A5065600437- Nitric Oxide and Endothelin Effects
- Renal function and acid-base balance
- Eicosanoids and Hypertension Pharmacology
- Cardiac Ischemia and Reperfusion
- Cardiac and Coronary Surgery Techniques
- Advanced Glycation End Products research
- Cardiovascular Health and Disease Prevention
- Cardiac Valve Diseases and Treatments
- Blood Pressure and Hypertension Studies
- Sirtuins and Resveratrol in Medicine
- Cardiac Imaging and Diagnostics
- Organ Transplantation Techniques and Outcomes
- Urticaria and Related Conditions
- Calcium signaling and nucleotide metabolism
- Mast cells and histamine
- Cardiac electrophysiology and arrhythmias
- Natural Products and Biological Research
- Hepatitis B Virus Studies
- Hepatitis Viruses Studies and Epidemiology
- Adipose Tissue and Metabolism
- Healthcare during COVID-19 Pandemic
- Bioactive natural compounds
- Liver Disease and Transplantation
- Healthcare professionals’ stress and burnout
- Stress and Burnout Research
Universidade Federal de São Paulo
2020-2023
Instituto de Saúde
2020-2021
Universidade de São Paulo
2009-2020
Universidade Brasil
2008-2016
Mayo Clinic
2012-2014
Universidade de Ribeirão Preto
2011-2012
Departamento de Ciência e Tecnologia
2011
Universidade de Pernambuco
2010
Universidade Estadual Paulista (Unesp)
2006
Metabolic syndrome is a growing health problem worldwide. It therefore imperative to develop new strategies treat this pathology. In the past years, manipulation of NAD(+) metabolism has emerged as plausible strategy ameliorate metabolic syndrome. particular, an increase in cellular levels beneficial effects, likely because activation sirtuins. Previously, we reported that CD38 primary NAD(+)ase mammals. Moreover, knockout mice have higher and are protected against obesity Here, show...
We investigated the mechanism by which extracellular acidification promotes relaxation in rat thoracic aorta. The response to HCl-induced (7.4 6.5) was measured aortic rings pre-contracted with phenylephrine (Phe, 10− 6 M) or KCl (45 mM). vascular reactivity experiments were performed endothelium-intact and denuded rings, presence absence of indomethacin (10− 5 M), L-NAME 4 apamin glibenclamide M). effect acidosis (pH 7.0 on nitric oxide (NO) production evaluated isolated endothelial cells...
Aims It has been known for more than a century that pH changes can alter vascular tone. However, there is no consensus about the effects of on response. In this study, we investigated extracellular (pHo) intracellular (pHi) and nitric oxide concentration ([NO]i) in freshly isolated endothelial cells cross sections from rat aorta. Main Methods The HCl was used to reduce pHo 7.4 7.0 6.5; NaOH increase 8.0 8.5. fluorescent dyes 5-(and-6)-carboxy SNARF-1, acetoxymethyl ester, acetate (SNARF-1)...
Hepatic ischemia followed by reperfusion (IR) results in mild to severe remote organ injury. Oxidative stress and nitric oxide (NO) seem be involved the IR Our aim was investigate effects of liver I/R on hepatic function lipid peroxidation, leukocyte infiltration NO synthase (NOS) immunostaining lung kidney. We randomized 24 male Wistar rats into 3 groups: 1) control; 2) 60 minutes partial (70%) I 2 hours global R; 3) 6 R. Groups showed significant increases plasma alanine aspartate...
The Temporomandibular disorder (TMD) is greatly prevalent in the population and can be associated with bruxism. This produces several signs symptoms. Among them, pain one of most important because it reduces life quality productivity people who have such disorder. aim this research was to study if massage causes relief and/or electromyographic (EMG) changes.The subjects were chosen by a questionnaire divided into 2 different groups. Their ages varied from 19 22 years. experimental group...
This study aimed to evaluate the stress perception among Brazilian physical therapists (PTs) during COVID-19 pandemic and identify which psychosocial demands, sociodemographic, professional clinical factors do associate with PTs' perception. cross-sectional survey was based on a convenience sample of PTs, who answered questionnaire about: 1) sociodemographic characteristics, 2) characteristics information related COVID-19, 3) 4) 10-item Perceived Stress Scale (PSS-10). Full responses were...
