Plasmonic substrates play a crucial role in the confinement and manipulation of localized electromagnetic fields at nanoscale. The large field enhancement metal/dielectric interfaces is widely exploited surface-enhanced fluorescence (SEF) Raman scattering (SERS) spectroscopies. Despite advantage near-field enhancement, unfortunately, metals, absorption optical frequencies induces local heating analyte fluid with possible damage biological material. In addition, SEF plasmonic substrates,...

Abstract Acute lymphoblastic leukemia type B (B-ALL) is a neoplastic disorder that shows high mortality rates due to immature lymphocyte B-cell proliferation. B-ALL diagnosis requires identification and classification of the cells. Here, we demonstrate use Raman spectroscopy discriminate normal lymphocytic B-cells from three different B-leukemia transformed cell lines (i.e., RS4;11, REH, MN60 cells) based on their biochemical features. In combination with immunofluorescence Western blotting,...

In this paper we report on the engineering of repeatable surface enhanced Raman scattering (SERS) optical fiber sensor devices (optrodes), as realized through nanosphere lithography. The Lab-on-Fiber SERS optrode consists polystyrene nanospheres in a close-packed arrays configuration covered by thin film gold tip. surfaces were fabricated using lithography approach that is already demonstrated able to produce highly patterns order engineer and optimize probes, first evaluated compared...

Abstract The small molecule Galunisertib (LY2157299, LY) shows multiple anticancer activities blocking the transforming growth factor‐β1 receptor, responsible for epithelial‐to‐mesenchymal transition (EMT) by which colorectal cancer (CRC) cells acquire migratory and metastatic capacities. However, frequent dosing of LY can produce highly toxic metabolites. Alternative strategies to reduce drug side effects rely on nanoscale delivery systems that have led a medical revolution in treatment...

We developed an immunoassay platform for the detection of human Thyroglobulin (Tg) to be integrated with fine-needle aspiration biopsy early lymph node metastases in thyroid cancer patients. The sensing detects Tg by a sandwich involving self-assembled surface-enhanced Raman scattering (SERS) substrate assisted functionalized gold nanoparticles that provide additional signal amplification and improved molecular specificity. Specifically, SERS-active substrates were Capture antibodies...

The analysis of leukocytes peripheral blood is a crucial step in hematologic exams commonly used for disease diagnosis and, typically, requires molecular labelling. In addition, only detailed, laborious phenotypic allows identifying the presence and stage specific pathologies such as leukemia. Most biochemical information lost routine tests. present study, we tackle 2 important issues label-free identification classification using Raman spectroscopy (RS). First, demonstrate that leukocyte...

Diatoms can represent the major component of phytoplankton and contribute massively to global primary production in oceans. Over tens millions years they developed an intricate porous silica shell, frustule, which ensures mechanical protection, sorting nutrients from harmful agents, optimization light harvesting. Several groups microalgae evolved different strategies protection towards ultraviolet radiation (UVR), is for all living organisms mainly through formation dimeric photoproducts...

Porous biosilica nanoparticles obtained from diatomites (DNPs) have been recently demonstrated to be non‐toxic nanovectors of therapeutic agents in cancer cells. In this work, the internalization kinetics and intracellular spatial distribution functionalized DNPs incubated with human lung epidermoid carcinoma cell line (H1355) up 72 hours are investigated by Raman imaging. The label‐free results compared confocal fluorescence microscopy photoluminescence (PL) data. bands specifically...

In this work, we propose the use of complex, bioderived nanostructures as efficient surface-enhanced Raman scattering (SERS) substrates for chemical analysis cellular membranes. These structures were directly obtained from a suitable gold metalization Pseudonitzchia multistriata diatom silica shell (the so called frustule), whose grating-like geometry provides large light coupling with external radiation, whereas its extruded, subwavelength lateral edge an excellent interaction cells without...

A full label-free morphological and biochemical characterization is desirable to select spermatozoa during preparation for artificial insemination. In order study these fundamental parameters, we take advantage of two attractive techniques: digital holography (DH) Raman spectroscopy (RS). DH presents new opportunities studying aspect cells tissues non-invasively, quantitatively without the need staining or tagging, while RS a very specific technique allowing analysis cellular components with...

