A5052677683- Lipoproteins and Cardiovascular Health
- Cancer, Lipids, and Metabolism
- Cholesterol and Lipid Metabolism
- SARS-CoV-2 and COVID-19 Research
- Cassava research and cyanide
- COVID-19 Clinical Research Studies
- Banana Cultivation and Research
- Atherosclerosis and Cardiovascular Diseases
- Pharmacogenetics and Drug Metabolism
- Drug-Induced Hepatotoxicity and Protection
- vaccines and immunoinformatics approaches
- Drug Transport and Resistance Mechanisms
- Inflammatory mediators and NSAID effects
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Plant Micronutrient Interactions and Effects
- Phytase and its Applications
- Antioxidant Activity and Oxidative Stress
- Agricultural and Food Sciences
- Natural Antidiabetic Agents Studies
- RNA Interference and Gene Delivery
- Immune cells in cancer
- Vaccine Coverage and Hesitancy
- Antiplatelet Therapy and Cardiovascular Diseases
- Food Allergy and Anaphylaxis Research
- Ethics and Legal Issues in Pediatric Healthcare
Universidade de São Paulo
2016-2025
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2021-2023
Instituto do Coração
2021
University of Regensburg
2010
University Hospital Regensburg
2010
Friedrich-Alexander-Universität Erlangen-Nürnberg
1992-1995
Klinik und Poliklinik für Psychosomatik und Psychotherapie
1988
Zentrallaboratorium Deutscher Apotheker
1981
Abstract We tested apolipoprotein E phenotypes in 1557 normolipidemic factory workers and 822 hyperlipidemic hospital patients. distinguished six different determined their frequencies normolipidemia (factory workers), hypertriglyceridemia, hypercholesterolemia, mixed hyperlipidemia. For the three homozygous E3/3, E4/4, E2/2, percentage distribution group was 62.2%, 2.2%, 0.9%, respectively; for heterozygous E4/3, E3/2, E4/2, we of 19.9%, 11.7%, 2.9%, respectively. A higher prevalence E2/2...
Patients with Inborn Errors of Immunity (IEI) are at higher risk severe SARS-CoV-2 infection. We evaluated humoral and cellular responses to COVID-19 vaccines in Brazilian patients IEI healthy controls. Fifty-five (13-61 years) 60 controls (13-71 received inactivated (CoronaVac), non-replicating virus-vectored (ChAdOx1 nCoV-19, AstraZeneca) or monovalent mRNA (Original strain BNT162b2, Pfizer-BioNTech) bivalent (Original/Omicron BA.1, were sampled five times. Diagnoses included common...
Abstract We investigated the anti-SARS-CoV-2 post-vaccine response through serum and salivary antibodies, antibody neutralizing activity cellular immune in samples from health care workers who were immunized with two doses of an inactivated virus-based vaccine (CoronaVac) had or did not have COVID-19 previously. IgA IgG antibodies directed at spike protein analysed saliva and/or by ELISA chemiluminescence assays; against reference strain B, Gamma Delta SARS-CoV-2 variants evaluated using a...
Increased levels of low-density lipoproteins are well-established risk factors endothelial dysfunction and the metabolic syndrome. In this study, we evaluated effect native lipoprotein (nLDL) oxidized LDL (oxLDL) on expression genes renin-angiotensin system (angiotensin-converting enzyme, ACE; angiotensin II type 1 receptor, AT1) their receptors (low-density receptor: LDLR; lectin-like oxLDL LOX-1; toll-like receptor 4: TLR4) in primary cultures human umbilical vein cells. ACE AT1...
Introduction Considering the likely need for development of novel effective vaccines adapted to emerging relevant CoV-2 variants, increasing knowledge epitope recognition profile among convalescents and afterwards vaccinated with identification immunodominant regions may provide important information. Methods We used an RBD peptide microarray identify IgG IgA binding in serum 71 COVID-19 18 individuals. Results found a set antibody epitopes, each recognized by more than 30% tested cohort,...
Although cassava is of great importance as a starch source for industrial purposes or food consumption, the evaluation different varieties an under‐investigated topic. Thus, in order to contribute identification starches more suitable use and application industry, this study aimed analyze compare physicochemical properties from ten household use, mixed use. In accomplish goal, analyses were performed into thermal pasting properties, granule size, amylopectin branch‐chain‐length distribution...
Cassava (Manihot esculenta Crantz) is a staple food and an important source of starch, the attributes its tuberous root largely depend on variety. The proteome cassava has been investigated; however, to date, no study focused varieties that reveal molecular basis phenotypical characteristics. Therefore, we aimed compare roots six differed in carbohydrates, carotenoids, resistance diseases, among other attributes. Two-dimensional gels showed 146 differential spots between varieties,...
Abstract Recent SARS-CoV-2 variants pose important concerns due to their higher transmissibility ( 1 ) and escape 2 from previous infections or vaccine-induced neutralizing antibodies (nAb). The receptor binding domain (RBD) of the Spike protein is a major nAb target 3 ), but data on its B cell epitopes are still lacking. Using peptide microarray, we identified an immunodominant epitope (S 415-429 recognized by 68% sera 71 convalescent Brazilians infected with ancestral variant. In contrast...
The ATP-binding cassette transporter A1 (ABCA1) is a membrane-bound protein that abundant in macrophages and essential for the first step of reverse cholesterol transport maintenance homeostasis high-density lipoprotein (HDL)-bound cholesterol. Low serum HDL levels are associated with increased risk cardiovascular disease. Homozygous heterozygous mutations ABCA1 gene may be atherosclerosis. Here we report about two c.5398A>C c.2369G>A deficiency serum.
Therapeutic vaccines based on monocyte‐derived dendritic cells have been shown to be promising strategies and may act as complementary treatments for viral infections, cancers, and, more recently, autoimmune diseases. Alpha‐type‐1‐polarized (aDC1s) induce type‐1 immunity with a high capacity produce interleukin‐12p70 (IL‐12p70). In the clinical use of cell‐based therapeutics, injectable solutions can affect morphology, immunophenotypic profile, viability before delivery their survival after...
Abstract Diclofenac (0.5–2 mM) dose‐ and time‐dependently reduces the viability of isolated hepatocytes. This effect cannot be counteracted by calcium channel blockers diltiazem (0.05–0.1 verapamil (0.05–0.5 mM), calmodulin antagonist calmidazolium (0.01 or Quin 2‐AM (0.1 an intracellular chelating agent. On contrary, even accentuates toxic effects diclofenac. It is concluded from these results, that diclofenac causes cell damage other mechanisms than overload.
In the process of population screening for apo E gene polymorphism with PCR and subsequent restriction analysis, we identified a female who demonstrated heterozygosity an unusual fragment caused by loss CfoI site. Sequence analysis was performed carrier mutant allele C ® T substitution at cDNA position 3817 identified, which Arg136 Cys change. The first-line relatives have been screened this rare mutation products. complete lipoprotein parameters determined in probands family. family, only...