A5011892520- Renal cell carcinoma treatment
- Renal and related cancers
- Bladder and Urothelial Cancer Treatments
- Cancer Genomics and Diagnostics
- Prostate Cancer Treatment and Research
- Urinary and Genital Oncology Studies
- Cancer, Lipids, and Metabolism
- Cancer Immunotherapy and Biomarkers
- Prostate Cancer Diagnosis and Treatment
- Pediatric Urology and Nephrology Studies
- MRI in cancer diagnosis
- Urological Disorders and Treatments
- Urologic and reproductive health conditions
- Medical Imaging and Pathology Studies
- Liver physiology and pathology
- Multiple and Secondary Primary Cancers
- Pancreatic and Hepatic Oncology Research
- Cancer Diagnosis and Treatment
- Radiomics and Machine Learning in Medical Imaging
- Cancer, Hypoxia, and Metabolism
- Ferroptosis and cancer prognosis
- Nuclear Structure and Function
- Genital Health and Disease
- Renal and Vascular Pathologies
- Genetic and Kidney Cyst Diseases
Kanagawa Prefectural Hospital Organization
2012-2025
Kanagawa Cancer Center
2012-2025
Yokohama City University
2015-2024
Yokohama City University Hospital
2011-2023
Yokohama City University Medical Center
2006-2018
Laboratory of Molecular Genetics
2006-2007
Urology San Antonio
2005
National Cancer Institute
1997-2001
Frederick National Laboratory for Cancer Research
1999-2001
Cancer Research Center
2000
Recently, mutations in the Met tyrosine kinase receptor have been identified both hereditary and sporadic forms of papillary renal carcinoma. We introduced corresponding into met cDNA examined effect each mutation biochemical biological assays. find that mutants exhibit increased levels phosphorylation enhanced activity toward an exogenous substrate when compared with wild-type Met. Moreover, NIH 3T3 cells expressing mutant molecules form foci vitro are tumorigenic nude mice. Enzymatic...
Abstract BACKGROUND Advanced glycation end products (AGE) are produced with normal aging. Recently, some reports indicated that the interaction between AGE and cognate receptor (RAGE) has a role in cancer dependent. METHODS We investigated RAGE amphoterin mRNA expression prostate cell lines (DU145, PC‐3, LNCaP cells), hormone‐refractory tissues, paired untreated primary (including benign prostatic hypertrophy (BPH)) tissues using real‐time quantitative PCR. Moreover, to confirm AGE–RAGE...
Abstract To delineate more precisely the somatic von Hippel‐Lindau disease (VHL) gene alteration as well to elucidate its etiologic role in renal tumorigenesis, we examined a total of 240 sporadic cell carcinomas (RCCs) for VHL alterations by DNA‐SSCP followed sequencing, methylation‐specific PCR assay, microsatellite LOH study, and Southern blot analysis. Intragenic mutation was found exclusively clear‐cell or variant‐type RCCs at frequency 51% (104/202). Hypermethylation promoter region...
Abstract The gene expression profiles of 33 renal cell carcinomas (RCCs) and nine normal kidney samples were examined using high‐density oligonucleotide microarrays in an attempt to identify biomolecular markers for the diagnosis tumour subtypes also prediction prognosis. Hierarchical clustering demonstrated that clear‐cell RCC, chromophobe tissue showed distinctive profiles. mean levels 149 12 500 genes more than three times higher RCC tissue. Among whose was upregulated adipose...
Abstractβ-Catenin is an oncogenic protein involved in regulation of cell-cell adhesion and gene expression. Accumulation cellular β-catenin occurs many types human cancers. Four mechanisms are known to cause increases β-catenin: mutations β-catenin, adenomatous polyposis coli, or axin genes activation Wnt signaling. We report a new accumulation involving mutants RON MET receptor tyrosine kinases (RTKs). Cells transfected with were characterized by phosphorylation accumulation; constitutive...
Abstract Background In this era of molecular targeting therapy when various systematic treatments can be selected, prognostic biomarkers are required for the purpose risk-directed selection. Numerous reports malignancies have revealed that 18-Fluoro-2-deoxy-D-glucose ( 18 F-FDG) accumulation, as evaluated by positron emission tomography, used to predict prognosis patients. The study was evaluate impact maximum standardized uptake value (SUVmax) from 18-fluoro-2-deoxy-D-glucose...
// Takashi Kawahara 1, 2, * , Sachi Fukui Kentaro Sakamaki 3 Yusuke Ito 1 Hiroki Naohito Kobayashi Koji Izumi Yumiko Yokomizo Yasuhide Miyoshi 2 Kazuhide Makiyama Noboru Nakaigawa Takeharu Yamanaka Masahiro Yao Hiroshi Miyamoto 4 Hiroji Uemura Department of Urology, Yokohama City University, Graduate School Medicine, Yokohama, Japan Departments Urology and Renal Transportation, University Medical Center, Biostatistics, Pathology Johns Hopkins Baltimore, USA These authors have contributed...
