myopathy. 1 cm 3 muscle sample was dissected from cranial part of right pectoralis major three randomly sampled broiler chickens at 23 days post-hatch and processed Visium Spatial Gene Expression kits (10X Genomics), followed by high-resolution imaging sequencing on Illumina Nextseq 2000 system. WB classification based...