Hepatocellular carcinoma (HCC) is characterized by a propensity for multifocality, growth local spread, and dysregulation of multiple signaling pathways. These features may be determined the tumoral microenvironment. The potential tumor cells to modulate HCC behavior secreted proteins has been extensively studied. In contrast, genetic modulation poorly understood. We investigated role involvement tumor-derived nanovesicles capable altering gene expression their ability cell biological...

Hepatocellular cancers (HCC) are highly resistant to chemotherapy. TGFβ has been associated with chemoresistance in some human but the mechanisms involved unknown. We explored how might contribute altered responses therapy by assessing involvement and mechanistic contribution of extracellular vesicle long non-coding RNA (lncRNA) mediating TGFβ-dependent chemoresistance. reduced sensitivity HCC cells sorafenib or doxorubicin release both vesicles selected lncRNA within these vesicles. Amongst...

Although the expression of long noncoding RNA (lncRNA) is altered in hepatocellular cancer (HCC), their biological effects are poorly defined. We have identified lncRNA with highly conserved sequences, ultraconserved (ucRNA) that transcribed and HCC. Extracellular vesicles, such as exosomes microvesicles, released from tumor cells can transfer biologically active proteins across cells. sought to identify role vesicle-mediated ucRNA a mechanism by which these novel could influence...

Patients with advanced hepatocellular carcinoma (HCC) face a dismal prognosis because of lack any effective therapies. To address this situation, we conducted preclinical investigation the therapeutic efficacy oligonucleotides directed against oncogenic microRNA miR-221, which has been implicated in HCC. Of 9 chemistries evaluated, determined that 2'-O-methyl phosphorothioate-modified anti-miR-221 oligonucleotide was most at reducing proliferation vitro. A cholesterol-modified isoform...

Although expression of non–protein-coding RNA (ncRNA) can be altered in human cancers, their functional relevance is unknown. Ultraconserved regions are noncoding genomic segments that 100% conserved across humans, mice, and rats. Conservation gene sequences species may indicate an essential role, therefore we evaluated the ultraconserved RNAs (ucRNA) hepatocellular cancer (HCC). The global ucRNAs was analyzed with a custom microarray. Expression verified cell lines by real-time PCR or...

Pancreatic stellate cells (PSCs) interact with pancreatic cancer in the tumor microenvironment. Cell constituents including microRNAs may be exported from within membranous nanovesicles termed exosomes. Exosomes might play a pivotal role intercellular communication. This study aimed to clarify microRNA signature of PSC-derived exosomes and their effects on cells.Exosomes were prepared conditioned medium immortalized human PSCs. MicroRNAs source PSCs, expression profiles compared by...

The role of long noncoding RNAs (lncRNAs) in the epithelial-mesenchymal transition (EMT) pancreatic ductal adenocarcinoma (PDAC) is unclear. Some lncRNAs can be transferred by extracellular vesicles (EVs) and have potential as biomarkers. Here, we identify an lncRNA that could serve a biomarker for PDAC show functional roles lncRNA. Expression profiling revealed highly upregulated liver cancer (HULC) was expressed, induced, transforming growth factor-β cells their EVs. Knockdown HULC...

Abstract Myeloid-derived suppressor cells (MDSCs) could have important roles in immune regulation, and MDSCs can be induced patients with various malignant tumors. The immune-suppressive functions of hepatocellular carcinoma (HCC) not been clarified. Therefore, we tried to analyze the biological significance HCC patients. We quantified PD-L1 + conditions by using multi-color flow cytometry analysis. PBMCs from contained significantly higher percentages comparison those healthy subjects ( p...

MicroRNAs are small endogenous RNA molecules with specific expression patterns that can serve as biomarkers for numerous diseases. However, little is known about the profile of serum miRNAs in PBC.First, we employed Illumina deep sequencing initial screening to indicate read numbers miRNA 10 PBC, 5 CH-C, CH-B patients and healthy controls. Comparing differentially expressed 4 groups, analysis variance was performed on number sequence reads evaluate statistical significance. Hierarchical...

Abstract Some patients with ulcerative colitis (UC) suffer from complicated UC-associated upper gastrointestinal lesions. However, the trigger of these lesions has not been clarified. Herein, we present a 28-year-old man relapsed pan-colonic UC accompanied by gastroduodenal immediately after contracting coronavirus disease 2019 (COVID-19). In this patient, approximately 7 days COVID-19 onset, despite being in remission for 3 years. The patient also developed symptoms such as epigastric pain...

Accumulating evidence suggests that cancer stem cells (CSC) play an important role in tumorigenicity. Epithelial cell adhesion molecule (EpCAM) is one of the markers identifies tumor with high The expression EpCAM liver progenitor prompted us to investigate whether CSC could be identified hepatocellular carcinoma (HCC) lines. sorted + subpopulation from HCC lines showed a greater colony formation rate than − same lines, although proliferation was comparable between two subpopulations. vivo...

