A5024194012- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Plant Pathogens and Resistance
- Plant Pathogenic Bacteria Studies
- Plant Parasitism and Resistance
- Plant tissue culture and regeneration
- Retinal Development and Disorders
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Yeasts and Rust Fungi Studies
- Plant Reproductive Biology
- Biofuel production and bioconversion
- Plant Molecular Biology Research
- Flowering Plant Growth and Cultivation
- Plant Disease Resistance and Genetics
- CRISPR and Genetic Engineering
- Fungal and yeast genetics research
- Plant and Fungal Interactions Research
- Fermentation and Sensory Analysis
- Anesthesia and Neurotoxicity Research
- Protist diversity and phylogeny
- Neuroinflammation and Neurodegeneration Mechanisms
- Pluripotent Stem Cells Research
- Studies on Chitinases and Chitosanases
- Insect-Plant Interactions and Control
Nanjing Agricultural University
2021-2025
China Agricultural University
2019-2024
Southwest Hospital
2017-2024
Army Medical University
2017-2024
Ministry of Agriculture and Rural Affairs
2024
Northwest A&F University
2013-2022
Summary Canker caused by ascomycetous Valsa species are among the most destructive diseases of woody plants worldwide. These pathogens distinct from other because they only effectively attack tree bark in field. To unravel potential adaptation mechanism colonization, we examined genomes mali and pyri that preferentially infect apple pear, respectively. We reported 44.7 35.7 Mb V. , also identified genomic determinants wood colonization comparing them with related cereal pathogens. Both...
Pathogen-associated molecular patterns (PAMPs) are conserved molecules that crucial for normal life cycle of microorganisms. However, the diversity microbial PAMPs is little known. During screening cell-death-inducing factors from necrotrophic fungus Valsa mali, we identified a novel PAMP VmE02 widely spread in oomycetes and fungi. Agrobacterium tumefaciens-mediated transient expression or infiltration recombinant protein produced by Escherichia coli was performed to assay elicitor activity...
Inhibiting pathogen chemotaxis is a promising strategy for reducing disease pressure. However, this currently in the proof-of-concept stage. Here, Phytophthora sojae was used as model, its biflagellated zoospores could sense genistein, soybean root exudate, to navigate host and initiate infection. We identify P. IRK1 (isoflavone-insensitive receptor kinase 1) with PsIRK2 functioning coreceptor that enhances binding affinity of PsIRK1 genistein regulates by phosphorylating G protein α...
Abstract Fungal diseases are posing tremendous threats to global economy and food safety. Among them, Valsa canker, caused by fungi of their Cytospora anamorphs, has been a serious threat fruit forest trees is one the most destructive apple in East Asia, particularly. Accurate robust delimitation pathogen species not only essential for development effective disease control programs, but also will advance our understanding emergence plant diseases. However, especially difficult because high...
Canker caused by the Ascomycete Valsa mali is most destructive disease of apple in Eastern Asia, resulting yield losses up to 100 %. This necrotrophic fungus induces severe necrosis on apple, eventually leading death whole tree. Identification inducing factors may help unravel molecular bases for colonization trees V. mali. As a first step towards this goal, we identified and characterized repertoire Candidate Effector Proteins (CEPs). In total, 193 secreted proteins with no known function...
Summary Phytoplasmas are insect‐transmitted phytopathogenic bacteria, which secrete effector proteins that often responsible for altering the plant morphology and behaviours of their vectors. Phytoplasma multifunctional TENGU SAP11 induce typical witches’ broom symptoms, but mode action remains unknown. Previously, we have identified a SAP11‐like from wheat blue dwarf phytoplasma, SWP1, induces symptoms in Nicotiana benthamiana . In this study, observed SWP1 ‐expressing transgenic...
Since the first plant receptor-like kinase (RLK) gene ZmPK1 was cloned from Zea mays in 1990 (Walker and Zhang, 1990Walker J.C. Zhang R. Relationship of a putative receptor protein maize to S-locus glycoproteins Brassica.Nature. 1990; 345: 743-746Crossref PubMed Google Scholar), this large family has been extensively studied shown play crucial roles growth, development, immunity (Tang et al., 2017Tang D. Wang G. Zhou J.M. Receptor Kinases Plant-Pathogen Interactions: More Than Pattern...
Rationale: Müller glia (MG) play a key role in maintaining homeostasis of the retinal microenvironment.In zebrafish, MG reprogram into progenitors and repair injured retina, while this regenerative capability is suppressed mammals.It has been revealed that microglia zebrafish contribute to reprogramming, whereas those mammals are over-activated during injury or degeneration, causing chronic inflammation, acceleration photoreceptor apoptosis, gliosis MG.Therefore, how modulate phenotype...
Due to the monocultural basis of agricultural crops, mutated plant microbes with increased pathogenicity can easily spread in field and lead serious yield losses. As a major threat wide range crop plants, oomycete pathogens continuously undergo adaptive evolution overcome defense barriers. However, genetic their at molecular level remains largely unknown. Here, we investigated nature variation population genomics soybean pathogen Phytophthora sojae by high-throughput genome re-sequencing....
