A5035326361- Epigenetics and DNA Methylation
- Substance Abuse Treatment and Outcomes
- Alcohol Consumption and Health Effects
- Muscle metabolism and nutrition
- RNA modifications and cancer
- Behavioral Health and Interventions
- Genetics and Neurodevelopmental Disorders
- Gene expression and cancer classification
- Single-cell and spatial transcriptomics
- Genomics and Chromatin Dynamics
- Forensic Toxicology and Drug Analysis
- Tryptophan and brain disorders
- Immune responses and vaccinations
- Opioid Use Disorder Treatment
- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- Immune Cell Function and Interaction
- Smoking Behavior and Cessation
- Cannabis and Cannabinoid Research
- Frailty in Older Adults
- Genetic Syndromes and Imprinting
- Nutrition, Genetics, and Disease
- Genetics, Aging, and Longevity in Model Organisms
- Scoliosis diagnosis and treatment
- Telomeres, Telomerase, and Senescence
Laureate Institute for Brain Research
2023
Rush University Medical Center
2022
Virginia Tech Transportation Institute
2014-2018
Virginia Tech
2009-2018
The University of Sydney
2015
Walter Reed National Military Medical Center
2013
Merck & Co., Inc., Rahway, NJ, USA (United States)
2011
Illumina (United States)
2011
Rosetta Stone (United States)
2008
Genetic variants that are associated with common human diseases do not lead directly to disease, but instead act on intermediate, molecular phenotypes in turn induce changes higher-order disease traits. Therefore, identifying the vary response DNA and also associate traits has potential provide functional information required only identify validate susceptibility genes affected by DNA, understand networks which such operate how these Toward end, we profiled more than 39,000 transcripts...
Abstract Infinium Methylation BeadChips are widely used to profile DNA cytosine modifications in large cohort studies for reasons of cost-effectiveness, accurate quantification, and user-friendly data analysis characterizing these canonical epigenetic marks. In this work, we conducted a comprehensive evaluation the updated MethylationEPIC v2 BeadChip (EPICv2). Our revealed that EPICv2 offers significant improvements over its predecessors, including expanded enhancer coverage, applicability...
Abstract DNA methylation clocks can accurately estimate chronological age and, to some extent, also biological age, yet the process by which age-associated (DNAm) changes are acquired appears be quasi-stochastic, raising a fundamental question: how much of an epigenetic clock’s predictive accuracy could explained stochastic DNAm change? Here, using data from sorted immune cells, we build realistic simulation models, subsequently demonstrating in over 22,770 and whole-blood samples 25...
Tumorigenesis involves multistep genetic alterations. To elucidate the microRNA (miRNA)–gene interaction network in carcinogenesis, we examined their genome‐wide expression profiles 96 pairs of tumor/non‐tumor tissues from hepatocellular carcinoma (HCC). Comprehensive analysis coordinate miRNAs and mRNAs reveals that miR‐122 is under‐expressed HCC increased seed‐matched genes leads to a loss mitochondrial metabolic function. Furthermore, secondary targets, which decrease expression, are good...
We sought to determine the skeletal muscle genome-wide DNA methylation and mRNA responses one bout of lower load (LL) versus higher (HL) resistance exercise. Trained college-aged males (
Aging | doi:10.18632/aging.205581. Edwin Lee, Natàlia Carreras-Gallo, Leilani Lopez, Logan Turner, Aaron Lin, Tavis L. Mendez, Hannah Went, Alan Tomusiak, Eric Verdin, Michael Corley, Lishomwa Ndhlovu, Ryan Smith, Varun B. Dwaraka
The host epigenetic landscape rapidly changes during SARS-CoV-2 infection, and evidence suggest that severe COVID-19 is associated with durable scars to the epigenome. Specifically, aberrant DNA methylation in immune cells alterations clocks blood relate COVID-19. However, a longitudinal assessment of states from healthy individuals prior following test-confirmed non-hospitalized has not been performed. Moreover, impact mRNA vaccines upon epigenome remains understudied. Here, we first...
ABSTRACT Biological aging is a multifactorial process involving complex interactions of cellular and biochemical processes that reflected in omic profiles. Using common clinical laboratory measures ~30,000 individuals from the MGB-Biobank, we developed robust, predictive biological phenotype, EMRAge , balances biomarkers with overall mortality risk can be broadly recapitulated across EMRs. We then applied elastic-net regression to model DNA-methylation (DNAm) multiple omics, generating...
Abstract Background Geroscience focuses on interventions to mitigate molecular changes associated with aging. Lifestyle modifications, medications, and social factors influence the aging process, yet complex mechanisms require an in-depth exploration of epigenetic landscape. The specific clock predictor effects a vegan diet, compared omnivorous remain underexplored despite potential impacts aging-related outcomes. Methods This study examined impact entirely plant-based or healthy diet over 8...
Abstract Reactivation of retroelements in the human genome has been linked to aging. However, whether epigenetic state specific can predict chronological age remains unknown. We provide evidence that locus‐specific retroelement DNA methylation be used create retroelement‐based clocks accurately measure immune system, across tissues, and pan‐mammalian species. also developed a highly accurate clock compatible with EPICv.2.0 data was constructed from CpGs did not overlap existing first‐...