Accumulating evidence suggests the therapeutic potential of immunosuppressive agent FTY720 (fingolimod) in hepatocellular carcinoma (HCC). Based on our previous finding that mediates apoptosis HCC cells by activating reactive oxygen species (ROS)–protein kinase Cδ (PKCδ) signaling independent effects sphingosine-1-phosphate (S1P) receptors, we embarked pharmacological exploitation to develop a nonimmunosuppressive analogue with antitumor activity. This effort led development OSU-2S, which...

Background & AimsAlthough hepatocellular cancers (HCC) frequently arise in the setting of fibrosis and a hepatic regenerative response requiring new cell growth, therapeutic strategies for these have not targeted protein synthesis. Silvestrol, rocaglate isolated from Aglaia foveolata, can inhibit synthesis by modulating initiation translation through eukaryotic factor 4A. In this study, we evaluated efficacy silvestrol HCC. MethodsThe was examined using human HCC cells vitro an orthotopic...

Abstract Micro RNA s (mi s) are small, regulatory non‐coding that have potent effects on gene expression. Several mi deregulated in cellular processes involved human liver diseases and regulation of processes. Recent studies identified the involvement R ‐29 hepatic fibrosis carcinogenesis. Although several targets been identified, there is limited information regarding cell‐type specific roles liver, we sought to evaluate role this pathobiology. We report generation a tissue–specific...

Summary Micro RNA s were first discovered as small endogenous molecules and some viruses have been reported to interact with host mi s. By investigating expression in serum derived from HBV ‐infected patients, we clarified the relationship between chronic infection. Additionally, demonstrate use of both novel biomarkers new therapies against . We included sera 20 patients infection, HCV infection 10 healthy controls this study. The libraries sequenced using a 32‐mer single end sequence....

Hepatitis B virus (HBV) infection is a worldwide health problem because of its potential to cause liver cirrhosis and hepatocellular carcinoma. Silibinin constituent an extract milk thistle, which empirically used as herbal medicine for the protection liver, but detailed effects on HBV are unknown. Because previous study reported that silibinin hinders clathlin-mediated endocytosis (CME), we aimed test whether inhibits entry into hepatocytes. Using HepG2-NTCP-C4 cells, overexpress sodium...

Increasing evidence supports the important role of cancer stem cells (CSCs). Many reports suggest that epithelial cell adhesion molecule (EpCAM) is a useful marker for in hepatocellular carcinoma (HCC). To elucidate mechanisms cells, development specific molecular targeted drugs has become very important. In present study, we examined EpCAM expression pattern and its characteristic resected HCC. We studied drug resistance cells. was detected significantly more frequently with hepatitis B...

Aim Epithelial–mesenchymal transition ( EMT ) is a crucial process during cancer invasion and metastasis, which accompanied by the suppressed expression of E ‐cadherin initiated stimuli such as transforming growth factor TGF )‐β. Recent studies have shown that epigenetic regulation could be an alternate mechanism induction in hepatocellular carcinoma HCC ). mi RNA ‐29a miR ‐29a) involved genes targeting DNA methyltransferases DNMT ), methylate CpG islands to suppress transcription genes. We...

Small-for-size grafts are an issue in liver transplantation. Portal venous pressure (PVP) was monitored and intentionally controlled during living-donor transplantation (LDLT) 155 adult recipients. The indocyanine green elimination rate (kICG) simultaneously measured 16 recipients divided by the graft weight (g) to reflect portal flow (PVF). target PVP <20 mmHg. Patients were final (mmHg): Group A, < 12; B, 12 ≤ 15; C, 15 20; D, ≥ 20. With intentional control, we performed splenectomy...

Viruses are considered to use vesicular trafficking in infected cells, but the details of assembly/release pathways hepatitis B virus (HBV) still unknown. To identify key regulators HBV production, we performed short interfering RNA (siRNA) screening for Rab proteins, which act as molecular switches using HepG2.2.15 cells. Among 62 suppression Rab5B most significantly increased DNA culture supernatant. Surprisingly, 5 days after transfection siRNA, supernatant was more than 30-fold,...

Background The need for new therapies cholangiocarcinoma is highlighted by their poor prognosis and refractoriness to chemotherapy. Increased production of Interleukin-6 promotes growth contributes chemoresistance activating cell survival mechanisms. We sought identify biologically active compounds capable ameliorating the phenotypic effects IL-6 expression explore potential therapeutic use cholangiocarcinoma. Methodology A genomic signature associated with in Mz-ChA-1 human malignant...