A5058389506- Pharmacogenetics and Drug Metabolism
- Drug Transport and Resistance Mechanisms
- Carcinogens and Genotoxicity Assessment
- DNA Repair Mechanisms
- DNA and Nucleic Acid Chemistry
- Eicosanoids and Hypertension Pharmacology
- Metabolomics and Mass Spectrometry Studies
- Computational Drug Discovery Methods
- Analytical Chemistry and Chromatography
- Chemical Reactions and Isotopes
- Glutathione Transferases and Polymorphisms
- Cancer Treatment and Pharmacology
- Hormonal Regulation and Hypertension
- Drug-Induced Hepatotoxicity and Protection
- Metabolism and Genetic Disorders
- Genomics, phytochemicals, and oxidative stress
- Metal-Catalyzed Oxygenation Mechanisms
- Estrogen and related hormone effects
- Synthesis and Biological Evaluation
- HIV/AIDS drug development and treatment
- Hormonal and reproductive studies
- Cancer therapeutics and mechanisms
- Mycotoxins in Agriculture and Food
- Steroid Chemistry and Biochemistry
- Alcohol Consumption and Health Effects
Vanderbilt University
2016-2025
Vanderbilt-Ingram Cancer Center
2021
Konkuk University
2016-2021
Cairo University
2019
Bethany College - West Virginia
2018
Telio (Norway)
2018
Elisa (Finland)
2018
The University of Texas at San Antonio
2018
Osaka Prefectural Institute of Public Health
1986-2016
Showa Pharmaceutical University
2007-2016
Interindividual variations in the level and activity of cytochrome P-450 enzymes were investigated liver microsomes 30 Japanese Caucasian patients. The used this study included 1A2, 2A6, 2B6, 2C, 2D6, 2E1 3A, monooxygenase activities determined 13 typical substrates 9 procarcinogens. Although total content was higher (mean, 0.43 nmol/mg protein) than populations 0.26 protein), relative levels (percent P-450) individual forms immunochemically not very different except that 2A6 2B6 Caucasians....
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPurification and characterization of liver microsomal cytochromes P-450: electrophoretic, spectral, catalytic, immunochemical properties inducibility eight isozymes isolated from rats treated with phenobarbital or .beta.-naphthoflavoneF. Peter Guengerich, Ghazi A. Dannan, S. Tracy Wright, Martha V. Martin, Laurence KaminskyCite this: Biochemistry 1982, 21, 23, 6019–6030Publication Date (Print):November 9, 1982Publication History Published online1...
Genetic variants that are associated with common human diseases do not lead directly to disease, but instead act on intermediate, molecular phenotypes in turn induce changes higher-order disease traits. Therefore, identifying the vary response DNA and also associate traits has potential provide functional information required only identify validate susceptibility genes affected by DNA, understand networks which such operate how these Toward end, we profiled more than 39,000 transcripts...
A nomenclature for the P450 gene superfamily is proposed based on evolution. Recommendations include Roman numerals distinct families, capital letters subfamilies, and Arabic individual genes. An updating of this list, which presently includes 65 entries, will be required every 1–2 years. Assignment orthologous genes uncertain in some cases—between widely diverged species especially P450II family due to large number As more known, it might become necessary change assignments that are our...
Nifedipine oxidation has been shown to be catalyzed by cytochrome P-450 (P-450) enzymes.Reconstitution, immunoinhibition, and induction studies with rat liver indicated that the forms designated P -4 5 0 " ~. ~ P-~~O C N -E are major contributors microsomal nifedipine oxidation.The which oxidizes (P-45oNF) was purified electrophoretic homogeneity from several human samples.Antibodies raised P-45oNF were highly specific as judged immunoblotting analysis inhibited >90% of oxidase activity in...
Aromatic amines are well known as occupational carcinogens and found in cooked foods, tobacco smoke, synthetic fuels, agricultural chemicals. For the primary arylamines, metabolic N-oxidation by hepatic cytochromes P-450 is generally regarded an initial activation step leading to carcinogenesis. The of 4-aminobiphenyl, 2-naphthylamine, several heterocyclic has been shown recently be catalyzed rat cytochrome P-450ISF-G its human ortholog, P-450PA. We now report that microsomal caffeine...
complex has a characteristic absorption maximum (Soret band) near 450 nm due to axial ligation with cysteine thiolate of the protein (with or without substrate present). This residue is present in relatively well conserved region, -80% into from N terminus. Collectively there are thousands potential substrates for P-450s; each P-450s may have rather strict limitation catalytic specificity
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRegulation of rat hepatic cytochrome P-450: age-dependent expression, hormonal imprinting, and xenobiotic inducibility sex-specific isoenzymesDavid J. Waxman, Ghazi A. Dannan, F. Peter GuengerichCite this: Biochemistry 1985, 24, 16, 4409–4417Publication Date (Print):July 30, 1985Publication History Published online1 May 2002Published inissue 30 July...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemical mechanisms of catalysis by cytochromes P-450: a unified viewF. Peter Guengerich and Timothy L. MacdonaldCite this: Acc. Chem. Res. 1984, 17, 1, 9–16Publication Date (Print):January 1984Publication History Published online1 May 2002Published inissue 1 January 1984https://pubs.acs.org/doi/10.1021/ar00097a002https://doi.org/10.1021/ar00097a002research-articleACS PublicationsRequest reuse permissionsArticle Views887Altmetric-Citations426LEARN...
ADVERTISEMENT RETURN TO ISSUEPREVArticleEstimation of isozymes microsomal cytochrome P-450 in rats, rabbits, and humans using immunochemical staining coupled with sodium dodecyl sulfate-polyacrylamide gel electrophoresisF. Peter Guengerich, Philip Wang, Nancy K. DavidsonCite this: Biochemistry 1982, 21, 7, 1698–1706Publication Date (Print):March 30, 1982Publication History Published online1 May 2002Published inissue 30 March...
The human P450III family has been shown to be composed of at least four members, P450IIIA3 (HLp), P450IIIA4 (P450NF), P450IIIA5 (HLp3), and P450IIIA6 (HLp2). Due the lack probes that specifically recognize individual members this family, little is known about their relative expression. We prepared a form-specific antibody by immunoabsorption anti-P450IIIA5 IgG against Sepharose 4B upon which microsomes did not contain or purified had bound. Immunoblot analyses demonstrated was expressed...