A5010904358- Autophagy in Disease and Therapy
- Crystallization and Solubility Studies
- Spectroscopy Techniques in Biomedical and Chemical Research
- X-ray Diffraction in Crystallography
- Cancer Treatment and Pharmacology
- Advanced Fluorescence Microscopy Techniques
- Synthesis and Biological Evaluation
- Phytochemical Studies and Bioactivities
- Natural product bioactivities and synthesis
- Alzheimer's disease research and treatments
- Lung Cancer Treatments and Mutations
- Catalysis and Hydrodesulfurization Studies
- Seismic Performance and Analysis
- Petroleum Processing and Analysis
- Cellular transport and secretion
- Mitochondrial Function and Pathology
- Endoplasmic Reticulum Stress and Disease
- Smart Agriculture and AI
- Agricultural Engineering and Mechanization
- Cancer therapeutics and mechanisms
- Bone and Dental Protein Studies
- Soil Mechanics and Vehicle Dynamics
- Phytochemistry and Bioactive Compounds
- Near-Field Optical Microscopy
- Neurological diseases and metabolism
Kyushu University
2024-2025
University of Fukui
2014-2025
Niigata University
2019-2024
Hamanomachi Hospital
2021-2022
Tokyo Medical and Dental University
2004-2021
Toho University
2021
Niigata University Medical and Dental Hospital
2021
Toho University Omori Medical Center
2021
The University of Tokyo
1979-2020
Institute for Solid State Physics and Optics
2020
MicroRNAs (miRNAs) are evolutionarily conserved, 18- to 25-nucleotide, non-protein coding transcripts that posttranscriptionally regulate gene expression during development. miRNAs also occur in postmitotic cells, such as neurons the mammalian central nervous system, but their function is less well characterized. We investigated role of midbrain dopaminergic (DNs). identified a miRNA, miR-133b, specifically expressed DNs and deficient tissue from patients with Parkinson's disease. miR-133b...
Abstract Objective High mobility group box chromosomal protein 1 (HMGB‐1), a nuclear DNA binding protein, was recently rediscovered as new proinflammatory cytokine. The purpose of this study to demonstrate HMGB‐1 expression in vivo and identify the role pathogenesis rheumatoid arthritis (RA). Methods concentrations synovial fluid (SF) serum from RA osteoarthritis (OA) patients were measured by immunoblot analysis. protein's specific receptor, receptor for advanced glycation end products...
Three types of ficolins have been identified in humans: L-ficolin, M-ficolin, and H-ficolin. Similar to mannose-binding lectin, L-ficolin H-ficolin are the recognition molecules lectin complement pathway. Another human ficolin, is a nonserum ficolin that expressed leukocytes lung; however, little known about its physiologic roles. In this study, we report characterization M-ficolin terms protein localization activity. was localized secretory granules cytoplasm neutrophils, monocytes, type II...
Extracellular matrix production and degradation by bone cells are critical steps in metabolism. Mutations of the gene encoding MMP-2, an extracellular matrix-degrading enzyme, associated with a human genetic disorder characterized subcutaneous nodules, arthropathy, focal osteolysis. It is not known how loss MMP-2 function results pathology. Here, we show that Mmp2(-/-) mice exhibited opposing phenotypes caused impaired osteocytic canalicular network. showed decreased mineral density limb...
Abstract Background Macroautophagy is an evolutionarily conserved mechanism for bulk intracellular degradation of proteins and organelles. Pathological studies have implicated macroautophagy defects in human neurodegenerative disorders aging including Alzheimer’s disease tauopathies. Neuronal deficiency throughout mouse embryonic development results neurodevelopmental early postnatal mortality. However, the role mature CNS neurons, relationship with neuropathology, remains unclear. Here we...
Abstract Leucine-rich repeat kinase 2 ( LRRK2 ) has been linked to several clinical disorders including Parkinson’s disease (PD), Crohn’s and leprosy. Furthermore in rodents, deficiency or inhibition leads lysosomal pathology kidney lung. Here we provide evidence that functions together with a second PD-associated gene, RAB7L1, within an evolutionarily conserved genetic module diverse cellular contexts. In C. elegans neurons, orthologues of RAB7L1 act coordinately ordered pathway regulate...
