A5100629211- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Mycotoxins in Agriculture and Food
- Enzyme Production and Characterization
- Botany and Plant Ecology Studies
- Ecology and Vegetation Dynamics Studies
- Seed Germination and Physiology
- Plant Taxonomy and Phylogenetics
- Soil Carbon and Nitrogen Dynamics
- Fluorine in Organic Chemistry
- Environmental and Agricultural Sciences
- Photosynthetic Processes and Mechanisms
- Plant responses to water stress
- Plant Reproductive Biology
- Plant Virus Research Studies
- Rangeland Management and Livestock Ecology
- Microbial Natural Products and Biosynthesis
- Plant Micronutrient Interactions and Effects
- Plant Ecology and Soil Science
- Light effects on plants
- Plant nutrient uptake and metabolism
- Glycosylation and Glycoproteins Research
Hangzhou Normal University
2024-2025
Chinese Academy of Sciences
2014-2024
Qinghai University
2020-2024
China Meat Research Centre
2024
Shenzhen Polytechnic
2024
Guilin University of Electronic Technology
2024
Shandong Agricultural University
2024
Tianjin Institute of Industrial Biotechnology
2012-2024
Second Affiliated Hospital of Nanchang University
2023-2024
Nanchang University
2023-2024
The most recent outbreak of 2019 novel coronavirus, named as COVID-19, caused pneumonia epidemic in Wuhan with 2121 deaths cases February 20th 2020. Identification effective antiviral agents to combat the coronavirus is urgently needed. Citrus fruit peel or wild citrus are rich flavonoids, and clinically documented for roles relief cough promotion digestive health. Therefore, fruits assumed possess antivirus activities enhance host immunity. A previous study found that hesperetin could act a...
Abstract Revealing the effects of drought stress on photosynthetic characteristics and yield naked oats ( Avena nuda L.) is significant for enhancing productivity oats. In this study, a potted experiment consisting four water levels was conducted in Bashang area Hebei Province, China. The period established as continual 8 days during jointing-heading stage. aims were to reveal impacts critical results showed that rate P n ), transpiration T r stomatal conductance G s ) decreased under all...
Plasma membrane NADPH oxidases are major producers of reactive oxygen species ( ROS ) in plant cells under normal growth and stress conditions. In the present study total activity rice transcription OsRbohA , which encodes an Oryza sativa plasma oxidase, were stimulated by drought. was expressed all tissues examined throughout development. Its mRNA upregulated a number factors, including heat, drought, salt, oxidative methyl jasmonate treatment. Compared with wild‐type WT ), ‐knockout mutant...
Leaf rolling is considered as one of the most important agronomic traits in rice breeding. It has been previously reported that SEMI-ROLLED LEAF 1 (SRL1) modulates leaf by regulating formation bulliform cells (Oryza sativa); however, regulatory mechanism underlying SRL1 yet to be further elucidated. Here, we report functional characterization a novel leaf-rolling mutant, curled and dwarf (cld1), with multiple morphological defects. Map-based cloning revealed CLD1 allelic SRL1, loses function...
B-box (BBX) proteins play important roles in plant growth regulation and development including photomorphogenesis, photoperiodic of flowering, responses to biotic abiotic stresses. In the present study we retrieved total 131 BBX members from five Poaceae species 36 maize, 30 rice, 24 sorghum, 22 stiff brome, 19 Millet. All genes were grouped into subfamilies on basis their phylogenetic relationships structural features. The expression profiles 12 OsBBX different tissues evaluated through...
Summary Phytoplasmas are insect‐transmitted phytopathogenic bacteria, which secrete effector proteins that often responsible for altering the plant morphology and behaviours of their vectors. Phytoplasma multifunctional TENGU SAP11 induce typical witches’ broom symptoms, but mode action remains unknown. Previously, we have identified a SAP11‐like from wheat blue dwarf phytoplasma, SWP1, induces symptoms in Nicotiana benthamiana . In this study, observed SWP1 ‐expressing transgenic...
As the key producers of reactive oxygen species (ROS), NADPH oxidases (NOXs), also known as respiratory burst oxidase homologs (RBOHs), play crucial roles in various biological processes plants with considerable evolutionary selection and functional diversity entire terrestrial plant kingdom. However, only limited resources are available on phylogenesis functions this gene family wheat. Here, a total 46 NOX genes were identified wheat genome, these NOXs could be classified into three...
