A5046040828- RNA Research and Splicing
- Neuroscience and Neuropharmacology Research
- Pain Mechanisms and Treatments
- Neurogenetic and Muscular Disorders Research
- Transcranial Magnetic Stimulation Studies
- Parkinson's Disease Mechanisms and Treatments
- Advanced Electron Microscopy Techniques and Applications
- Alzheimer's disease research and treatments
- Electron and X-Ray Spectroscopy Techniques
- Protein Structure and Dynamics
- Nerve injury and regeneration
- Prion Diseases and Protein Misfolding
- Neural and Behavioral Psychology Studies
- Genetic Neurodegenerative Diseases
- Stress Responses and Cortisol
- Vestibular and auditory disorders
- Virus-based gene therapy research
- Ion channel regulation and function
- Cholinesterase and Neurodegenerative Diseases
- RNA regulation and disease
Medical University of Vienna
2020-2025
German Center for Neurodegenerative Diseases
2020-2021
Universitätsmedizin Göttingen
2021
Bühler (United Kingdom)
2020
Creative Commons
2020
Abstract In Parkinson's disease with dementia, up to 50% of patients develop a high number tau‐containing neurofibrillary tangles. Tau‐based pathologies may thus act synergistically the α‐synuclein pathology confer worse prognosis. A better understanding relationship between two distinct is therefore required. Liquid–liquid phase separation (LLPS) proteins has recently been shown be important for protein aggregation involved in amyotrophic lateral sclerosis, whereas tau linked Alzheimer's...
Abstract Dysfunctional RNA-binding proteins (RBPs) have been implicated in several neurodegenerative disorders. Recently, this paradigm of RBPs has extended to pathophysiology Alzheimer’s disease (AD). Here, we identified subtype specific variations the proteome (RBPome) sporadic AD (spAD), rapidly progressive (rpAD), and Creutzfeldt Jakob (sCJD), as well control cases using RNA pull-down assay combination with proteomics. We show that one these proteins, splicing factor proline glutamine...
The spinal cord dorsal horn (DH) is essential for processing and transmitting nociceptive information. Its neuronal subpopulations exhibit significant heterogeneity in morphology intrinsic properties, forming complex circuits that remain only partially understood. Under physiological pathological conditions, inhibitory interneurons the DH are of particular interest. These neurons modulate refine pain-related signals entering central nervous system. ability to selectively target these key...
Abstract Withdrawal from systemic opioids can induce long-term potentiation (LTP) at spinal C-fibre synapses (“opioid-withdrawal-LTP”). This is considered to be a cellular mechanism underlying opioid withdrawal-induced hyperalgesia, which major symptom of the withdrawal syndrome. Opioids activate glial cells leading release proinflammatory mediators. These may influence synaptic plasticity and could thus contribute opioid-withdrawal-LTP. Here, we report sexual dimorphism in mechanisms...
Astrocytes are indispensable for proper neuronal functioning. Given the diverse needs of circuits and variety tasks astrocytes perform, perceived homogeneous nature has been questioned. In spinal dorsal horn, complex circuitries regulate integration sensory information different modalities. The horn is organized in a distinct laminar manner based on termination patterns high- low-threshold afferent fibers properties. Neurons laminae I (L1) II (L2) integrate potentially painful, nociceptive...
The molecular determinants of atypical clinical variants Alzheimer's disease, including the recently discovered rapidly progressive disease (rpAD), are unknown to date. Fibrilization amyloid-β (Aβ) peptide is most frequently studied candidate in this context. Aβ can exist as multiple proteoforms that vary their post-translational processing, amyloidogenesis, and toxicity. current study was designed identify these variations patients exhibiting classical (sAD) rapid progression, with primary...
Here we provide a protocol for cryo-electron tomography (cryo-ET) of thinned synapses within intact rat primary neuron cultures. This workflow relies on cryo-focused-ion-beam (FIB) milling to enable unrestricted access neuronal cultures and achieve samples sufficiently thin (~150 nm) high resolution cryo-ET imaging. allows targeting with without cryo-fluorescence light microscopy (cryo-FLM) correlation FIB cryo-ET.