X- and Y-chromosome-bearing sperm cell sorting is of great interest, especially for animal production management systems genetic improvement programs. Here, we demonstrate an optical method based on Raman spectroscopy to separate cells, overcoming many the limitations associated with current sex-sorting protocols. A priori imaging bull spermatozoa was utilized select sampling points (head-neck region), which were then used discriminate cells a spectral classification model. Main variations...

Circulating tumor cells (CTCs) are that have penetrated the circulatory system preserving properties and heterogeneity. Detection characterization of CTCs has high potential clinical values many technologies been developed for CTC identification. These approaches remain challenged by extraordinary rarity difficulty efficiently distinguishing cancer from much larger number white blood in bloodstream. Consequently, there is still a need efficient rapid methods to capture broad spectrum...

Engineered gold nanoparticles (AuNPs) find application in several fields related to human activities (i.e., food and cosmetic industry or water purification) including medicine, where they are employed for diagnosis, drug delivery cancer therapy. As any material/reagent use, the safety of AuNPs needs accurate evaluation. prone contamination by bacterial endotoxin (lipopolysaccharide, LPS), a potent elicitor inflammatory responses mammals. It is therefore important, when assessing AuNP...

The diagnosis of male infertility is vastly complex. To date, morphology, motility, and concentration have been used as key parameters to establish the sperm normality achieve pregnancy both in natural assisted fecundation. However, spermatozoa from infertile men could present a variety alterations, such DNA fragmentation, alterations chromatin structure, aneuploidy, which demonstrated decrease reproductive capacity men. Therefore, ability see detailed relationships between morphology...

YBCO-like Nd123 and Gd1212 superconductors in the normal state, visible infrared regime, have been analyzed this work. Superconductors, particularly high-temperature (HTSCs), hold indeed a great interest highly innovative research field of metamaterials photonics with important applications. Indeed nanostructured devices suffer from increasing resistivity frequency metals, superconducting materials provide viable route to approach problem. Moreover, HTSCs are plasmonic at nonzero...

Currently, a combination of technologies is typically required to identify and classify leukemia cells. These methods often lack the specificity sensitivity necessary for early accurate diagnosis. Here, we demonstrate use Raman spectroscopy normal B cells, collected from healthy patients, three ALL cell lines (RS4;11, REH MN60 at different differentiation level, respectively). markers associated with DNA protein vibrational modes have been identified that exhibit excellent discriminating...

In this work we have used the Raman spectroscopy to identify and classify normal B cells, collected from different healthy patients, versus three acute lymphoblastic leukemia (B-ALL) cell lines (RS4;11, REH MN60), by analyzing their biochemical features. Moreover, studied effects of low-dose treatments methotrexate (MTX), a key drug generally in BALL maintenance therapy, all-trans-retinoic acid (ATRA), therapy leukemic disease [1]. We found regression B-ALL after MTX-treatments, while there...

This work relates to the problem of sex predetermination in animals based on separation Xand Y -bearing sperm cells before insemination. We developed a device for efficient, non-destructive and label-free sorting X-and their Raman spectroscopic characteristics. spectroscopy (RS) is non-invasive technique that allows biochemical analysis cellular components characterization molecular structure from stretching bending vibrational transitions. RS offers detailed information conformation,...

Semen analysis is widely used as diagnostic tool for assessing male fertility, controlling and managing the animal reproduction. The most important parameters measured in a semen are morphology biochemical alterations. For obtaining such information, non-invasive, label-free non-destructive techniques have to be used. Digital Holography (DH) combined with Raman Spectroscopy (RS) could represent perfect candidate rapid, high-sensitive morphological sperm cell analysis. In this study, DH-RS...

The ability to make an accurate diagnosis at the time of treatment is crucial for many diseases. However, current standard diagnostic procedures can only be performed in specialised healthcare facilities. To bring methods from a laboratory point treatment, alternative have been proposed. One them surfaceenhanced Raman scattering (SERS), which offers advantageous features such as high sensitivity biotarget detection and higher accuracy. Here, we developed advanced SERS platform...

Here we have developed an advanced surface-enhanced Raman scattering (SERS) platform that enables the ultrasensitive, rapid and highly specific identification of tumour biomarkers in liquid biopsies. Our focus is on detection Thyroglobulin (Tg), most important biomarker for diagnosis prognosis thyroid cancer. Specifically, SERS-active substrates fabricated by nanosphere lithography chip or tips optical fiber (OF) were functionalised with Tg Capture antibodies. Gold nanoparticles...