Abstract Verticillium wilt diseases caused by the soil‐borne fungus dahliae result in devastating yield losses many economically important crops annually. Here, we identified a novel ethylene‐inducing xylanase (EIX)‐like protein, VdEIX3, from V. , which exhibits immunity‐inducing activity Nicotiana benthamiana . In vitro ‐purified VdEIX3 can induce strong oxidative burst, activate expression of defense‐related genes, and increase resistance against oomycete fungal pathogens N. orthologs...
Excessive light exposure can damage photoreceptors and lead to blindness. Oxidative stress serves a key role in photo-induced retinal damage. Free radical scavengers have been proven protect against photo-damaged degeneration. Fullerol, potent antioxidant, has the potential ultraviolet-B (UVB)-induced cornea injury by activating endogenous stem cells. However, its effects on cell fate determination of Müller glia (MG) between gliosis de-differentiation remain unclear. Therefore, we...
Apple Valsa canker, caused by the fungus mali, is one of most destructive diseases apple trees in East Asia. Feruloyl esterases (ferulic acid esterases, FAEs), which belong to a subclass carboxylic can cleave ester bonds that crosslink hydroxycinnamic acids and arabinoxylans or certain pectins plant cell walls. However, pathogenic role FAE has not been demonstrated plant-pathogenic fungi. In this study, gene family, including type A, B, three C two D genes, was identified V. mali. Five seven...
Apple Valsa canker is a destructive disease caused by the ascomycete mali and poses serious threat to apple production. Toxins synthesized secondary metabolite biosynthetic gene clusters (SMBGCs) have been proven be crucial for pathogen virulence. Previous study showed that V. genome contains remarkably expanded SMBGCs some genes of them were significantly up-regulated during infection. In this study, we focus on LaeA, known regulator metabolism, its role in regulation, toxin production,...
Phytophthora capsici is one of the most harmful pathogens in agriculture, which threatens safe production multiple crops and causes serious economic losses worldwide. Here, we identified a P. expansin-like protein, PcEXLX1, by liquid chromatography-tandem mass spectrometry from Nicotiana benthamiana apoplastic fluid infected with capsici. Clustered regularly interspaced short palindromic repeats/crispr associated protein 9 (CRISPR/Cas9)-mediated PcEXLX1 knockout mutants exhibited...
Receptor-like proteins (RLPs) lacking the cytoplasmic kinase domain play crucial roles in plant growth, development and immunity. However, what remains largely elusive is whether RLP protein levels are fine-tuned by E3 ubiquitin ligases, which employed receptor-like kinases for signaling attenuation. Nicotiana benthamiana NbEIX2 a leucine-rich repeat (LRR-RLP) that mediates fungal xylanase-triggered Here we show associates with an F-box NbPFB1, promotes degradation likely forming SCF ligase...
Velvet protein family members are important fungal-specific regulators which involved in conidial development, secondary metabolism and virulence. To gain a broader insight into the physiological functions of velvet Valsa mali, causes highly destructive canker disease on apple, we conducted functional analysis two (VmVeA VmVelB) via gene replacement strategy. Deletion mutants VmVeA VmVelB showed increased melanin production, conidiation sensitivity to abiotic stresses, but exhibited reduced...
Background/Aims: Retinitis pigmentosa (RP) is characterized by degeneration of photoreceptors, and there are currently no effective treatments for this disease. However, curcumin has shown neuroprotectant efficacy in a RP rat swine model, thus, may have neuroprotective effects Methods: Immunofluorescence staining, electroretinogram recordings, behavioral tests were used to analyze the underlying mechanism retinal 1 (rd1) mice. Results: The number apoptotic cells retina rd1 mice at postnatal...
Abstract Cell‐surface‐localized leucine‐rich‐repeat receptor‐like kinases (LRR‐RLKs) are crucial for plant immunity. Most LRR‐RLKs that act as receptors directly recognize ligands via a large extracellular domain (ECD), whereas LRR‐RLK serve regulators relatively small and contain fewer LRRs. Here, we identified using high‐throughput tobacco rattle virus (TRV)‐based gene silencing in the model Nicotiana benthamiana . We used cell‐death phenotype caused by INF1, an oomycete elicitin induces...
Abstract Background Unlike in lower vertebrates, Müller glia (MG) adult mammalian retinas lack the ability to reprogram into neurons after retinal injury or degeneration and exhibit reactive gliosis instead. Whether a transition MG cell fate from reprogramming would help preserve photoreceptors is still under exploration. Methods A mouse model of retinitis pigmentosa (RP) was established using lineage tracing mice by intraperitoneal injection sodium iodate (SI). The critical time point for...
Metformin (MET), derived from Galega officinalis, stands as the primary first-line medication for treatment of type 2 diabetes (T2D). Despite its well-documented benefits in mammalian cellular processes, functions and underlying mechanisms plants remain unclear. This study aimed to elucidate MET's role inducing plant immunity investigate associated mechanisms. To impact MET on enhancing immune responses, we conducted assays measuring defense gene expression, reactive oxygen species (ROS)...