OBJECTIVE Surgery for spinal deformity has the potential to improve pain, disability, function, self-image, and mental health. These surgical procedures carry significant risk require careful selection, optimization, assessment. Epigenetic clocks are age estimation tools derived by measuring methylation patterns of specific DNA regions. The study biological in adult population shed insight onto molecular basis frailty current assessment tools. METHODS Adult patients who underwent surgery...
Although transcriptome profiling has been used in several resistance training studies, the associated analytical approaches seldom provide in-depth information on individual genes linked to skeletal muscle hypertrophy. Therefore, a secondary analysis was performed herein transcriptomic dataset we previously published involving trained college-aged men (n = 11) performing two exercise bouts randomized and crossover fashion. The lower-load bout (30 Fail) consisted of 8 sets lower body...
X-chromosome inactivation (XCI) is a fundamental mechanism in placental mammals that compensates for gene dosage differences between sexes. Using methylation levels of genes under XCI, we establish defective XCI as new source interindividual variation among cancer types females, characterized by significant and consistent lowering XIST expression enrichment differentially expressed XCI. We show an additive factor to the risk escape deregulation women. Defective more than 10% has attributable...
Aging interventions have progressed in recent years due to the growing curiosity about how lifestyle impacts longevity. This study assessed effects of SRW Laboratories' Cel System nutraceutical range on epigenetic methylation patterns, inflammation, physical performance, body composition, and biomarkers aging. A 1-year was conducted with 51 individuals, collecting data at baseline, 3 months, 6 12 months. Participants were encouraged walk 10 minutes practice 5 mindfulness daily. Significant...
Introduction: Interleukin-11 (IL-11) is a cytokine involved in inflammatory processes and previous study showed that blocking or knocking down IL11 mice prolongs healthy lifespan. This investigates DNA methylation (DNAm) changes the IL-11 receptor subunit alpha (IL-11RA) gene across ages to reveal how aging might influence production sensitivity. Methods: A genome-wide DNAm database focusing on Cytosine-phosphate-Guanine (CpG) sites within IL11RA was analyzed. Hierarchical regression...
Tobacco, alcohol, and marijuana consumption is an important public health problem because of their high use worldwide association with the risk mortality many conditions, such as hypertension, which commonest factor for death throughout world. A likely pathway action substance leading to persistent hypertension DNA methylation. Here, we evaluated effects tobacco, on methylation in same cohort (N = 3,424). Three epigenome-wide studies (EWAS) were assessed whole blood using...
Abstract Background Obesity and neurodevelopmental delay are complex traits that often co-occur differ between boys girls. Prenatal exposures believed to influence children’s obesity, but it is unknown whether of pregnant mothers can confer a different risk obesity sexes, they affect neurodevelopment. Methods We analyzed data from 1044 children the HELIX project, comprising 93 during pregnancy, clinical, neuropsychological, methylation childhood (5–11 years). Using exposome-wide interaction...
mRNA profiling has become an important tool for developing and validating prognostic assays predictive of disease treatment response outcome. Archives annotated formalin-fixed paraffin-embedded tissues (FFPET) are available as a potential source retrospective studies. Methods needed to profile these FFPET samples that linked clinical outcomes generate hypotheses could lead classifiers applications.We developed two-color microarray-based platform by optimizing target amplification,...
Abstract Background Differentially methylated imprint control regions (ICRs) regulate the monoallelic expression of imprinted genes. Their epigenetic dysregulation by environmental exposures throughout life results in formation common chronic diseases. Unfortunately, existing Infinium methylation arrays lack ability to profile these adequately. Whole genome bisulfite sequencing (WGBS) is unique method able ICRs. However, it very expensive and requires not only a high coverage, but also...
Aging interventions have progressed in recent years due to the growing curiosity about how lifestyle impacts longevity. This study assessed effects of SRW Laboratories Cel1, Cel2, and Cel3 (Cel System) nutraceutical range on epigenetic methylation patterns, inflammation, physical performance, body composition, biomarkers aging. A 1-year was conducted with 51 individuals, collecting data at baseline, 3 months, 6 12 months. Participants were encouraged walk 10 minutes practice 5 mindfulness...
The lack of accurate, cost-effective, and clinically relevant biomarkers remains a major barrier to incorporating omic data into clinical practice. Previous studies have shown that DNA methylation algorithms utility as surrogate measures for selected proteins metabolites. We expand upon this work by creating DNAm surrogates, termed epigenetic biomarker proxies (EBPs), across laboratories, the metabolome, proteome. After screening >2,500 biomarkers, we trained tested 1,694 EBP models assessed...
Objectives: Operation Building Resilience and Valuing Empowered Families (OBF) is a preventive, preclinical program that was developed by the Walter Reed National Military Medical Center's Child Adolescent Psychiatry Service to form working partnership with families of wounded warrior parents from war in Iraq Afghanistan. The OBF staff helps identify families' needs collaborates many organizations at Center provide assistance. This article describes OBF, offers case description, reviews...