Metabolic acidosis has profound effects on vascular tone. This study investigated the in vivo of acute metabolic (AMA) and chronic (CMA) hemodynamic parameters endothelial function. CMA was induced by ad libitum intake 1% NH4Cl for 7 days, AMA a 3-h infusion 6 M (1 mL/kg, diluted 1:10). Phenylephrine (Phe) acetylcholine (Ach) dose-response curves were performed venous with simultaneous arterial blood pressure monitoring. Plasma nitrite/nitrate (NOx) measured chemiluminescence. The group had...
Abstract Background and Aim: There were strong evidences that nitric oxide has capital importance in the progressive vasodilatation associated with varied circulatory shock forms, including systemic inflammatory response syndrome (SIRS), patients undergoing cardiac surgeries for cardiopulmonary bypass (CPB). If CPB procedures, per se, are inciting stimulus inflammation, plasma nitrate/nitrite (NOx) excretion would be expected to higher these rather than operated without CPB. In consequence,...
OBJECTIVES:The clinical significance of ischemia/reperfusion the lower extremities demands further investigation to enable development more effective therapeutic alternatives.This study investigated changes in vascular reactivity rabbit femoral artery and nitric oxide metabolites under partial ischemia/ reperfusion conditions following cilostazol administration.METHODS: Ischemia was induced using infrarenal aortic clamping.The animals were randomly divided into seven groups: Control 90...
To investigate the effects of chronic ethanol consumption and diabetes on nitric oxide (NO)-mediated relaxation cavernosal smooth muscle (CSM).Male Wistar rats were divided into four groups: control, isocaloric, diabetic ethanol-diabetic. The CSMs mounted in organ chambers for measurement isometric tension. Contraction strips was induced by electrical field stimulation (EFS, 1-32 Hz) phenylephrine. We also evaluated effect acetylcholine (ACh; 0.01-1000 micromol/L), sodium nitroprusside (SNP,...
OBJECTIVES: To verify if an experimental model of alloxan-diabetic rats promotes oxidative stress, reduces nitric oxide bioavailability and causes vascular dysfunction, to evaluate the effect N-acetylcysteine (NAC) on these parameters. METHODS: Alloxan-diabetic were treated or not with NAC for four weeks. Plasmatic levels malondialdehyde (MDA) nitrite/nitrate (NOx), endothelial inducible synthase (eNOS iNOS) immunostaining reactivity aorta compared among diabetic (D), (TD) control (C) rats....
To verify if the methylene blue (MB) administration prevents and/or reverses compound 48/80 (C48/80)-induced anaphylactic shock in pigs.Female Dalland pigs were anesthetized and had hemodynamic parameters recorded during necessary time to administer some drugs observe their effect. The animals randomly assigned one of five groups: 1) control; 2) MB: received a bolus injection MB (2 mg/kg) followed by continuous infusion (2.66 mg/Kg/h delivered syringe pump); 3) C48/80: C48/80 (4 mg/kg); 4)...
To investigate the mechanism through which extracellular alkalinization promotes relaxation in rat thoracic aorta.The response to NaOH-induced (7.4-8.5) was measured aortic rings pre-contracted with phenylephrine (Phe, 10(-6) M). The vascular reactivity experiments were performed endothelium-intact and -denuded rings, presence or absence of indomethacin (10(-5) M), NG-nitro-l-arginine methyl ester (L-NAME, 10(-4) N-(6-Aminohexyl)-5-chloro-1-naphthalenesulfonamide/HCl (W-7, 10(-7)...
Abstract Background The supraceliac aortic cross-clamping can be an option to save patients with hipovolemic shock due abdominal trauma. However, this maneuver is associated ischemia/reperfusion (I/R) injury strongly related oxidative stress and reduction of nitric oxide bioavailability. Moreover, several studies demonstrated impairment in relaxation after I/R, but the time course I/R necessary induce vascular dysfunction still controversial. We investigated whether 60 minutes ischemia...
<b><i>Background and Purpose:</i></b> There is a remarkable paucity of studies analyzing the role endothelium-derived relaxing factors on vascular effects organophosphates. This study was carried out to evaluate malathion nitric oxide (NO) prostacyclin (PGI<sub>2</sub>). <b><i>Methods:</i></b> Vascular reactivity measuring isometric forces in vitro (‘organ chambers') flow cytometry (cells loaded with DAF-FM DA) were used....