A5055555751- Prion Diseases and Protein Misfolding
- RNA Research and Splicing
- Alzheimer's disease research and treatments
- Metabolism and Genetic Disorders
- Plant tissue culture and regeneration
- RNA and protein synthesis mechanisms
- Mitochondrial Function and Pathology
- RNA regulation and disease
- Neurological diseases and metabolism
- Fungal and yeast genetics research
- Amino Acid Enzymes and Metabolism
- Trace Elements in Health
- Plant Genetic and Mutation Studies
- Plant Reproductive Biology
- Transgenic Plants and Applications
- Chromosomal and Genetic Variations
- Evolution and Genetic Dynamics
- Plant Molecular Biology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Protein Structure and Dynamics
- Proteins in Food Systems
- Liver Disease Diagnosis and Treatment
- Heat shock proteins research
- Chemical Synthesis and Analysis
- Effects of Radiation Exposure
St Petersburg University
2016-2025
Russian Academy of Sciences
2008-2025
Vavilov Institute of General Genetics
2014-2024
Institute of Food Biotechnology and Genomics
2016
National Academy of Sciences of Ukraine
2016
N.I. Vavilov Research Institute of Plant Industry
2012
University of Helsinki
2006
Institute of Bioorganic Chemistry and Petrochemistry V.P. Kukhar
1993-2004
National Institutes of Health
2002
National Center for Biotechnology Information
2002
Saccharomyces cerevisiae prion [PSI ] is a self-propagating isoform of the eukaryotic release factor eRF3 (Sup35p). Sup35p consists evolutionary conserved domain (Sup35C) and two variable regions - Sup35N, which serves as prion-forming in S. cerevisiae, Sup35M. Here, we demonstrate that form not observed among industrial natural strains yeast. Moreover, ([PSI + ]) state endogenous cannot be transmitted to next generations via heterologous or Sup35NM, originating from distantly related yeast...
Abstract Dysfunctional RNA-binding proteins (RBPs) have been implicated in several neurodegenerative disorders. Recently, this paradigm of RBPs has extended to pathophysiology Alzheimer’s disease (AD). Here, we identified subtype specific variations the proteome (RBPome) sporadic AD (spAD), rapidly progressive (rpAD), and Creutzfeldt Jakob (sCJD), as well control cases using RNA pull-down assay combination with proteomics. We show that one these proteins, splicing factor proline glutamine...
To test the hypothesis that inaccurate DNA synthesis by mammalian polymerase eta (pol eta) contributes to somatic hypermutation (SHM) of Ig genes, we measured error specificity mouse pol during each strand a kappa light chain transgene. We then compared results base substitution SHM this same gene in mouse. The vitro and vivo spectra shared number common features. A highly significant correlation was observed for overall substitutions at A-T pairs but not G-C pairs. Sixteen mutational...
Despite extensive study, progress in elucidation of biological functions amyloids and their role pathology is largely restrained due to the lack universal reliable biochemical methods for discovery. All developed so far allowed only identification glutamine/asparagine-rich amyloid-forming proteins or comprising that form large deposits. In this article we present a proteomic approach which may enable broad range independently specific features sequences levels expression. This based on...
The concept of "protein-based inheritance" defines prions as epigenetic determinants that cause several heritable traits in eukaryotic microorganisms, such Saccharomyces cerevisiae and Podospora anserina. Previously, we discovered a non-chromosomal factor, [NSI+], which possesses the main features yeast prions, including cytoplasmic infectivity, reversible curability, dominance, non-Mendelian inheritance meiosis. This factor causes omnipotent suppression nonsense mutations strains S. bearing...
Abstract Amyloids are β-sheets-rich protein fibrils that cause neurodegenerative and other incurable human diseases affecting millions of people worldwide. However, a number proteins is functional in the amyloid state various organisms from bacteria to humans. Using an original proteomic approach, we identified set forming amyloid-like aggregates brain young healthy rats. One them FXR1 protein, which known regulate memory emotions. We showed clearly colocalizes cortical neurons with...
The severe course of COVID-19 causes systemic chronic inflammation and thrombosis in a wide variety organs tissues. nature these inflammations remains mystery, although they are known to occur against the background high level cytokine production. cytokines provokes overproduction Serum amyloid A (SAA) protein. Moreover, number studies has shown that SAA overproduction. authors works regard exclusively as biomarker COVID-19. However, it should be borne mind can cause AA amyloidosis. forms...
The FXR1 protein regulates the stability and translation of a number RNA molecules plays an important role in regulation cellular processes under normal conditions stress. In particular, this is known to be negative regulator key proinflammatory cytokine TNF alpha. We had previously shown that functioned amyloid form neurons brain jawed vertebrates. Under stress conditions, incorporated into granules some cell lines, but such studies have not been conducted for neuronal cells. Here, we...
Amyloids are fibrillar proteins with a cross-β structure. Pathological amyloids associated the development of number incurable diseases, while functional regulate vital processes. The detection unknown in living objects is difficult task, and therefore question prevalence biological significance remains open. We present description two methods, combination which makes it possible to find identify amyloid proteome various organisms. method proteomic screening for allows that form...
Alzheimer disease is associated with the accumulation of oligomeric amyloid β peptide (Aβ), accompanied by synaptic dysfunction and neuronal death. Polymeric form prion protein (PrP), PrP(Sc), implicated in transmissible spongiform encephalopathies (TSEs). Recently, it was shown that monomeric cellular PrP (PrP(C)), located on neuron surface, binds Aβ oligomers (and possibly other β-rich conformers) via PrP(23-27) PrP(90-110) segments, acting as receptor. On hand, PrP(Sc) polymers...
Amyloids are non-branching fibrils that composed of stacked monomers stabilized by intermolecular β-sheets. Some amyloids associated with incurable diseases, whereas others, functional amyloids, regulate different vital processes. The prevalence and significance in wildlife still poorly understood. In recent years, applying new approach large-scale proteome screening, a number novel candidate were identified the yeast Saccharomyces cerevisiae, many which localized cell wall. this work, we...
ABSTRACT We conducted a cytological search for amyloid structures in female reproductive cells of Gallus gallus domesticus and Drosophila melanogaster. have shown that the amyloid-specific dye, Thioflavin S, but not Congo red, stains some cytoplasmic even nuclear chicken ovaries. In fruit fly eggs both S red specifically stain eggshell such as micropyle, dorsal appendages pillars. Moreover, these structures, when stained with demonstrate birefringence polarized light, which is characteristic...
Linum usitatissimum L. (flax or linseed) is one of the oldest economically important crops used in biotechnology as a source for bioactive compounds. Flax fiber and seed have industrial significance cellulosic textile paper industry oil pharmaceutical, cosmetic food industry. The elaboration new effective techniques vitro regeneration very actual problem today. influence low molecular weight five sixmembered nitrogen-containing heterocyclic compounds on organogenesis cultivar heavenly has...