We report the reduction of threshold voltage, Vth, twisted nematic liquid crystal devices by doping nanoparticles MgO and SiO2. The results are well explained inserting experimentally determined values elastic constants dielectric anisotropy in formula Vth = π√Keff/ε0Δε, where both these quantities decrease due to existence nanoparticles. approximately as √S, S being order parameter. is also shown
High mobility group protein 1 (HMGB1) has been implicated in diverse cellular functions, including determination of nucleosomal structure and stability binding transcription factors to their cognate DNA sequences (1)(2)(3)(4). HMGB1 is also present a membrane-associated form, termed amphoterin, that mediates neurite outgrowth (5). Amphoterin can interact with macrophage cell surface receptors for advanced glycation end products enhance expression tissue-type plasminogen activator...
We developed a new convertible copper(I) complex using 2-pyridyl-4-methylpyrimidine and diphosphine as ligands. This exhibited mechanical bistability based on the inversion motion of pyrimidine ring, leading to dual luminescence behavior. The dynamics was strongly dependent temperature solvent. Variable-temperature (1)H NMR spectra revealed that two isomers interconverted in solution via ring inversion, frozen below 200 K. characteristic CT absorption emission bands solution. Emission...
Degradation of mitochondria through mitophagy contributes to the maintenance mitochondrial function. In this study, we identified that Atg43, a outer membrane protein, serves as receptor in model organism Schizosaccharomyces pombe promote selective degradation mitochondria. Atg43 contains an Atg8-family-interacting motif essential for mitophagy. Forced recruitment Atg8 restores Atg43-deficient cells, suggesting tethers expanding isolation membranes We found import factors, including...
Abstract Mitophagy plays an important role in the maintenance of mitochondrial homeostasis. PTEN-induced kinase (PINK1), a key regulator mitophagy, is degraded constitutively under steady-state conditions. During it becomes stabilized outer membrane, particularly stress conditions, such as treatment with uncouplers, generation excessive reactive oxygen species, and formation protein aggregates mitochondria. Stabilized PINK1 recruits activates E3 ligases, Parkin ubiquitin ligase (MUL1), to...
Mitochondria undergo fission and fusion, their coordinated balance is crucial for maintaining mitochondrial homeostasis. In yeast, the dynamin-related protein Dnm1 a factor acting from outside mitochondria. We recently reported intermembrane space Atg44/mitofissin/Mdi1/Mco8 as novel factor, but relationship between Atg44 remains elusive. Here, we show that required to complete Dnm1-mediated under homeostatic conditions. Atg44-deficient cells often exhibit enlarged mitochondria with...
Muscle disuse induces atrophy through increased reactive oxygen species (ROS) released from damaged mitochondria. Mitophagy, the autophagic degradation of mitochondria, is associated with ROS production. However, mitophagy activity status during disuse-induced muscle has been a subject debate. Here, we developed new reporter mouse line to examine how affected in skeletal muscles. Mice expressing tandem mCherry-EGFP proteins on mitochondria were then used monitor dynamics activity. The mice...
Differentiated muscle cells contain myofibrils and well-organized organelles, enabling powerful contractions. Muscle cell reorganization occurs in response to various physiological stimuli; however, the mechanisms behind this remodeling remain enigmatic due lack of a genetically trackable system. Previously, we reported that subset larval is remodeled into adult abdominal through an autophagy-dependent mechanism Drosophila . To unveil underlying remodeling, performed comparative time-course...
Differentiated muscle cells contain myofibrils and well-organized organelles, enabling powerful contractions. Muscle cell reorganization occurs in response to various physiological stimuli; however, the mechanisms behind this remodeling remain enigmatic due lack of a genetically trackable system. Previously, we reported that subset larval is remodeled into adult abdominal through an autophagy-dependent mechanism Drosophila . To unveil underlying remodeling, performed comparative time-course...
SUMMARY Mitophagy maintains mitochondrial homeostasis through selective degradation of damaged or excess mitochondria. Recently, we identified mitofissin/Atg44, a intermembrane space-resident fission factor, which directly acts on lipid membranes and drives required for mitophagy in yeast. However, it remains unclear whether mitofissin is sufficient mitophagy-associated other factors act from outside the Here, identify outer membrane-resident mitofissin-like microprotein mitophagy, name...