Aquaculture feed containing olive oil (OO) instead of fish (FO) can cause oxidative stress and impair gonad development in fish. We determined the effect dietary OO-induced on ovarian development, explored whether vitamin E (VE) could mitigate negative effects. Female Nile tilapia (Oreochromis niloticus) were fed for 10 weeks with four diets: 5% OO + 70 mg/kg VE, 200 FO or VE. Dietary reduced specific growth rate gonadosomatic index, inhibited superoxide dismutase catalase, delayed decreased...
CRISPR-Cas systems endow the bacterial and archaeal species with adaptive immune mechanisms to fend off invading phages foreign plasmids. The class 2 type VI CRISPR/Cas effector Cas13d has been harnessed confer protection against RNA viruses in diverse eukaryotic species. However a vast number of different can potentially infect same host plant resulting mixed infection, thus necessitating generation crops broad-spectrum resistance multiple viruses. Here we report repurposing CRISPR/Cas13d...
A new, stable and scalable reagent based on a sulfoxide skeleton for direct deuteriodifluoromethylthiolation has been developed. The displays excellent reactivities toward Tf2O promoted C–H of electron-rich arenes, indoles, alkenes, intramolecular lactonization 2-alkynylbenzoates. Moreover, high deuteration rates good to yields were achieved under metal-free reaction conditions. As result, wide range deuteriodifluoromethylthilolated compounds prepared, enabling further applications in drug discovery.
Abstract Volatile organic compounds (VOCs) are a significant source of environmental pollution, posing threats to human safety. With growing concerns about degradation, catalytic oxidation technology emerges as paramount and widely adopted approach for VOC elimination, renowned its operational simplicity, energy efficiency, friendliness. As representative transition metal catalyst, cobalt‐based catalysts have garnered widespread use in due their cost‐effectiveness, versatility, distinctive...
Abstract Purpose The present study aims to determine the molecular mechanism mediated by RAD51 antisense RNA 1 (RAD51-AS1) in ovarian cancer (OvCA). Methods data associated with RAD51-AS1 OvCA were obtained from Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) database. Relative expression of was detected. Determination cell proliferation, metastasis, invasion performed counting, colony formation, would-healing, transwell assays. Protein levels detected western blotting....
We report the first X-ray crystal structure of ent-kaur-16-ene synthase from Bradyrhizobium japonicum, together with results a site-directed mutagenesis investigation into catalytic activity. The is very similar to that α domains modern plant terpene cyclases, result interest since it has been proposed many cyclases may have arisen bacterial diterpene cyclases. ent-copalyl diphosphate substrate binds hydrophobic pocket near cluster Asp and Arg residues are essential for catalysis,...
Zearalenone (ZEN) is a mycotoxin which causes huge economic losses in the food and animal feed industries. The lactonase ZHD101 from Clonostachys rosea, catalyzes hydrolytic degradation of ZEN, only known ZEN-detoxifying enzyme. Here, protein homologous to ZHD101, denoted CbZHD, Cladophialophora batiana was expressed characterized. Sequence alignment indicates that CbZHD possesses same catalytic triad ZEN-interacting residues as found ZHD101. exhibits optimal enzyme activity at 35°C pH 8,...
The enzyme ZHD101 from Clonostachys rosea hydrolyzes and deactivates the mycotoxin zearalenone (ZEN) its zearalenol (ZOL) derivatives. prefers ZEN to ZOL as substrate, but ZOL, especially α-form, shows higher estrogenic toxicity than ZEN. To enhance α-ZOL selectivity, we solved complex structures of with both ZOLs modified several lactone-surrounding residues. Among mutants, V153H maintained activity for showed a 3.7-fold increase in specific against α-ZOL, an ∼2.7-fold reduction substrate...
Development of potent biocatalysts for enzymatic detoxification estrogenic mycotoxin zearalenone (ZEN) and its more toxic derivative α-zearalenol (α-ZOL) is great interest. Here, we report the crystal structures a ZEN-hydrolyzing enzyme from Rhinocladiella mackenziei (RmZHD), including substrate complexes. A molecular mechanism distinct activity RmZHD in hydrolyzing structurally similar ZEN α-ZOL then proposed. In addition, structure-based engineering to modify substrate-binding pocket...