A5045481829- Developmental Biology and Gene Regulation
- Microtubule and mitosis dynamics
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- Epigenetics and DNA Methylation
- Congenital heart defects research
- Animal Genetics and Reproduction
- Ubiquitin and proteasome pathways
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- MicroRNA in disease regulation
- Hedgehog Signaling Pathway Studies
- Congenital limb and hand anomalies
- Cancer-related gene regulation
- Wnt/β-catenin signaling in development and cancer
- Protein Degradation and Inhibitors
- TGF-β signaling in diseases
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- Bone health and treatments
- RNA modifications and cancer
- Neurogenesis and neuroplasticity mechanisms
- Genetics, Bioinformatics, and Biomedical Research
- Mitochondrial Function and Pathology
- Cell Adhesion Molecules Research
University of Modena and Reggio Emilia
2012-2025
University of Perugia
2013
Cornell University
2005-2006
Vita-Salute San Raffaele University
1992-2004
San Raffaele University of Rome
1994-1999
Expression Génétique Microbienne
1999
The San Raffaele Telethon Institute for Gene Therapy
1998
Istituto Superiore di Sanità
1986-1989
Nuclear localization of the Extradenticle (EXD) and PBX1 proteins is regionally restricted during Drosophila mammalian development. We studied subcellular EXD, PBX, their partners Homothorax (HTH) PREP1, in different cell contexts. HTH PREP1 are cytoplasmic require association with EXD/PBX for nuclear localization. EXD murine fibroblasts but not Schneider cells, which they actively exported to cytoplasm. Coexpression HTH/PREP1 causes heterodimers both propose that heterodimerization HTH/PREP...
Vertebrate limb development occurs along three cardinal axes-proximodistal, anteroposterior and dorsoventral-that are established via the organization of signaling centers, such as zone polarizing activity (ZPA). Distal development, in turn, requires a molecular feedback loop between ZPA expression sonic hedgehog (Shh) apical ectodermal ridge. The TALE homeoprotein Pbx1 has been shown to be essential for proximal development. In this study, we first uncover that Pbx2 co-expressed lateral...
Glioblastoma multiforme (grade IV glioma) is characterized by a high invasive potential, making surgical intervention extremely challenging and patient survival very limited. Current pharmacological approaches show, at best, slight improvements in the therapy against this type of tumor. Microtubules are often target antitumoral drugs, specific drugs affecting their dynamics acting on microtubule-associated proteins (MAPs) without producing depolymerization could affect both glioma cell...
Transcription of human HOX gene promoters in cultured cells is positively and negatively regulated by proteins interacting with specific target sequences. The HOXD9 protein activates transcription the promoter HCR sequence antagonized HOXD8 protein. not intrinsically a repressor, since it can activate on different targets. Complete or partial HOXD8/HOXD9 homeo domain swapping indicates that ability to recognize, from, vivo depends amino terminus helix 1 domain. inhibitory activity affected...
Two human cDNA clones (HHO.c1.95 and HHO.c8.5111) containing a homeobox region have been characterized, the respective genomic regions partially analyzed. Expression of corresponding genes, termed c1 c8, was evaluated in different organs body parts during embryonic/fetal development. HHO.c1.95 apparently encodes 217-amino acid protein class I homeodomain that shares 60 out 61 amino residues with Antennapedia Drosophila melanogaster. HHO.c8.5111 153-amino identical to frog AC1 gene. Clones...
The genetic pathways underlying shoulder blade development are largely unknown, as gene networks controlling limb morphogenesis have limited influence on scapula formation. Analysis of mouse mutants for Pbx and Emx2 genes has suggested their potential roles in girdle development. In this study, by generating compound mutant mice, we examined the control functional relationship with Emx2. Analyses Pbx1;Emx2 revealed that share overlapping functions Pbx1 genetically interacts process. Here,...
Aberrant splicing events are associated with colorectal cancer (CRC) and provide new opportunities for tumor diagnosis treatment. The expression of the splice variants NF-YA, DNA binding subunit transcription factor NF–Y, is deregulated in multiple types compared to healthy tissues. NF–YAs NF–YAl isoforms differ transactivation domain, which may result distinct transcriptional programs. In this study, we demonstrated that transcript higher aggressive mesenchymal CRCs predicts shorter...
Abstract Background Glioblastoma (GBM) is the most lethal primary brain tumor in adult, characterized by highly aggressive and infiltrative growth. The current therapeutic management of GBM includes surgical resection followed ionizing radiations chemotherapy. Complex dynamic interplay between cells microenvironment drives progression contributes to resistance. Extracellular vesicles (EVs) play a crucial role intercellular communication delivering bioactive molecules surrounding milieu...
We report the cloning, genomic localization, primary structure and developmental expression pattern of novel mouse Hox-4.3 gene. This gene is located within HOX-4(5) complex, at a position which classifies it as member Hox-3.1 -2.4 subfamily, DNA predicted protein sequences further confirmed this classification. has characteristic Hox but, in addition, contains several monotonic stretches amino acids, one 'paired'-like type. As expected from its presence complex. developmentally expressed...
In Drosophila , the product of fs (2)cup gene (Cup) is known to be crucial for diverse aspects female germ-line development. Its functions at molecular level, however, have remained mainly unexplored. Cup was found directly associate with eukaryotic translation initiation factor 4E (eIF4E). this report, we show that a nucleocytoplasmic shuttling protein and interaction eIF4E promotes retention in cytoplasm. required correct accumulation localization within posterior cytoplasm developing...
Regionally restricted expression patterns of Hox genes in developing embryos rely on auto-, cross-, and para-regulatory transcriptional elements. One example is the Hoxb1 auto-regulatory element (b1-ARE), which drives fourth rhombomere hindbrain. We previously showed that HOXB1 PBX1 activate transcription from b1-ARE by binding to sequences required for a reporter gene 4 vivo. now report embryonal carcinoma cells, retain characteristics primitive neuroectodermal displays higher basal...