A5053295842- Insect and Pesticide Research
- Antimicrobial Peptides and Activities
- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Healthcare and Venom Research
- Biochemical and Molecular Research
- Venomous Animal Envenomation and Studies
- Food Allergy and Anaphylaxis Research
- Biochemical and Structural Characterization
- Plant and animal studies
- Enzyme Structure and Function
- Allergic Rhinitis and Sensitization
- Ion channel regulation and function
- Lipid Membrane Structure and Behavior
- Silk-based biomaterials and applications
- HIV/AIDS drug development and treatment
- Cytomegalovirus and herpesvirus research
- Microbial Natural Products and Biosynthesis
- Bee Products Chemical Analysis
- Insect Utilization and Effects
- Nicotinic Acetylcholine Receptors Study
- Chemical Synthesis and Analysis
- Polyamine Metabolism and Applications
- Contact Dermatitis and Allergies
- Adenosine and Purinergic Signaling
Universidade Estadual Paulista (Unesp)
2016-2025
Universidade de São Paulo
2014-2025
University of Concepción
2022
Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular
2010-2012
Methodist University of Piracicaba
2012
National Council for Scientific and Technological Development
2004-2011
Universidade Estadual de Campinas (UNICAMP)
2010
Universidade Federal do Rio de Janeiro
2010
Fundação Oswaldo Cruz
2010
Universidade Federal do Rio Grande do Sul
2004-2008
Thiazolidinediones (TZDs) act through peroxisome proliferator activated receptor (PPAR) γ to increase insulin sensitivity in type 2 diabetes (T2DM), but deleterious effects of these ligands mean that selective modulators with improved clinical profiles are needed. We obtained a crystal structure PPARγ ligand binding domain (LBD) and found the pocket (LBP) is occupied by bacterial medium chain fatty acids (MCFAs). verified MCFAs (C8–C10) bind LBD vitro showed they low-potency partial agonists...
Polybia-MP1 (MP1) is a bioactive host-defense peptide with known anticancer properties. Its activity attributed to excess serine (phosphatidylserine (PS)) on the outer leaflet of cancer cells. Recently, higher quantities phosphatidylethanolamine (PE) were also found at these cells' surface. We investigate interaction MP1 model membranes in presence and absence POPS (PS) DOPE understand role lipid composition MP1's characteristics. Indeed we find that PS lipids significantly enhance bound...
The peroxisome proliferator-activated receptor γ (PPARγ) is a target for treatment of type II diabetes and other conditions. PPARγ full agonists, such as thiazolidinediones (TZDs), are effective insulin sensitizers anti-inflammatory agents, but their use limited by adverse side effects. Luteolin flavonoid with actions that binds but, unlike TZDs, does not promote adipocyte differentiation. However, previous reports suggested variously luteolin agonist or an antagonist. We show exhibits weak...
Fire ants are well-known by their aggressive stinging behavior, causing many incidents of medical importance. The limited availability fire ant venom for scientific and clinical uses has restricted, up to now, the knowledge about biochemistry, immunology, pharmacology these venoms. For this study, S. invicta was obtained commercially used proteomic characterization. purpose, combination gel-based gel-free strategies assign profile from invicta. This experimental approach permitted...
Victims of massive bee attacks become extremely ill, presenting symptoms ranging from dizziness and headache to acute renal failure multiple organ that can lead death. Previous attempts develop specific antivenom treat these victims have been unsuccessful. We herein report a F(ab)´2-based raised in horse as potential new treatment for stings. The final product contains high IgG titers is effective neutralizing toxic effects, such hemolysis, cytotoxicity myotoxicity. assessment neutralization...
Inflammatory peptides display different types of post-transcriptional modifications, such as C-terminal amidation, that alter their biological activity. Here we describe the structural and molecular dynamics features mast cell degranulating peptide, eumenine mastoparan-AF (EMP-AF-NH2), found in venom solitary wasp, its carboxyl-free form (EMP-AF-COO-) characterized by a reduced Circular dichroism indicates both switch from random coil conformation water to helical structure TFE SDS micelles....
Hymenoptera venoms are complex mixtures of biochemically and pharmacologically active components such as biogenic amines, peptides proteins. Polycationic generally constitute the largest group venom toxins, mastoparans most abundant important class in social wasps. These toxins responsible for histamine release from mast cells, serotonin platelets, catecholamines adenylic acids adrenal chromafin cells. The present work reports structural functional characterization two novel mastoparan...
Two-dimensional gel electrophoresis (2-DE) was used to better understand alterations in renal metabolism induced by fluoride (F). Three groups of weanling male Wistar rats were treated with drinking water containing 0 (control), 5, or 50 ppm F for 60 days (n = 6/group). Kidneys collected proteomic and histological (HE) analysis. After protein isolation, proteome profiles examined using 2-DE Colloidal Coomassie Blue staining. Protein spots a 2-fold significant difference as detected...
The study reported here is a classical bottom-up proteomic approach where proteins from wasp venom were extracted and separated by 2-DE; the individual protein spots proteolytically digested subsequently identified using tandem mass spectrometry database query with search engine MASCOT. Eighty-four belonging to 12 different molecular functions identified. These classified into three groups; first constituted of typical proteins: antigens-5, hyaluronidases, phospholipases, heat shock...
Antimicrobial peptides of the mastoparans family exert their bactericidal activity by binding to lipid membranes, inducing pores or defects and leaking internal contents vesicles cells. However, this does not seem be only mechanism at play, they might important in search for improved with lower undesirable side effects. This work deals three peptides, Polybia-MP-1(MP-1), N2-Polybia-MP-1 (N-MP-1), Mastoparan X (MPX), which exhibit high sequence homology. They all have lysine residues amidated...
Polycationic peptides may present their C-termini in either amidated or acidic form; however, the effects of these conformations on mechanisms interaction with membranes general were not properly investigated up to now. Protonectarina-MP mastoparan an C-terminus was utilized study interactions anionic and zwitterionic vesicles, using measurements dye leakage a combination H/D exchange mass spectrometry monitor peptide–membrane interactions. Mast cell degranulation, hemolysis antibiosis...
Biopharmaceutical drugs are mainly recombinant proteins produced by biotechnological tools. The patents of many biopharmaceuticals have expired, and biosimilars thus currently being developed. Human granulocyte colony stimulating factor (hG-CSF) is a hematopoietic cytokine that acts on cells the neutrophil lineage causing proliferation differentiation committed precursor activation mature neutrophils. Recombinant hG-CSF has been in genetically engineered Escherichia coli (Filgrastim)...