Abstract It is well known that inactivation of von Hippel-Lindau (VHL) gene predisposes for human clear cell renal carcinoma (CCRC). However, details about critical roles VHL during tumorigenesis are still unknown. MET protein a tyrosine kinase receptor hepatocyte growth factor/scatter factor (HGF/SF), which regulates growth, morphology, and motility. We showed overexpressed in CCRC cells was phosphorylated without HGF/SF. This constitutive phosphorylation inhibited by the rescue exogenous...
Understanding the mechanism by which hormone refractory prostate cancer (HRPC) develops remains a major issue. Alterations in HRPC include androgen receptor (AR) changes. In addition, AR is activated cytokines such as interleukin-6 (IL-6). Atypical protein kinase C (aPKClambda/iota) has been implicated progression of several cancers. Herein, we provide evidence that aPKClambda/iota expression correlates with recurrence. Experiments vitro and vivo revealed to be involved cell growth through...
Abstract Background We reported previously that 18 F-2-fluoro-2-deoxyglucose positron emission tomography/ computed tomography (FDG PET/CT) had potential for evaluating early response to treatment by tyrosine kinase inhibitors (TKIs) in advanced renal cell carcinoma (RCC). This time we investigated the relation of assessment FDG PET/CT long-term prognosis with an expanded number patients and period observation. Methods Patients whom TKI RCC was planned were enrolled. performed before after...
Pancreatic ductal adenocarcinoma (PDAC) is a highly malignant tumor, for which the development of new biomarkers and therapeutic targets has become critical. The main cause poor prognosis in PDAC patients high invasive metastatic potential cancer. In present study, we report signaling pathway that was found to mediate enhanced tumor cell motility pancreatic Semaphorin 4D (Sema4D) ligand known be expressed on different types, been reported involved regulation immune functions, epithelial...
Various molecular-targeting therapies have become available for the treatment of advanced renal cell carcinoma (RCC). Accurate prognostication is desirable choosing appropriate individual patients. 18F-2-fluoro-2-deoxyglucose positron-emission tomography/computed tomography (FDG PET/CT) a non-invasive tool evaluating glucose accumulation, which can be an index biological characteristics cancer. We prospectively evaluated FDG PET/CT as prognostic indicator in patients with RCC. A total 101...
There is no reliable biomarker for predicting the prognosis of patients who undergo radical cystectomy bladder cancer. Recent studies have shown that neutrophil-to-lymphocyte ratio (NLR) could function as a useful prognostic factor in several types malignancies. This study aimed to assess usefulness NLR A total 74 underwent our institutions from 1999 2014 were analyzed. The was calculated using patients' neutrophil and lymphocyte counts before cystectomy. An immunohistochemical analysis also...
Bladder cancers have been characterized as a tumor group in which the immunological response is relatively well preserved. Programmed death ligand 1 (PD-L1, B7-H1, CD274) has shown to be expressed several malignancies, including bladder cancer. However, clinicopathological impact of this biomarker not yet established. In present study, quantitative real-time polymerase chain reaction (qPCR) was performed using paired normal and cancerous cancer tissue investigate PD-1/PD-L1 gene expression....
Recently, sarcopenia has been reported as a new predictor for patient outcomes or likelihood of post-operative complications. The purpose this study was to evaluate the association psoas muscle volume with length hospitalization among patients undergoing radical cystectomy.A total 63 (80.8%) male and 15 (19.2%) female who underwent cystectomy their bladder cancer in our institution from 2000 2015 were analyzed. index (PMI) calculated by normalizing area using axial computed tomography at...
Abstract We showed previously that angiotensin II activated the proliferation of prostate cancer cells and receptor blockers (ARB) could inhibit it. Here, we investigated whether exerts mitogenic effects on cross-talk between stromal an ARB can tumor growth through actions cells. Cell interleukin-6 secretion PrSC stimulated with II, necrosis factor-α, or epidermal factor were examined in absence presence ARB. effect mitogen-activated protein kinase (MAPK) phosphorylation PC-3 treated...
Abstract Purpose: We recently found that adipose differentiation-related protein (ADFP) is a potential diagnostic and prognostic biomarker for clear cell subtype renal carcinoma (RCC). To further evaluate the correlation between ADFP expression levels clinicopathologic characteristics patient outcome, we retrospectively examined patients with RCC. Experimental Design: A series of 432 consecutive sporadic RCC who underwent nephrectomy March 1986 June 2004 were enrolled in study. primary...
Abstract Purpose: Vascular cell adhesion molecule 1 (VCAM1) is a surface glycoprotein implicated in various pathophysiologic conditions. We measured VCAM1 expression levels tumor tissues and evaluated its significance prognostic use renal carcinoma (RCC). Experimental Design: used real-time quantitative PCR to examine the of total 485 sporadic tumors, including 429 clear cell, 21 papillary, 17 chromophobe, 11 oncocytomas, 7 collecting duct carcinomas. retrospectively examined relationship...
Abstract Renal cell carcinomas (RCCs) are morphologically and genetically heterogeneous tumors present diverse clinical courses. We developed a scoring system using levels of gene expression to predict the outcome for clear RCC patients. selected differentially expressed genes from DNA microarray data 27 RCCs; 16 were metastasis phenotypes 11 not. compared set with previously published identified 33 overlapping closely associated patient outcome. 12 top‐ranked confirmed level quantitative...